NPL (NM_030769) Human Untagged Clone

CAT#: SC109402

NPL (untagged)-Human N-acetylneuraminate pyruvate lyase (dihydrodipicolinate synthase) (NPL), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NPL
• Synonyms: C1orf13; C112; NAL; NPL1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109402 sequence for NM_030769 edited (data generated by NextGen Sequencing)
  ATGGCCTTCCCAAAGAAGAAACTTCAGGGTCTTGTGGCTGCAACCATCACGCCAATGACT
  GAGAATGGAGAAATCAACTTTTCAGTAATTGGTCAGTATGTGGATTATCTTGTGAAAGAA
  CAGGGAGTGAAGAACATTTTTGTGAATGGCACAACAGGAGAAGGCCTGTCCCTGAGCGTC
  TCAGAGCGTCGCCAGGTTGCAGAGGAGTGGGTGACAAAAGGGAAGGACAAGCTGGATCAG
  GTGATAATTCACGTAGGAGCACTGAGCTTGAAGGAGTCACAGGAACTGGCCCAACATGCA
  GCAGAAATAGGAGCTGATGGCATCGCTGTCATTGCACCGTTCTTCCTCAAGCCATGGACC
  AAAGATATCCTGATTAATTTCCTAAAGGAAGTGGCTGCTGCCGCCCCTGCCCTGCCATTT
  TATTACTATCACATTCCTGCCTTGACAGGGGTAAAGATTCGTGCTGAGGAGTTGTTGGAT
  GGGATTCTGGATAAGATCCCCACCTTCCAAGGGCTGAAATTCAGTGATACAGATCTCTTA
  GACTTCGGGCAATGTGTTGATCAGAATCGCCAGCAACAGTTTGCTTTCCTTTTTGGGGTG
  GATGAGCAACTGTTGAGTGCTCTGGTGATGGGAGCAACTGGAGCAGTGGGCAGTACCTAT
  AACTACCTGGGAAAAAAGACAAACCAGATGTTGGAGGCTTTTGAACAAAAGGACTTCTCT
  TTAGCCCTGAACTATCAGTTTTGTATCCAGAGATTTATCAACTTTGTTGTCAAACTAGGT
  TTTGGAGTGTCACAGACCAAAGCCATCATGACTCTGGTCTCTGGGATTCCAATGGGCCCA
  CCCCGGCTTCCACTGCAGAAAGCCTCCAGGGAGTTTACTGATAGTGCTGAAGCTAAACTG
  AAGAGCCTGGATTTCCTTTCTTTCACTGATTTAAAGGATGGAAACTTGGAAGCTGGTAGC
  TAG
  
  Clone variation with respect to NM_030769.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_030769 unedited
  TAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCAAGAGCGGAGGCCTGGG
  TGAGCGCGGCCCGCGACGGCACGGGCTAGGACAGACGGACGCAGACGGCCCGACGACCTG
  GGCGTTCGGGCGGAGGGGCGCTGGGGCCTGACTCGCGGAAGGGGAAACTGAAGCAGAAAG
  AGGTTACTTAATGGGCTCAAGGTCACCTACCTGACCTACCAGCAGCTCAATGGCCTTCCC
  AAAGAAGAAACTTCAGGGTCTTGTGGCTGCAACCATCACGCCAATGACTGAGAATGGAGA
  AATCAACTTTTCAGTAATTGGTCAGTATGTGGATTATCTTGTGAAAGAACAGGGAGTGAA
  GAACATTTTTGTGAATGGCACAACAGGAGAAGGCCTGTCCCTGAGCGTCTCAGAGCGTCG
  CCAGGTTGCAGAGGAGTGGGTGACAAAAGGGAACGACAAGCTGGATCAGGTGATAATTCA
  CGTAGGAGCACTGAGCTTGAACGAGCTACAGGAACTGGCCCAACATGCACCATAAATACG
  AGCTGATGGCATCGCTGTCATTGCACCGTTCTTNCTCCAGCCATGGACCAAAGATATCCT
  GCATAATTCCCTAAAGGAACCGCCTGCTGCCGCCCCTTAACTGCCTTTATATTATTATAA
  CGTCCGTGCCCTTCGCCGCCCTACTGGCCCCCCCCCCCGGCCCCCCCCCCCCCCCCCGAT
  CCAATACCCCGCCCGTTGGCACCCCTATCACCATACCCCCCCCCGCCGCTTCCCCCTACC
  CTTGCTGGAAATTAAAATACGGTANTACCCCCCCCCCCCATGTTCGCCCGCCTCCGCCCC
  CACCACTATATCTCCTGACCAACACCTATCCCCTCTCGCTTGCCGCGAAAGCACCTGCGT
  CGCCACACCACTCCACCCCCCCTCCCGCCTCTGCTCTCTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_030769 unedited
  TGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCCTTTTGAAAAATTT
  TATTTTTAATTCAAAAAGATTAAAATAAGGAGCAAAACTGATATGAATTTCAAATACTCT
  TTCTCTGCCAATGATCAAAACAATTAGAGATGAAATGAAAACAAAATCTTAGGTAATTAA
  CAGACTGGATAGTCAAGTTTAAAGTCAGTAAGGGTTAACTGTCAATATACTTTGATATCA
  GAAAGCTTATATCAAGGTTTGTTAGAAGGGAGATTAAACAGTTGTTGATACATTACACAG
  AAAGAAAAGAGTAAAAGGAGGTATGGAGAGAAAGTTCTGAGTCCTTTGGGTCCTCCCCAA
  ATCTTCTATCACTAAACAAAAGTCACTAGTTGCAAGTGTGACAGGATAATAACATTAGCC
  CCTGGGCTCACTGCAGAAAGGAAGTCAGCCTTCGATTTCAATAATTCAGGGTCCTGCGGG
  AGAGACAGTCTTCTCAGTGGAGGAAGGGGAAGTTTGTGGTGCCCAGAGGAAGAGTCCTTT
  GGTTCTTTGAAACTTTGAGTTTTGAGTTTTCTGTATAGAAAAAAGTGGAGACTTAAATTC
  ATCTTTGGGGAAGACTTTTTCCTTTGTTATGAGAAAGCCGCCCTTTACTTAGTTATAGGA
  ACTGAAACAAGTCCTCACCTGACCCAGTGTTTTTAAAGAGCGAAAAGTGGTGACATCGAA
  ATGAATTCAAAAGCATCCATTCTAGAGTTAGACTGAGCAGCCAAATGAGCTGGTGTATTT
  TGAGTTGTATACAGTTGAGACATGTTACAGTTTGGTATTAAAGTGCCTGCCTAAATGCAG
  TAGCTCAGAAGTTTCCTAGATTCCAATGAATATTTATTNAAATGAGACATATATAGTGGG
  TTAAAATTANACACTTTNAAATAGGANAGGGACCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_030769
• Insert Size: 2500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030769.1, NP_110396.1
• RefSeq Size: 1552 bp
• RefSeq ORF: 963 bp
• Locus ID: 80896
• UniProt ID: Q9BXD5
• Cytogenetics: 1q25.3
• Domains: DHDPS
• Protein Pathways: Amino sugar and nucleotide sugar metabolism
• Gene Summary: This gene encodes a member of the N-acetylneuraminate lyase sub-family of (beta/alpha)(8)-barrel enzymes. N-acetylneuraminate lyases regulate cellular concentrations of N-acetyl-neuraminic acid (sialic acid) by mediating the reversible conversion of sialic acid into N-acetylmannosamine and pyruvate. A pseudogene of this gene is located on the short arm of chromosome 2. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011]
  Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.