DNMT3L (NM_175867) Human Untagged Clone

CAT#: SC110409

DNMT3L (untagged)-Human DNA (cytosine-5-)-methyltransferase 3-like (DNMT3L), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DNMT3L
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC110409 sequence for NM_175867 edited (data generated by NextGen Sequencing)
  ATGGCGGCCATCCCAGCCCTGGACCCAGAGGCCGAGCCCAGCATGGACGTGATTTTGGTG
  GGATCCAGTGAGCTCTCAAGCTCCGTTTCACCCGGGACAGGCAGAGATCTTATTGCATAT
  GAAGTCAAGGCTAACCAGCGAAATATAGAAGACATCTGCATCTGCTGCGGAAGTCTCCAG
  GTTCACACACAGCACCCTCTGTTTGAGGGAGGGATCTGCGCCCCATGTAAGGACAAGTTC
  CTGGATGCCCTCTTCCTGTACGACGATGACGGGTACCAATCCTACTGCTCCATCTGCTGC
  TCCGGAGAGACGCTGCTCATCTGCGGAAACCCTGATTGCACCCGATGCTACTGCTTCGAG
  TGTGTGGATAGCCTGGTCGGCCCCGGGACCTCGGGGAAGGTGCACGCCATGAGCAACTGG
  GTGTGCTACCTGTGCCTGCCGTCCTCCCGAAGCGGGCTGCTGCAGCGTCGGAGGAAGTGG
  CGCAGCCAGCTCAAGGCCTTCTACGACCGAGAGTCGGAGAATCCCCTTGAGATGTTCGAA
  ACCGTGCCTGTGTGGAGGAGACAGCCAGTCCGGGTGCTGTCCCTTTTTGAAGACATCAAG
  AAAGAGCTGACGAGTTTGGGCTTTTTGGAAAGTGGTTCTGACCCGGGACAACTGAAGCAT
  GTGGTTGATGTCACAGACACAGTGAGGAAGGATGTGGAGGAGTGGGGACCCTTCGATCTT
  GTGTACGGCGCCACACCTCCCCTGGGCCACACCTGTGACCGTCCTCCCAGCTGGTACCTG
  TTCCAGTTCCACCGGCTCCTGCAGTACGCACGGCCCAAGCCAGGCAGCCCCGGGCCCTTC
  TTCTGGATGTTCGTGGACAATCTGGTGCTGAACAAGGAAGACCTGGACGTCGCATCTCGC
  TTCCTGGAGATGGAGCCAGTCACCATCCCAGATGTCCACGGCGGATCCTTGCAGAATGCT
  GTCCGCGTGTGGAGCAACATCCCAGCCATAAGGAGCAGGCACTGGGCTCTGGTTTCGGAA
  GAAGAATTGTCCCTGCTGGCCCAGAACAAGCAGAGCTCGAAGCTCGCGGCCAAGTGGCCC
  ACCAAGCTGGTGAAGAACTGCTTTCTCCCCCTAAGAGAATATTTCAAGTATTTTTCAACA
  GAACTCACTTCCTCTTTATAA
  
  Clone variation with respect to NM_175867.1
  832 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_175867 unedited
  GGAACTTGAGAATTGTAACACCACTTATATAGGCGGCCGCGAATTCGCACGAGGTGGGCC
  TGGNACCTTCCACTCATCTTTGCCTGCGCCGTCCCTCGTTCCTGGCCCAGTCCTCCCCGG
  GGACCCTGAGCCTGGAAGCCCCGGACCACTGGAACCTTGAACCCACCAGCTGGCTGTACC
  CGGAGCCGTGGCAGCAGCCCTCATCCCCATGGCGGCCATCCCAGCCCTGGACCCAGAGGC
  CGAGCCCAGCATGGACGTGATTTTGGTGGGATCCAGTGAGCTCTCAAGCTCCGTTTCACC
  CGGGACAGGCAGAGATCTTATTGCATATGAAGTCAAGGCTAACCAGCGAAATATAGAAGA
  CATCTGCATCTGCTGCGGAAGTCTCCAGGTTCACACACAGCACCCTCTGTTTGAGGGAGG
  GATCTGCGCCCCATGTAAGGACAAGTTCCTGGATGCCCTCTTCCTGTACGACGATGACGG
  GTACCAATCCTACTGCTCCATCTGCTGCTCCGGAGAGACGCTGCTCATCTGCGGAAACCC
  TGATTGCACCCGATGCTACTGCTTCGAGTGTGTGGATAGCCTGGTCGGCCCCGGGACCTC
  GGGGAAGGTGCACGCCATGAGCAACTGGGTGTGCTACCTGTGCCTGCCGTCCTCCCGAAG
  CGGGCTGCTGCAGCGTCGGAGGAAGTGGCGCAGCCAGCTCAAGGCCTTCTACGACCGAGA
  GTCGGAGAATCCCCTTGAGATGTTCGAAACCGTGCCTGTGTGGAGGAGACAGCCAGTCCG
  GGTGCTGTNCCTTTTTGAAGACATCAAGAAGACTGACGAGTTTGGGCTTTTTGGAAAAGT
  GGTTCTGACCCGGGACACTGAAGCATGTGGTTGATGTCACANACACGTGAGGAAAGATGT
  GNA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_175867 unedited
  CCTTGCGCCATTGGCGATGGTCAACTTGCCAGGGCCAGGAAAGCACTGGGGNAGGGTCAC
  AGGGCTGCCACCCGGGTTCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTATAGTC
  GAGTTTTTTTTTTTTTTTTTTTTTTTTCTTTGTTCAAAGAAAAGTCTTTTTCTTCACAGT
  ATAGTGACTCTTTTATAAAGAGGAAGTGAGTTCTGTTGAAAAATACTTGAAATTTTCTCT
  TAGGGGGAAAAAGCATTTCTTCACCAGCTTGGTGGGCCACTTGGCCGCGAGCTTCGAGCT
  CTGCTTGTTCTGGGCCAGCAGGGACATTTCTTCTTCCGAAACCAGAGCCCAGTGCCTGCT
  CCTTATGGCTGGGATGTTGCTCCACACGCGGACAGCATTCTGCAAGGCTCCGCCGTGGAC
  ATCTGGGATGGTGACTGGCTCCCTCTCCAGGAAGCGAGATGCGACGTCCAGGTCTTCCTT
  GTTCAGCACCAGATTGTCCACGAACATCCAAAAAAAGGGCCCGGGGCTGCCTGGCTTGGG
  CCGTGCGTACTGCAGGAGCCGGTGGAACTGGAACAGGTACCAGCTGGGAGGACGGTCACA
  GGTGTGGCCCAGGGAGAGGTGTGGCGCCGTACACAATATCGAAGGGTCCCCACTCCTCCA
  CATCCTTCCTCAGTGTGTCTGTGACATCAACCACATGCTTCAGTTGTCCCGGGTCAAATC
  CACTTTCCAAAAAGCCCAAACTCGTCAGCTCTTTCTTGATGTCTTCAAAAAGGGACAGCA
  CCCGGACTGGCTGTCTCCTCCCACAGGCACGGTTTCAAACTCTAAGGGGATTCTCGACTC
  TCGGTCGTAAAGGCCTTGAGCTGGCTGCNCCACTCCTCCGAGCTGCACAGCCCGTTCGGG
  AGGACG
  
• Restriction Sites: Please inquire
• ACCN: NM_175867
• Insert Size: 1300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175867.1, NP_787063.1
• RefSeq Size: 1702 bp
• RefSeq ORF: 1161 bp
• Locus ID: 29947
• UniProt ID: Q9UJW3
• Cytogenetics: 21q22.3
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Cysteine and methionine metabolism, Metabolic pathways
• Gene Summary: CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases, but is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, it does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012]
  Transcript Variant: This variant (2) uses an alternate in-frame acceptor splice site at the 3' terminal exon compared to variant 1, which results in an isoform (2) that is 1 aa shorter than isoform 1.