DFFB (NM_004402) Human Untagged Clone

CAT#: SC107911

DFFB (untagged)-Human DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase) (DFFB)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DFFB
• Synonyms: CAD; CPAN; DFF-40; DFF2; DFF40
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC107911 sequence for NM_004402 edited (data generated by NextGen Sequencing)
  ATGCTCCAGAAGCCCAAGAGCGTGAAGCTGCGGGCCCTGCGCAGCCCGAGGAAGTTCGGC
  GTGGCTGGCCGGAGCTGCCAGGAGGTGCTGCGCAAGGGCTGTCTCCGCTTCCAGCTCCCT
  GAGCGCGGTTCCCGGCTGTGCCTGTACGAGGATGGCACGGAGCTGACGGAAGATTACTTC
  CCCAGTGTTCCCGACAACGCCGAGCTGGTGCTGCTCACCTTGGGCCAGGCCTGGCAGGGC
  TATGTGAGCGACATCAGGCGCTTCCTCAGTGCATTTCACGAGCCACAGGTGGGGCTCATC
  CAGGCCGCCCAGCAGCTGCTGTGTGATGAGCAGGCCCCACAGAGGCAGAGGCTGCTGGCT
  GACCTCCTGCACAACGTCAGCCAGAACATCGCGGCCGAGACCCGGGCTGAGGACCCGCCG
  TGGTTTGAAGGCTTGGAGTCCCGATTTCAGAGCAAGTCTGGCTATCTGAGATACAGCTGT
  GAGAGCCGGATCCGGAGTTACCTGAGGGAGGTGAGCTCCTACCCCTCCACGGTGGGTGCG
  GAGGCTCAGGAGGAATTCCTGCGGGTCCTCGGCTCCATGTGCCAGAAGCTCCGGTCCATG
  CAGTACAATGGCAGCTACTTCGACAGAGGAGCCAAGGGCGGCAGCCGCCTCTGCACACCG
  GAAGGCTGGTTCTCCTGCCAGGGTCCCTTTGACATGGACAGCTGCTTATCAAGACACTCC
  ATCAACCCCTACAGTAACAGGGAGAGCAGGATCCTCTTCAGCACCTGGAACCTGGATCGC
  ATAATAGAAAAGAAACGCACCATCATTCCTACACTGGTGGAAGCAATTAAGGAACAAGAT
  GGAAGAGAAGTGGACTGGGAGTATTTTTATGGCCTGCTTTTTACCTCAGAGAACCTAAAA
  CTAGTGCACATTGTCTGCCATAAGAAAACCACCCACAAGCTCAACTGTGACCCAAGCAGA
  ATCTACAAACCCCAGACAAGGTTGAAGCGGAAGCAGCCTGTGCGGAAACGCCAGTGA
  
  Clone variation with respect to NM_004402.2
  587 g=>a;779 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004402 unedited
  AAAAAAAGGGGANNNANAANNAAAAACACCCCCCCGGTTCACAATTGTATACGACTCATA
  TAGGCGGCCGCGNAATTCGCACGAGGCCAGCTTGCAGAGCTCACCAGGTGCAGACCCCTG
  CGGCCAGGGCGAGGACGGATCTGAGCAGCTGGGCAGCAGGTGCCACCGCCTGTGGGACCC
  AGAGGGCTTGAGGACATCTGCAATGCTCCAGAAGCCCAAGAGCGTGAAGCTGCGGGCCCT
  GCGCAGCCCGAGGAAGTTCGGCGTGGCTGGCCGGAGCTGCCAGGAGGTGCTGCGCAAGGG
  CTGTCTCCGCTTCCAGCTCCCTGAGCGCGGTTCCCGGCTGTGCCTGTACGAGGATGGCAC
  GGAGCTGACGGAAGATTACTTCCCCAGTGTTCCCGACAACGCCGAGCTGGTGCTGCTCAC
  CTTGGGCCAGGCCTGGCAGGGCTATGTGAGCGACATCAGGCGCTTCCTCAGTGCATTTCA
  CGAGCCACAGGTGGGGCTCATCCAGGCCGCCCAGCAGCTGCTGTGTGATGAGCAGGCCCC
  ACAGAGGCAGAGGCTGCTGGCTGACCTCCTGCACAACGTCAGCCAGAACATCGCGGCCGA
  GACCCGGGCTGAGGACCCGCCGTGGTTTGAAGGCTTGGAGTCCCGATTTCAGAGCAAGTC
  TGGCTATCTGAGATACAGCTGTGAGAGCCCNATCCGGAGTTACCTGNAGGAGGTGAGCTC
  CTACCCCTCCCACGTGGGTGCGGAGGCTCCAGAGGAATTCCTGCCGCTCCTCCGCTCCAT
  GGGCCANAAGCTTCCGTCCATGCAGTACAATGGCAGCTACTTCCACAGAGGAGCCAAGGC
  CGGCAGCCGCCTTTGCACACCGGAAGGGCTGTTCTCCCTGCCGGGTCCCTTTGACAGGAA
  CAGCTGCTTAAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004402 unedited
  NNTTTCTGTGNACCGCGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTTTATTTT
  TGAAAAATCTTTATTGCTTTTCCTGATTGTACAACAAATGTAAAAATTCAAATATTACAA
  AATGACTTAATTTAAAAAGTACATTTTAACATGTTATAAAATAAATGCCCATTTCAAAAA
  ATTCAAGTATCATAAAATACATGACGTAGAAAATGAGTCCTCTGTAATCTAGCCCAACCC
  CCGTATCCCCCAGGATTACTGCTGTTCAGATCCGCGTGTTTCCTTCCAGACCGTCTCGTG
  GTGNCNNNNNACCCNCCCGGNCCNCCCAACCCCCCCAACCAACGGGGCCTTGGACCATGA
  AGCCTTCCATATTGAATCCCGNAGNATGAAATAGTTAACCGCTGAACCCAACGGAAGCCA
  AGGGTTTCAANCTGCCCGGATGCCCCCTGGAAACAAGCTTCCCAGTTTTCCTTAACACCA
  GAAAACCCCTTTTTCCCTTCAATAAAAAAACCCTTGGAAAAACCTGGGGGAACACCAAAT
  TTTTTTGGCCCCTTTTGGTAAAATGGTATTCAAGGGTTGGAAATCCCTTGAAAAATAAAA
  TGGGATACCCCTTAACCCATTTTTTTTGGGCCCCTTAAAAAAAAAAAACAAGAGTTTTCC
  CCCGGGGGTTCAAAACGGGGAAAAAAACTTTGGTTTTCAAAAAAAAAACAATTTGGGGGG
  GGGGGGGTTTTCCCCCTTTTTTTTCTCTGTGGCCCCTTTTTTTAATAAAACAAATTTTTC
  AACCCGGGGGGGTCACGAGGGGGCCCCCACGGGGGGGGTTTTAAAAGCCCCCCCGGTGAT
  AAACCCCCCCCCACCACCCTC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004402
• Insert Size: 3340 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004402.2, NP_004393.1
• RefSeq Size: 3034 bp
• RefSeq ORF: 1017 bp
• Locus ID: 1677
• UniProt ID: O76075
• Cytogenetics: 1p36.32
• Domains: CAD
• Protein Families: Druggable Genome
• Protein Pathways: Apoptosis
• Gene Summary: Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of some of these variants has not been determined. [provided by RefSeq, Sep 2013]
  Transcript Variant: This variant (2) contains two consecutive alternate splice junctions compared to variant 1. The resulting isoform (2) has the same N- and C-termini but lacks an internal segment compared to isoform 1.