P4HA1 (NM_000917) Human Untagged Clone

CAT#: SC110967

P4HA1 (untagged)-Human prolyl 4-hydroxylase, alpha polypeptide I (P4HA1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: P4HA1
• Synonyms: P4HA
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000917, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGATCTGGTATATATTAATTATAGGAATTCTGCTTCCCCAGTCTTTGGCTCATCCAGGCTTTTTTACTT
  CAATTGGTCAGATGACTGATTTGATCCATACTGAGAAAGATCTGGTGACTTCTCTGAAAGATTATATTAA
  GGCAGAAGAGGACAAGTTAGAACAAATAAAAAAATGGGCAGAGAAGTTAGATCGGCTAACTAGTACAGCG
  ACAAAAGATCCAGAAGGATTTGTTGGGCATCCAGTAAATGCATTCAAATTAATGAAACGTCTGAATACTG
  AGTGGAGTGAGTTGGAGAATCTGGTCCTTAAGGATATGTCAGATGGCTTTATCTCTAACCTAACCATTCA
  GAGACAGTACTTTCCTAATGATGAAGATCAGGTTGGGGCAGCCAAAGCTCTGTTACGTCTCCAGGATACC
  TACAATTTGGATACAGATACCATCTCAAAGGGTAATCTTCCAGGAGTGAAACACAAATCTTTTCTAACGG
  CTGAGGACTGCTTTGAGTTGGGCAAAGTGGCCTATACAGAAGCAGATTATTACCATACGGAACTGTGGAT
  GGAACAAGCCCTAAGGCAACTGGATGAAGGCGAGATTTCTACCATAGATAAAGTCTCTGTTCTAGATTAT
  TTGAGCTATGCGGTATATCAGCAGGGAGACCTGGATAAGGCACTTTTGCTCACAAAGAAGCTTCTTGAAC
  TAGATCCTGAACATCAGAGAGCTAATGGTAACTTAAAATATTTTGAGTATATAATGGCTAAAGAAAAAGA
  TGTCAATAAGTCTGCTTCAGATGACCAATCTGATCAGAAAACTACACCAAAGAAAAAAGGGGTTGCTGTG
  GATTACCTGCCAGAGAGACAGAAGTACGAAATGCTGTGCCGTGGGGAGGGTATCAAAATGACCCCTCGGA
  GACAGAAAAAACTCTTTTGCCGCTACCATGATGGAAACCGTAATCCTAAATTTATTCTGGCTCCAGCTAA
  ACAGGAGGATGAATGGGACAAGCCTCGTATTATTCGCTTCCATGATATTATTTCTGATGCAGAAATTGAA
  ATCGTCAAAGACCTAGCAAAACCAAGGCTGAGCCGAGCTACAGTACATGACCCTGAGACTGGAAAATTGA
  CCACAGCACAGTACAGAGTATCTAAGAGTGCCTGGCTCTCTGGCTATGAAAATCCTGTGGTGTCTCGAAT
  TAATATGAGAATACAAGATCTAACAGGACTAGATGTTTCCACAGCAGAGGAATTACAGGTAGCAAATTAT
  GGAGTTGGAGGACAGTATGAACCCCATTTTGACTTTGCACGGAAAGATGAGCCAGATGCTTTCAAAGAGC
  TGGGGACAGGAAATAGAATTGCTACATGGCTGTTTTATATGAGTGATGTGTCTGCAGGAGGAGCCACTGT
  TTTTCCTGAAGTTGGAGCTAGTGTTTGGCCCAAAAAAGGAACTGCTGTTTTCTGGTATAATCTGTTTGCC
  AGTGGAGAAGGAGATTATAGTACACGGCATGCAGCCTGTCCAGTGCTAGTTGGCAACAAATGGGTATCCA
  ATAAATGGCTCCATGAACGTGGACAAGAATTTCGAAGACCTTGTACGTTGTCAGAATTGGAATGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000917 unedited
  GTTCACCATTATGTAATACGACTTACTATAGGGNNCGGCCGCGCAATTCGGCACGAGGGC
  GGGCTGATGGTAGGAAGTAGCCGCTCCGAGTGGAGGCGACTGGGGGCTGAAGAGCGCGCC
  GCCCTCTCGTCCCACTTTCCAGGTGTGTGATCCTGTAAAATTAAATCTTCCAAGATGATC
  TGGTATATATTAATTATAGGAATTCTGCTTCCCCAGTCTTTGGCTCATCCAGGCTTTTTT
  ACTTCAATTGGTCAGATGACTGATTTGATCCATACTGAGAAAGATCTGGTGACTTCTCTT
  GAAAGATTATATTAAGGCAGAAGAGGACAAGTTAGAACAAATAAAAAAATGGGCAGAGAA
  GTTAGATCGGCTAACTAGTACAGCGACAAAAGATCCAGAAGGATTTGTTGGGCATCCAGT
  AAATGCATTCAAATTAATGAAACGTCTGAATACTGAGTGGAGTGAGTTGGAGAATCTGGT
  CCTTAAGGATATGTCAGATGGCTTTATCTCTAACCTAACCATTCAGAGACAGTACTTTCC
  TAATGATGAAGATCAGGTTGGGGCAGCCAAAGCTCTGTTACGTCTCCAGGATACCTACAA
  TTTGGATACAGATACCATCTCAAAGGGTAATCTTCCAGGAGTGAAACACANATCTTTTCT
  AACGGCTGAGGACTGCTTTGAGTTGGGCAAAGTGGCCTATACAGAAGCAGATTATTACCA
  TACGGAACTGTGGATGGAACAAGCCCTAANGCAACTGGATGAANGCGAGATTTCTACCAT
  AGATNAAGTCTCTGTTCTAGATTATTTGAGCTATGCCGTATATCAGCAGGGAGACCTGNN
  ATANGCACTTTTGCTCACAAANAAGCTTCTTGCACTAGACCCTGNACATCAGAGAGCTCA
  TGGTAACCTTAAATATTTTGAGTATATATGGCTAAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000917 unedited
  GACCGCGGACTCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTACTAAAAAGATTTAATACCATAAATCGGGCTTTGTTGCCA
  CAACCCTTTTCAAAATTTTAAAAAAACCTCTTTTTTGGCTTTTTAAAAAAAAAACTTTTA
  AAAAAAATAAATTCCCTCATCACTGAAAGGACTTGTACTTTTTTAAACTTCCAGTCTCCT
  AAGGCCCATTTTTTAATCAAAACTCCAATTTTACCCCCCTGCTTGTACAGGAATAAAAGG
  CAGGGTTTTTCCCCTAAAAAAAACAGCAAAATTCCTGAACAAAATTTTTTGTTTTTTTAA
  AAAAGGGATAAAAAACCCGGCTAAGGGGGGACCTTTTTATTTAAAAAGGCCCACTGTTGT
  TTTGCCGGACTTTCCGTTAAAAAAAAAAAAAAATTCGAATTATTTTATCTCAAGTCCCGT
  TTAATTTCCCCCCAGGCTAGCCTTAAAAAAAGAGAGCTGGGGTGTGGAAAATACAGTTCT
  CTTTTTTTAAAAAAGACTTGGACTGGAAAAAACCCCCTTAAAAAAATTTTGAAAGGGTAA
  CTCCATGGGACCCCTTTTGTGGCCCTGCTTTTTTACCACCTCTTCAAACCCAGAACCCAG
  ACTGTCACTCCTGGGGTACAAAAAACACCTAGGCACATTTGTAAGAGGTTTTGGTTTCTT
  TTCCACACGCCCTTTTTTGTTTTACCACCCGCCCCCTGTGAAGTGTGCGAATACCCACAC
  CTGGTTCTCATTTTTTTTTTTTCTCCACCACAAATTTTTTAAAATTAAAAACCTCCCCCT
  TTAAACCTTGGCCTCGTGTTTTGTGATACCCCCAATACTTTCAGGTGTTTTGGCTTCTGA
  GCACCCCGCGGTCCTTTTAAAACCCCCCCAGTTTCATATGTCCCCAAAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000917
• Insert Size: 3160 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000917.2, NP_000908.2
• RefSeq Size: 2752 bp
• RefSeq ORF: 1605 bp
• Locus ID: 5033
• UniProt ID: P13674
• Cytogenetics: 10q22.1
• Domains: 2OG-FeII_Oxy, P4Hc
• Protein Families: Druggable Genome, P450
• Protein Pathways: Arginine and proline metabolism, Metabolic pathways
• Gene Summary: This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) encodes isoform 1.