Desmocollin 2 (DSC2) (NM_004949) Human Untagged Clone

CAT#: SC112671

DSC2 (untagged)-Human desmocollin 2 (DSC2), transcript variant Dsc2b

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Desmocollin 2
• Synonyms: ARVD11; CDHF2; DG2; DGII/III; DSC3
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004949, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAGGCAGCCCGCCCCTCCGGCTCCTGGAACGGAGCCCTCTGCCGGCTGCTCCTGCTGACCCTCGCGA
  TCTTAATATTTGCCAGTGATGCCTGCAAAAATGTGACATTACATGTTCCCTCCAAACTAGATGCCGAGAA
  ACTTGTTGGTAGAGTTAACCTGAAAGAGTGCTTTACAGCTGCAAATCTAATTCATTCAAGTGATCCTGAC
  TTCCAAATTTTGGAGGATGGTTCAGTCTATACAACAAATACTATTCTATTGTCCTCGGAGAAGAGAAGTT
  TTACCATATTACTTTCCAACACTGAGAACCAAGAAAAGAAGAAAATATTTGTCTTTTTGGAGCATCAAAC
  AAAGGTCCTAAAGAAAAGACATACTAAAGAAAAAGTTCTAAGGCGCGCCAAGAGAAGATGGGCTCCAATT
  CCTTGTTCGATGCTAGAAAACTCCTTGGGTCCTTTTCCACTTTTCCTTCAACAGGTTCAATCTGACACGG
  CCCAAAACTATACCATATACTATTCCATAAGAGGTCCTGGAGTTGACCAAGAACCTCGGAATTTATTTTA
  TGTGGAGAGAGACACTGGAAACTTGTATTGTACTCGTCCTGTAGATCGTGAGCAGTATGAATCTTTTGAG
  ATAATTGCCTTTGCAACAACTCCAGATGGGTATACTCCAGAACTTCCACTGCCCCTAATAATCAAAATAG
  AGGATGAAAATGATAACTACCCAATTTTTACAGAAGAAACTTATACTTTTACAATTTTTGAAAATTGCAG
  AGTGGGCACTACTGTGGGACAAGTGTGTGCTACTGACAAAGATGAGCCTGACACGATGCACACACGCCTG
  AAGTACTCCATCATTGGGCAGGTGCCACCATCACCCACCCTATTTTCTATGCATCCAACTACAGGCGTGA
  TCACCACAACATCATCTCAGCTAGACAGAGAGTTAATTGACAAGTACCAGTTGAAAATAAAAGTACAAGA
  CATGGATGGTCAGTATTTTGGTCTACAGACAACTTCAACTTGTATCATTAACATTGATGATGTAAATGAC
  CACTTGCCAACATTTACTCGTACTTCTTATGTGACATCAGTGGAAGAAAATACAGTTGATGTGGAAATCT
  TACGAGTTACTGTTGAGGATAAGGACTTAGTGAATACTGCTAACTGGAGAGCTAATTATACCATTTTAAA
  GGGCAATGAAAATGGCAATTTTAAAATTGTAACAGATGCCAAAACCAATGAAGGAGTTCTTTGTGTAGTT
  AAGCCTTTGAATTATGAAGAAAAGCAACAGATGATCTTGCAAATTGGTGTAGTTAATGAAGCTCCATTTT
  CCAGAGAGGCTAGTCCAAGATCAGCCATGAGCACAGCAACAGTTACTGTTAATGTAGAAGATCAGGATGA
  GGGCCCTGAGTGTAACCCTCCAATACAGACTGTTCGCATGAAAGAAAATGCAGAAGTGGGAACAACAAGC
  AATGGATATAAAGCATATGACCCAGAAACAAGAAGTAGCAGTGGCATAAGGTATAAGAAATTAACTGATC
  CAACAGGGTGGGTCACCATTGATGAAAATACAGGATCAATCAAAGTTTTCAGAAGCCTGGATAGAGAGGC
  AGAGACCATCAAAAATGGCATATATAATATTACAGTCCTTGCATCAGACCAAGGAGGGAGAACATGTACG
  GGGACACTGGGCATTATACTTCAAGACGTGAATGATAACAGCCCATTCATACCTAAAAAGACAGTGATCA
  TCTGCAAACCCACCATGTCATCTGCGGAGATTGTTGCGGTTGATCCTGATGAGCCTATCCATGGCCCACC
  CTTTGACTTTAGTCTGGAGAGTTCTACTTCAGAAGTACAGAGAATGTGGAGACTGAAAGCAATTAATGAT
  ACAGCAGCACGTCTTTCCTATCAGAATGATCCTCCATTTGGCTCATATGTAGTACCTATAACAGTGAGAG
  ATAGACTTGGCATGTCTAGTGTCACTTCATTGGATGTTACACTGTGTGACTGCATTACCGAAAATGACTG
  CACACATCGTGTAGATCCAAGGATTGGCGGTGGAGGAGTACAACTTGGAAAGTGGGCCATCCTTGCAATA
  TTGTTGGGCATAGCATTGCTCTTTTGCATCCTGTTTACGCTGGTCTGTGGGGCTTCTGGGACGTCTAAAC
  AACCAAAAGTAATTCCTGATGATTTAGCCCAGCAGAACCTAATTGTATCAAACACAGAAGCTCCTGGAGA
  TGACAAAGTGTATTCTGCGAATGGCTTCACAACCCAAACTGTGGGCGCTTCTGCTCAGGGAGTTTGTGGC
  ACCGTGGGATCAGGAATCAAAAACGGAGGTCAGGAGACCATCGAAATGGTGAAAGGAGGACACCAGACCT
  CGGAATCCTGCCGGGGGGCTGGCCACCATCACACCCTGGACTCCTGCAGGGGAGGACACACGGAGGTGGA
  CAACTGCAGATACACTTACTCGGAGTGGCACAGTTTTACTCAGCCCCGTCTTGGTGAAGAATCCATTAGA
  GGACACACTCTGATTAAAAATTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004949 unedited
  GGGTCANAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGATGGGCCT
  TATAGGTGGCGTCCGCTGGTGAGAAACACCTTGCGCAGTAAAAGGTGGCGGCGAGAGGGA
  GTTCCCACCCGTGGCTTTCTTAGAGAAATGAAGTCTTAAGTCTTAAATAGACAACAAGGA
  GGGGCCGATCGGTGTCTTTTGGACGCGTCTGGAGCCCCTCCCTCCGCCAAAGGAAAAGCC
  CCTTGGATGAGAGGCAGGCGCTTCAGAGAAGCTAAGAAAAGCACCTCTCCGCGCGCCCCA
  CCTCCTCCGCCTCGCGCTCCTCCTGAGCAGCGGGCCCAGACTGCGCTCCGGCCGCGGCCC
  TCGCCCCGCGGAGCCCTCCTACCCCGGCCCGACGCTCGGCCCGCGACCTGCCCCGAGCCC
  TCTCCATGGAGGCAGCCCGCCCCTCCGGCTCCTGGAACGGAGCCCTCTGCCGGCTGCTCC
  TGCTGACCCTCGCGATCTTAATATTTGCCAGTGATGCCTGCAAAAATGTGACATTACATG
  TTCCCTCCAAACTAGATGCCGAGAAACTTGTTGGTAGAGTTAACCTGAAAGAGTGCTTTA
  CAGCTGCAAATCTAATTCATTCAAGTGATCCTGACTTCCAAATTTTGGAGGATGGTTCAG
  TCTATACAACAAATACTATTCTATTGTCCTNCGAGAAGAGAAGTTNTACCATATTACTTT
  CNCACACTGAGAAACCAAGANAGAAAGAAATATTTNGTCTTTTTGGAGCATCANACANAG
  GTCCCCTAAAGAAAGACATACTATAGAAAAAGTTCTAAGGCGCGCCCAGAGAAGATGGGC
  TNCCATTCCTTGTTCGATGCTAGAAAACTNCTTGGGTCCTTTTTNCACTTTTCTTTNCAC
  AGGNTCATCTGACACGGCCCANAACTATACATATACTATT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004949 unedited
  NCCCCTCNTATGNNACCGCGGCCGCAATTTANNATCGAGTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTATGACTAGCCATTTTATCTAAGTTATAAAATAGTTACCCCAGGGAG
  CCTTCAAGATCCCTATATTTTAAAGCTTATTTCAAAAACCATCACTACTTCACATTTTTA
  AACTGCATCCTTGCATTACAGAACTGTAAATGGGTTGTCATACAGTAAATATCCACATCC
  CAATTCTAATTACACCATTCTTGTTGTTAACACAGAGCACTGCTTCTAATAAAAGCATGG
  CATAGTTCTACAGTTTCAATGGCAAAATTAAAGGTTTTTTTTCTTGAAGAGATAATGAAG
  CACTCACCAAGATATTTGAAACAACAAAGTTAACATTACAATGATTTCAAGAAAAACTAA
  GAATATTTCCCCTTACCTAAAACAAAAAAAAGGAATAATTCCCTTTATGAGAGAAGAAAA
  GAGAGACAGATTTTTAAAATATTTGGATTCTTTAGCCCATATGGAAATTAAAAAAAAAAA
  AAACCTGGGGAGAGAACCACAAGGAGACCGAGAGAAAGAGAGAGATGCCTCTTGACATTT
  TAAAGACCAAAAACTTGCAGCAGTTAAAATCCATTCCGGAAAGTTTTTTACAACCTTACA
  TTTCACCTGCCCAAAGCTCGTACCCGAGGAGACACCGATTCACTGCAGGATTTTATATAC
  ATTAAACCCTAAACTTAACCTCCCCCAAATGAAATTGTAATACACAATGCCTGGCTAAAA
  TTTTCCTTTGCCCGCGAAAAGGCCCCTCCCAAAACCACATTTCCCAGCATGCCGCCCCAC
  ACCTTCAAATGTCCCGCGAACACCGTTCTCCACCCCTCTCCACCCTTATTAACCGCGAAT
  ACGCTAACCAGGCCACTCCCATTCTCTTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004949
• Insert Size: 4640 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004949.2, NP_004940.1
• RefSeq Size: 5186 bp
• RefSeq ORF: 2544 bp
• Locus ID: 1824
• UniProt ID: Q02487
• Cytogenetics: 18q12.1
• Domains: CA
• Protein Families: Transmembrane
• Protein Pathways: Arrhythmogenic right ventricular cardiomyopathy (ARVC)
• Gene Summary: This gene encodes a member of the desmocollin protein subfamily. Desmocollins, along with desmogleins, are cadherin-like transmembrane glycoproteins that are major components of the desmosome. Desmosomes are cell-cell junctions that help resist shearing forces and are found in high concentrations in cells subject to mechanical stress. This gene is found in a cluster with other desmocollin family members on chromosome 18. Mutations in this gene are associated with arrhythmogenic right ventricular dysplasia-11, and reduced protein expression has been described in several types of cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2015]
  Transcript Variant: This variant (Dsc2b, also known as DGIII) contains an additional exon at the 3' end compared to transcript variant Dsc2a. This results in a frame-shift and a shorter isoform (Dsc2b) with a distinct C-terminus compared to isoform Dsc2a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.