Beta TRCP (BTRC) (NM_033637) Human Untagged Clone

CAT#: SC110015

BTRC (untagged)-Human beta-transducin repeat containing (BTRC), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Beta TRCP
• Synonyms: BETA-TRCP; betaTrCP; bTrCP; bTrCP1; FBW1A; FBXW1; FBXW1A; FWD1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_033637 edited
  GAATTCGGCACGAGGCCCCGGCGGAGAGCGGACCCAGTGGCCTCGGCGATTATGGACCCG
  GCCGAGGCGGTGCTGCAAGAGAAGGCACTCAAGTTTATGTGCTCTATGCCCAGGTCTCTG
  TGGCTGGGCTGCTCCAGCCTGGCGGACAGCATGCCTTCGCTGCGATGCCTGTATAACCCA
  GGGACTGGCGCACTCACAGCTTTCCAGAATTCCTCAGAGAGAGAAGACTGTAATAATGGC
  GAACCCCCTAGGAAGATAATACCAGAGAAGAATTCACTTAGACAGACATACAACAGCTGT
  GCCAGACTCTGCTTAAACCAAGAAACAGTATGTTTAGCAAGCACTGCTATGAAGACTGAG
  AATTGTGTGGCCAAAACAAAACTTGCCAATGGCACTTCCAGTATGATTGTGCCCAAGCAA
  CGGAAACTCTCAGCAAGCTATGAAAAGGAAAAGGAACTGTGTGTCAAATACTTTGAGCAG
  TGGTCAGAGTCAGATCAAGTGGAATTTGTGGAACATCTTATATCCCAAATGTGTCATTAC
  CAACATGGGCACATAAACTCGTATCTTAAACCTATGTTGCAGAGAGATTTCATAACTGCT
  CTGCCAGCTCGGGGATTGGATCATATTGCTGAGAACATTCTGTCATACCTGGATGCCAAA
  TCACTATGTGCTGCTGAACTTGTGTGCAAGGAATGGTACCGAGTGACCTCTGATGGCATG
  CTGTGGAAGAAGCTTATCGAGAGAATGGTCAGGACAGATTCTCTGTGGAGAGGCCTGGCA
  GAACGAAGAGGATGGGGACAGTATTTATTCAAAAACAAACCTCCTGACGGGAATGCTCCT
  CCCAACTCTTTTTATAGAGCACTTTATCCTAAAATTATACAAGACATTGAGACAATAGAA
  TCTAATTGGAGATGTGGAAGACATAGTTTACAGAGAATTCACTGCCGAAGTGAAACAAGC
  AAAGGAGTTTACTGTTTACAGTATGATGATCAGAAAATAGTAAGCGGCCTTCGAGACAAC
  ACAATCAAGATCTGGGATAAAAACACATTGGAATGCAAGCGAATTCTCACAGGCCATACA
  GGTTCAGTCCTCTGTCTCCAGTATGATGAGAGAGTGATCATAACAGGATCATCGGATTCC
  ACGGTCAGAGTGTGGGATGTAAATACAGGTGAAATGCTAAACACGTTGATTCACCATTGT
  GAAGCAGTTCTGCACTTGCGTTTCAATAATGGCATGATGGTGACCTGCTCCAAAGATCGT
  TCCATTGCTGTATGGGATATGGCCTCCCCAACTGACATTACCCTCCGGAGGGTGCTGGTC
  GGACACCGAGCTGCTGTCAATGTTGTAGACTTTGATGACAAGTACATTGTTTCTGCATCT
  GGGGATAGAACTATAAAGGTATGGAACACAAGTACTTGTGAATTTGTAAGGACCTTAAAT
  GGACACAAACGAGGCATTGCCTGTTTGCAGTACAGGGACAGGCTGGTAGTGAGTGGCTCA
  TCTGACAACACTATCAGATTATGGGACATAGAATGTGGTGCATGTTTACGAGTGTTAGAA
  GGCCATGAGGAATTGGTGCGTTGTATTCGATTTGATAACAAGAGGATAGTCAGTGGGGCC
  TATGATGGAAAAATTAAAGTGTGGGATCTTGTGGCTGCTTTGGACCCCCGTGCTCCTGCA
  GGGACACTCTGTCTACGGACCCTTGTGGAGCATTCCGGAAGAGTTTTTCGACTACAGTTT
  GATGAATTCCAGATTGTCAGTAGTTCACATGATGACACAATCCTCATCTGGGACTTCCTA
  AATGATCCAGCTGCCCAAGCTGAACCCCCCCGTTCCCCTTCTCGAACATACACCTACATC
  TCCAGATAAATAACCATACACTGACCTCATACTTGCCCAGGACCCATTAAAGTTGCGGTA
  TTTAACGTATCTGCCAATACCAGGATGAGCAACAACAGTAACAATCAAACTACTGCCCAG
  TTTCCCTGGACTAGCCGAGGAGCAGGGCTTTGAGACTCCTGTTGGGACACAGTTGGTCTG
  CAGTCGGCCCAGGACGGTCTACTCAGCACAACTGACTGCTTCAGTGCTGCTATCAGAAGA
  TGTCTTCTATCTTTTGTGAATGATTGGAACTTTTAAACCTCCCCTCCTCTCCTCCTTTCA
  CCTCTGCACCTAGTTTTTTCCCATTGGTTCCAGACAAAGGTGACTTATAAATATATTTAG
  TGTTTTGCCAGAATCTCTCTTGCTTTGCCATTAAGCAGAAGAACTAGTTTCCCTGTATAG
  CCTGCTGGGAGAGACCCACTTCTAGGGTATGGGGGATGCAGCTTCAAGCCCAGTGCCCAG
  TGTCTCCCTGTTAACTGCAGGAATGCCAAGCACCTGGCCAGAGCAGCCCAGCCCCAATAT
  GCTTAGGAGGAGACAGAGTTCCCTCTGTATAGCCTCTGGGACAAGAAAAAGAAAACACAA
  GAATGTATACACTGGAAGATTTGGGCCTCCTGCCTGCCTTCTCTTTGTTTCTGTTCCTCT
  TCCCATCTACTCCCCTACGCCCCTTCAACCTTTTTTCTCTGTCTGCTTCACCTGAGAAGA
  AAGTGTACGAAGAGAGTGTCCTCCTCTCACATGAGCCAGATCAGCCAGAAAATGCAACAC
  TTGGAAGAGTTAAATGCTGTTCAGTGAAGATTTCAGCCCCAGGCCTTTGCTGCAAGTGAC
  CCTGTGGCAACAGTGGATTCTCAGACATGATACTCTCATCATATTTGCAACTCTTCTCTC
  TCTTTCTTCCCCACACCCAAGAGGAGGATTGGTGGTAGGGGGCAGGCAGAGGGGGTGGGG
  AGAAGTTTCCTGGGCTCCATCAATGGCTGCATCTTTTCTGGACTCAGCAGTCTCCTTGAT
  TCCATGTAGAGTGTGGAAAGGAGTTGCTGATTGCATTTCCTCTCATTAACAATTGGGTGT
  GTAATAAAAAGCATTGTACTTCATCTTAAAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_033637 unedited
  GCACATTTTGTAATACGACTCACTATAGGGCGGCCGCGAAATTCGCACGAGGCCCCGGCG
  GAGAGCGGACCCAGTGGCCTCGGCGATTATGGACCCGGCCGAGGCGGTGCTGCAAGAGAA
  GGCACTCAAGTTTATGTGCTCTATGCCCAGGTCTCTGTGGCTGGGCTGCTCCAGCCTGGC
  GGACAGCATGCCTTCGCTGCGATGCCTGTATAACCCAGGGACTGGCGCACTCACAGCTTT
  CCAGAATTCCTCAGAGAGAGAAGACTGTAATAATGGCGAACCCCCTAGGAAGATAATACC
  AGAGAAGAATTCACTTAGACAGACATACAACAGCTGTGCCAGACTCTGCTTAAACCAAGA
  AACAGTATGTTTAGCAAGCACTGCTATGAAGACTGAGAATTGTGTGGCCAAAACAAAACT
  TGCCAATGGCACTTCCAGTATGATTGTGCCCAAGCAACGGAAACTCTCAGCAAGCTATGA
  AAAGGAAAAGGAACTGTGTGTCAAATACTTTGAGCAGTGGTCAGAGTCAGATCAAGTGGA
  ATTTGTGGAACATCTTATATCCCAAATGTGTCATTACCAACATGGGCACATAAACTCGTA
  TCTTAAACCTATGTTGCAGAGAGATTTCATAACTGCTCTGCCAGCTCGGGGATTGGATCA
  TATTGCTGAGAACATTCTGTCATACCTGGATGCCAAATCACTATGTGCTGCTGAACTTGT
  GTGCAAGGAATGGTACCGAGTGACCTCTGATGGCATGCTGTGGAAGAAGCTTATCGAGAG
  AATGGTCAGGACAGATTCTCTGTGGAGAGGCCTGGCAGAACGAAAAGGATGGGGACAGTA
  TTTATTCAAAAACAACCTCCTGACGGGAATGCNTCTCCCACTCTTNTATAGAGCACTTAT
  CCTAAAATATACCAGACATTGAGACATAGAATCTATGGNAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_033637 unedited
  CAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTAAAATGAAGTACAATGCTTTTTATT
  ACACACCCAATTGTTAATGAGAGGAAATGCAATCAGCAACTCCTTTCCACACTCTACATG
  GAATCAAGGAGACTGCTGAGTCCAGAAAAGATGCAGCCATTGATGGAGCCCAGGAAACTT
  CTCCCCACCCCCTCTGCCTGCCCCCTACCACCAATCCTCCTCTTGGGTGTGGGGAAGAAA
  GAGAGAGAAGAGTTGCAAATATGATGAGAGTATCATGTCTGAGAATCCACTGTTGCCACA
  GGGTCACTTGCAGCAAAGGCCTGGGGCTGAAATCTTCACTGAACAGCATTTAACTCTTCC
  AAGTGTTGCATTTTCTGGCTGATCTGGCTCATGTGAGAGGAGGACACTCTCTTCGTACAC
  TTTCTTCTCAGGTGAAGCAGACAGAGAAAAAAGGTTGAAGGGGCGTAGGGGAGTATATGG
  GAAGAGGAACAGAAACAAAGAGAAGGCAGGCAGGAGGCCCAAATCTTCCAGTGTATACAT
  TCTTGAGTTTTCTTTTTCTTGTCCCAGAGGCTATACAGAGGGAACTCTGTCTCCTCCTAA
  GCATATTGAGGCTGGGCTGCTCTGGCCAGGTGCTTGGCATTCCTGCAGTTAACAGGGAGA
  CACTGGGCACTGGNGCTGAAGCTGCATCCCCCATACCCTAGAAGTGGGTCTCTCCCAGCA
  GCTATACAGGGAAACTAGTTCTTCTGCTTAATGGCAATGCAGAGAGATCCTGGCAAAACA
  CTAAATATATCTATAAGTCACCTTTNGTCTGGACAATGGGNAATAACTTAGTGCCAAGTG
  AAAGAGGAAAAGAGGGAGGTTTAAAAGTTCCATCATCCCAAAGAATAAGACTCTCCGAAA
  CAGCCTGAGCATCAGGTGGCTGATAAACGTCTGGGCGACTGANACACTGGTCCAAAAGGT
  CCTAAGCCGCTCTCGCTTTCAGGAACTGCACTATTGTGTCCTGTGTGTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_033637
• Insert Size: 3270 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033637.2, NP_378663.1
• RefSeq Size: 6146 bp
• RefSeq ORF: 1818 bp
• Locus ID: 8945
• UniProt ID: Q9Y297
• Cytogenetics: 10q24.32
• Domains: WD40, F-box
• Protein Families: Druggable Genome
• Protein Pathways: Hedgehog signaling pathway, Oocyte meiosis, Ubiquitin mediated proteolysis, Wnt signaling pathway
• Gene Summary: This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class; in addition to an F-box, this protein contains multiple WD-40 repeats. The encoded protein mediates degradation of CD4 via its interaction with HIV-1 Vpu. It has also been shown to ubiquitinate phosphorylated NFKBIA (nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha), targeting it for degradation and thus activating nuclear factor kappa-B. Alternatively spliced transcript variants have been described. A related pseudogene exists in chromosome 6. [provided by RefSeq, Mar 2012]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).