Thrombin Receptor (F2R) (BC016059) Human Untagged Clone

CAT#: SC106127

(untagged)-Homo sapiens, Similar to coagulation factor II (thrombin) receptor, clone IMAGE:4849569

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Thrombin Receptor
• Synonyms: CF2R; HTR; PAR-1; PAR1; TR
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for BC016059, the custom clone sequence may differ by one or more nucleotides
  
  
  CTGCCGCGAAGACCGGCTCCCCGACCCGCAGAAGTCAGGAGAGAGGGTGAAGCGGAGCAGCCCGAGGCGG
  GGCAGCCTCCCGGAGCAGCGCCGCGCAGAGCCCGGGACAATGGGGCCGCGGCGGCTGCTGCTGGTGGCCG
  CCTGCTTCAGTCTGTGCGGCCCGCTGTTGTCTGCCCGCACCCGGGCCCGCAGGCCAGTCACGTTTAGGTG
  GGGCGTGCCACCCCCTTCGCGGGCCCAGCCGATGCCCCTTTGGACTCGATCTTGGAGGGTGCAGCCCGCC
  TGCCACGGGGTGTTGGATATGGAGGAGGATGGAGCGGAAGCCCCCTGGGGGAGCCTGCAGTCCTGCGTTG
  CACTTGTCATTGTGTTTCTCCCAGGACCACCCCCAAAAAGAAAAGCTCTCACGTTGCTCCACCAATAAAC
  GTTTCGATCTTTAAAATATAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC016059 unedited
  GGTTCGGATTTTGTAATACGACTTCACTATAGGGCGGCCGCGATTCGGCACGAGGCTTGG
  ACCCTGATCTTACCCGTGGGCACCCTGCGCTCTGCCTGCCGCGAAGACCGGCTCCCCGAC
  CCGCAGAAGTCAGGAGAGAGGGTGAAGCGGAGCAGCCCGAGGCGGGGCAGCCTCCCGGAG
  CAGCGCCGCGCAGAGCCCGGGACAATGGGGCCGCGGCGGCTGCTGCTGGTGGCCGCCTGC
  TTCAGTCTGTGCGGCCCGCTGTTGTCTGCCCGCACCCGGGCCCGCAGGCCAGAATCAAAA
  GCAACAAATGCCACCTTAGATCCCCGGTCATTTCTTCTCAGGAACCCCAATGATAAATAT
  GAACCATTTTGGGAGGATGAGGAGAAAAATGAAAGTGGGTTAACTGAATACAGATTAGTC
  TCCATCAATAAAAGCAGTCCTCTTCAAAAACAACTTCCTGCATTCATCTCAGAAGATGCC
  TCCGGATATTTGACCAGCTCCTGGCTGACACTCTTTGTCCCATCTGTGTACACCGGAGTG
  TTTGTAGTCAGCCTCCCACTAAACATCATGGCCATCGTTGTGTTCATCCTGAAAATGAAG
  GTCAAGAAGCCGGCGGTGGTGTACATGCTGCACCTGGCCACGGCAGATGTGCTGTTTGTG
  TCTGTGCTCCCCTTTAAGATCAGCTATTACTTTTCCGGCAGTGATTGGCAGTTTGGGTCT
  GAATTGTGTCGCTTCGTCACTGCAGCATTTTACTGGTACATGTACGCCTCTATCTTGCTC
  ATGACAGTCATAAGCATTGACCGGNTTCTGGCTGTGGTGTATCCCATGCAGTCCCTCTCC
  TGGCGTACTCTGGGAAGGGGCTNCNTCACTNGTCTGGCCATCTGGGCTTTGCCATCGCAG
  GGGTAGTGCCCTCTGCTCTCAGGAGCAACCATNNCAGTGCCGGNCTCACAT
  
• Restriction Sites: NotI-NotI
• ACCN: BC016059
• Insert Size: 3800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC016059.1
• RefSeq Size: 496 bp
• RefSeq ORF: 496 bp
• Locus ID: 2149
• Cytogenetics: 5q13.3
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Calcium signaling pathway, Complement and coagulation cascades, Endocytosis, Neuroactive ligand-receptor interaction, Regulation of actin cytoskeleton
• Gene Summary: Coagulation factor II receptor is a 7-transmembrane receptor involved in the regulation of thrombotic response. Proteolytic cleavage leads to the activation of the receptor. F2R is a G-protein coupled receptor family member. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]