5 HT1F (HTR1F) (NM_000866) Human Untagged Clone

CAT#: SC108583

HTR1F (untagged)-Human 5-hydroxytryptamine (serotonin) receptor 1F (HTR1F)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: 5 HT1F
• Synonyms: 5-HT-1F; 5-HT1F; 5HT6; HTR1EL; MR77
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000866, RT-PCR generated
  TGGATTTCTTAAATTCATCTGATCAAAACTTGACCTCAGAGGAACTGTTAAACAGAATGC
  CATCCAAAATTCTGGTGTCCCTCACTCTGTCTGGGCTGGCACTGATGACAACAACTATCA
  ACTCCCTTGTGATCGCTGCAATTATTGTGACCCGGAAGCTGCACCATCCAGCCAATTATT
  TAATTTGTTCCCTTGCAGTCACAGATTTTCTTGTGGCTGTCCTGGTGATGCCCTTCAGCA
  TTGTGTATATTGTGAGAGAGAGCTGGATTATGGGGCAAGTGGTCTGTGACATTTGGCTGA
  GTGTTGACATTACCTGCTGCACGTGCTCCATCTTGCATCTCTCAGCTATAGCTTTGGATC
  GGTATCGAGCAATCACAGATGCTGTTGAGTATGCCAGGAAAAGGACTCCAAAGCATGCTG
  GCATTATGATTACAATAGTTTGGATTATATCTGTTTTTATCTCTATGCCTCCTCTATTCT
  GGAGGCACCAAGGAACTAGCAGAGATGATGAATGCATCATCAAGCACGACCACATTGTTT
  CCACCATTTACTCAACATTTGGAGCTTTCTACATCCCACTGGCATTGATTTTGATCCTTT
  ACTACAAAATATATAGAGCAGCAAAGACATTATACCACAAGAGACAAGCAAGTAGGATTG
  CAAAGGAGGAGGTGAATGGCCAAGTCCTTTTGGAGAGTGGTGAGAAAAGCACTAAATCAG
  TTTCCACATCCTATGTACTAGAAAAGTCTTTATCTGACCCATCAACAGACTTTGATAAAA
  TTCATAGCACAGTGAGAAGTCTCAGGTCTGAATTCAAGCATGAGAAATCTTGGAGAAGGC
  AAAAGATCTCAGGTACAAGAGAACGGAAAGCAGCCACTACCCTGGGATTAATCTTGGGTG
  CATTTGTAATATGTTGGCTTCCTTTTTTTGTAAAAGAATTAGTTGTTAATGTCTGTGACA
  AATGTAAAATTTCTGAAGAAATGTCCAATTTTTTGGCATGGCTTGGGTATCTCAATTCCC
  TTATAAATCCACTGATTTACACAATCTTTAATGAAGACTTCAAGAAAGCATTCCAAAAGC
  TTGTGCGATGTCGATGTTAG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000866 unedited
  CCCGCCATTCGGGAATTGTAAACGATTCACTATAGGGCGGCCGCCAGTGTGATGGATATC
  TGCAGAATTCGGCTTATGGATTTCTTAAATTCATCTGATCAAAACTTGACCTCAGAGGAA
  CTGTTAAACAGAATGCCATCCAAAATTCTGGTGTCCCTCACTCTGTCTGGGCTGGCACTG
  ATGACAACAACTATCAACTCCCTTGTGATCGCTGCAATTATTGTGACCCGGAAGCTGCAC
  CATCCAGCCAATTATTTAATTTGTTCCCTTGCAGTCACAGATTTTCTTGTGGCTGTCCTG
  GTGATGCCCTTCAGCATTGTGTATATTGTGAGAGAGAGCTGGATTATGGGGCAAGTGGTC
  TGTGACATTTGGCTGAGTGTTGACATTACCTGCTGCACGTGCTCCATCTTGCATCTCTCA
  GCTATAGCTTTGGATCGGTATCGAGCAATCACAGATGCTGTTGAGTATGCCAGGAAAAGG
  ACTCCAAAGCATGCTGGCATTATGATTACAATAGTTTGGATTATATCTGTTTTTATCTCT
  ATGCCTCCTCTATTCTGGAGGCACCAAGGAACTAGCAGAGATGATGAATGCATCATCAAG
  CACGACCACATTGTTTCCACCATTTACTCAACATTTGGAGCTTTCTACATCCCACTGGCA
  TTGATTTTGATCCTTTACTACAAAATATATAGAGCAGCAAAGACATTATACCACAAGAGA
  CAAGCAAGTAGGATTGCAAAGGAGGAGGTGAATGGCCAAGTCCTTTTGGAGAGTGGTGAG
  AAAAGCACTAAATCAGTTTCCACATCCTATGTACTAGAAAAGTCTNTATCTGACCCATCA
  ACAGACTNTGATAAAATTCATAGCACAGTGAGAAGTCTCAAGTCTGAATTCAGCATGAGA
  N
  
• Restriction Sites: Please inquire
• ACCN: NM_000866
• Insert Size: 1100 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000866.1, NP_000857.1
• RefSeq Size: 1101 bp
• RefSeq ORF: 1101 bp
• Locus ID: 3355
• UniProt ID: P30939
• Cytogenetics: 3p11.2-p11.1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• Gene Summary: G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various alkaloids and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase activity.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: Variants 1 through 4 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.