ATP5H (BC032245) Human Untagged Clone

CAT#: SC104218

ATP5H (untagged)-Homo sapiens, ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d, clone MGC:39974 IMAGE:5167128, complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ATP5H
• Synonyms: ATP5JD; ATPQ; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d; ATP synthase, H+ transporting, mitochondrial F1F0, subunit d; ATP synthase D chain, mitochondrial; My032 protein
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for BC032245 edited
  ATGGCTGGGCGAAAACTTGCTCTAAAAACCATTGACTGGGTAGCTTTTGCAGAGATCATA
  CCCCAGAACCAAAAGGCCATTGCTAGTTCCCTGAAATCCTGGAATGAGACCCTCACCTCC
  AGGTTGGCTGCTTTACCTGAGAATCCACCAGCTATCGACTGGGCTTACTACAAGGCCAAT
  GTGGCCAAGGCTGGCTTGGTGGATGACTTTGAGAAGAAGTTTAATGCGCTGAAGGTTCCC
  GTGCCAGAGGATAAATATACTGCCCAGGTGGATGCCGAAGAAAAAGAAGATGTGAAATCT
  TGTGCTGAGTGGGTGTCTCTCTCAAAGGCCAGGATTGTAGAATATGAAAAAGAGATGGAG
  AAGATGAAGAACTTAATTCCATTTGATCAGATGACCATTGAGGACTTGAATGAAGCTTTC
  CCAGAAACCAAATTAGACAAGAAAAAGTATCCCTATTGGCCTCACCAACCAATTGAGAAT
  TTATAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC032245 unedited
  GGGTTCAAAATTTGTAATACGACTCACTCATAGGGCGGCCGCGAATTCGCACCGCCGGGT
  CGGTGAAGGATCCCAAAATGGCTGGGCGAAAACTTGCTCTAAAAACCATTGACTGGGTAG
  CTTTTGCAGAGATCATACCCCAGAACCAAAAGGCCATTGCTAGTTCCCTGAAATCCTGGA
  ATGAGACCCTCACCTCCAGGTTGGCTGCTTTACCTGAGAATCCACCAGCTATCGACTGGG
  CTTACTACAAGGCCAATGTGGCCAAGGCTGGCTTGGTGGATGACTTTGAGAAGAAGTTTA
  ATGCGCTGAAGGTTCCCGTGCCAGAGGATAAATATACTGCCCAGGTGGATGCCGAAGAAA
  AAGAAGATGTGAAATCTTGTGCTGAGTGGGTGTCTCTCTCAAAGGCCAGGATTGTAGAAT
  ATGAAAAAGAGATGGAGAAGATGAAGAACTTAATTCCATTTGATCAGATGACCATTGAGG
  ACTTGAATGAAGCTTTCCCAGAAACCAAATTAGACAAGAAAAAGTATCCCTATTGGCCTC
  ACCAACCAATTGAGAATTTATAAAATTGAGTCCAGGAGGAAGCTCTGGCCCTTGTATTAC
  ACATTCTGGACATTAAAAATAATAATTATACAGTTCAAAAAAAAAAAAAAAAAAACTCGA
  CTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCCGGTTGCATTCCCTG
  TGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTCCACTTCAGTGCCCACCAGCCTT
  GTCCTATAAAAATAAGTNGCATCATTTTGTCTGACTAAGTGTCCCTCTATATACTATCGG
  GTGAAGGGCGTGGATTGGACCAGGGGCAATTTGGAAAAACAACTTTTGGCCCGCGGGGTC
  ATTGGGAACCACC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for BC032245 unedited
  GGTACTACTATGNNACCGCGGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTTGA
  ACTGTATAATTATTATTTTTAATGTCCAGAATGTGTAATACAAGGGCCAGAGCTTCCTCC
  TGGACTCAATTTTATAAATTCTCAATTGGTTGGTGAGGCCAATAGGGATACTTTTTCTTG
  TCTAATTTGGTTTCTGGGAAAGCTTCATTCAAGTCCTCAATGGTCATCTGATCAAATGGA
  ATTAAGTTCTTCATCTTCTCCATCTCTTTTTCATATTCTACAATCCTGGCCTTTGAGAGA
  GACACCCACTCAGCACAAGATTTCACATCTTCTTTTTCTTCGGCATCCACCTGGGCAGTA
  TATTTATCCTCTGGCACGGGAACCTTCAGCGCATTAAACTTCTTCTCAAAGTCATCCACC
  AAGCCAGCCTTGGCCACATTGGCCTTGTAGTAAGCCCAGTCGATAGCTGGTGGATTCTCA
  GGTAAAGCAGCCAACCTGGAGGTGAGGGTCTCATTCCAGGATTTCAGGGAACTAGCAATG
  GCCCTTTGGTTCTGGGGTATGATCTCTGCAAAAGCTCCCCCATCAATGGTTTTTAGAGCA
  AAGTTTTCCCCACCCCTTTGGGATCCTTCCCCGACCCTGGCTGTGGCCAAATTCTCGGGC
  CGCCCTTAGTGAGTCCTATTAAAAAAATTCTGACCGTTCATTAAACAAGCTTTGGTTATT
  TAAACCTCCACCGGTCCCCCCCTCCCGCCCTTTTTGGTTACCGGGGGGGGTTTTTCCAAA
  TTTTGGAAAGTCCCGTTGATTTTGGGGCCCAAAAAACCCCCTTTGACGTCAAGGGGGGGG
  AAACTGGAAATCCCCCTGGGTAAAACCCCTTTCCCCCCCCTTGTGGGTCTGCCAAAA
  
• Restriction Sites: NotI-NotI
• ACCN: BC032245
• Insert Size: 420 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC032245.1, AAH32245.1
• RefSeq Size: 592 bp
• RefSeq ORF: 414 bp
• Locus ID: 10476
• Cytogenetics: 17q25.1
• Protein Pathways: Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease
• Gene Summary: Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the d subunit of the Fo complex. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. In addition, three pseudogenes are located on chromosomes 9, 12 and 15. [provided by RefSeq, Jun 2010]