GPR116 (ADGRF5) (NM_015234) Human Untagged Clone

CAT#: SC100690

GPR116 (untagged)-Human G protein-coupled receptor 116 (GPR116), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GPR116
• Synonyms: GPR116; KPG_001
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC100690 sequence for NM_015234 edited (data generated by NextGen Sequencing)
  ATGAAATCCCCAAGGAGAACCACTTTGTGCCTCATGTTTATTGTGATTTATTCTTCCAAA
  GCTGCACTGAACTGGAATTACGAGTCTACTATTCATCCTTTGAGTCTTCATGAACATGAA
  CCAGCTGGTGAAGAGGCACTGAGGCAAAAACGAGCCGTTGCCACAAAAAGTCCTACGGCT
  GAAGAATACACTGTTAATATTGAGATCAGTTTTGAAAATGCATCCTTCCTGGATCCTATC
  AAAGCCTACTTGAACAGCCTCAGTTTTCCAATTCATGGGAATAACACTGACCAAATTACT
  GACATTTTGAGCATAAATGTGACAACAGTCTGCAGACCTGCTGGAAATGAAATCTGGTGC
  TCCTGCGAGACAGGTTATGGGTGGCCTCGGGAAAGGTGTCTTCACAATCTCATTTGTCAA
  GAGCGTGACGTCTTCCTCCCAGGGCACCATTGCAGTTGCCTTAAAGAACTGCCTCCCAAT
  GGACCTTTTTGCCTGCTTCAGGAAGATGTTACCCTGAACATGAGAGTCAGACTAAATGTA
  GGCTTTCAAGAAGACCTCATGAACACTTCCTCCGCCCTCTATAGGTCCTACAAGACCGAC
  TTGGAAACAGCGTTCCGGAAGGGTTACGGAATTTTACCAGGCTTCAAGGGCGTGACTGTG
  ACAGGGTTCAAGTCTGGAAGTGTGGTTGTGACATATGAAGTCAAGACTACACCACCATCA
  CTTGAGTTAATACATAAAGCCAATGAACAAGTTGTACAGAGCCTCAATCAGACCTACAAA
  ATGGACTACAACTCCTTTCAAGCAGTTACTATCAATGAAAGCAATTTCTTTGTCACACCA
  GAAATCATCTTTGAAGGGGACACAGTCAGTCTGGTGTGTGAAAAGGAAGTTTTGTCCTCC
  AATGTGTCTTGGCGCTATGAAGAACAGCAGTTGGAAATCCAGAACAGCAGCAGATTCTCG
  ATTTACACCGCACTTTTCAACAACATGACTTCGGTGTCCAAGCTCACCATCCACAACATC
  ACTCCAGGTGATGCAGGTGAATATGTTTGCAAACTGATATTAGACATTTTTGAATATGAG
  TGCAAGAAGAAAATAGATGTTATGCCCATCCAAATTTTGGCAAATGAAGAAATGAAGGTG
  ATGTGCGACAACAATCCTGTATCTTTGAACTGCTGCAGTCAGGGTAATGTTAATTGGAGC
  AAAGTAGAATGGAAGCAGGAAGGAAAAATAAATATTCCAGGAACCCCTGAGACAGACATA
  GATTCTAGCTGCAGCAGATACACCCTCAAGGCTGATGGAACCCAGTGCCCAAGCGGGTCG
  TCTGGAACAACAGTCATCTACACTTGTGAGTTCATCAGTGCCTATGGAGCCAGAGGCAGT
  GCAAACATAAAAGTGACATTCATCTCTGTGGCCAATCTAACAATAACCCCGGACCCAATT
  TCTGTTTCTGAGGGACAAAACTTTTCTATAAAATGCATCAGTGATGTGAGTAACTATGAT
  GAGGTTTATTGGAACACTTCTGCTGGAATTAAAATATACCAAAGATTTTATACCACGAGG
  AGGTATCTTGATGGAGCAGAATCAGTACTGACAGTCAAGACCTCGACCAGGGAGTGGAAT
  GGAACCTATCACTGCATATTTAGATATAAGAATTCATACAGTATTGCAACCAAAGACGTC
  ATTGTTCACCCGCTGCCTCTAAAGCTGAACATCATGGTTGATCCTTTGGAAGCTACTGTT
  TCATGCAGTGGTTCCCATCACATCAAGTGCTGCATAGAGGAGGATGGAGACTACAAAGTT
  ACTTTCCATATGGGTTCCTCATCCCTTCCTGCTGCAAAAGAAGTTAACAAAAAACAAGTG
  TGCTACAAACACAATTTCAATGCAAGCTCAGTTTCCTGGTGTTCAAAAACTGTTGATGTG
  TGTTGTCACTTTACCAATGCTGCTAATAATTCAGTTTGGAGCCCATCTATGAAGCTGAAT
  CTGGTTCCTGGGGAAAACATCACATGCCAGGATCCCGTAATAGGTGTCGGAGAGCCGGGG
  AAAGTCATCCAGAAGCTATGCCGGTTCTCAAACGTTCCCAGCAGCCCTGAGAGTCCCATT
  GGCGGGACCATCACTTACAAATGTGTAGGCTCCCAGTGGGAGGAGAAGAGAAATGACTGC
  ATCTCTGCCCCAATAAACAGTCTGCTCCAGATGGCTAAGGCTTTGATCAAGAGCCCCTCT
  CAGGATGAGATGCTCCCTACATACCTGAAGGATCTTTCTATTAGCATAGACAAAGCGGAA
  CATGAAATCAGCTCTTCTCCTGGGAGTCTGGGAGCCATTATTAACATCCTTGATCTGCTC
  TCAACAGTTCCAACCCAAGTAAATTCAGAAATGATGACGCACGTGCTCTCTACGGTTAAT
  GTCATCCTTGGSAAGCCCGTCTTGAACACCTGGAAGGTTTTACAASAGCAAYGGACCAAK
  CAGAGTTCACAGCTACTACATTCAGTGGAAAGATTTTCCCAAGCATTACAGTCAGGAGAT
  AGCCCTCCTTTGTCCTTCTCCCAAACTAATGTGCAGATGAGCAGCAYGGTAATCAWGTCC
  AGCCACCCARAAACCTATCAACAGAGGTTTGTTTTCCCATACTTTGACCTCTGGGGCAAT
  GTGGTCATTGACAAGAGCTATCTAGAAAACTTGCAGTCGGATTCGTCTATTGTCACCATG
  GCTTTCCCAACTCTCCAAGCCATCCTTGCYCAGGRTATCCAGGAAAATAAYTTTGCAGAG
  AGCTTAGTGATGACAACCACTGTCAGCCACAATACGACTATGCCATTCAGGATTTCAATG
  ACTTTTAAGAACAATAGCCCTTCAGGCGGCGAAACGAAGTGTGTCTTCTGGAACTTCAGG
  CTTGCCAACAACACAGGGGGGTGGGACAGCAGTGGGTGCTATGTTGAAGAAGGTGATGGG
  GACAATGTCACCTGTATCTGTGACCACCTAACATCATTCTCCATCCTCATGTCCCCTGAC
  TCCCCAGATCCTAGTTCTCTCCTGGGAATACTMCTGGATATTATTTCTTATGTTGGGGTG
  GGCTTTTCCATCTTSAGCTTGGCRGCCTGTCTAGTTGTGGAARCTGTGGTGTGGAAATCG
  GTGACCAAGAATCGGACTTCTTATATGCGCCACACCTGCATAGTGAATRTYGCTGCCTCC
  CTTCTGGTCGCCAACACCTGGTTCATTGTGGTCGCTGCCATCCAGGACAATCRCTACATA
  CTCTGCAAGACAGCCTGTGTGGCTGCCACCTTCTTCATCCACTTCTTCTACCTCAGCRTC
  TTCTTCTGGATGCTGACACTGGGCCTCATGYTGTTCTATCGCCTGGTTTTCATTCTGCAY
  GAAACAAGCAGGTCCACTCAGAAAGCCATTGCCTTCTGTCTTGGCTAYGGCTGCCCACTT
  GCCATCTCGGTCATCAYGCTGGGAGCCACCCAGCCCYGGGAAGTCTATAYGAGGAAGAAT
  GTCTGTTGGCTCAACTGGGAGGACACCAAGGCCCTGCTGGCTTTYGCCATCCCAGCACTG
  ATCATTGTGGTGGTGAACATAACCATCACTATTGTGGTCATCACCAAGATCCTGAGGCCT
  TCCATTGGAGACAAGCCATGCAAGCAGGAGAAGAGCAGCCTGTTTCAGATCAGCAAGAGC
  ATTGGGGTCCTCACRCCACTCTTGGGCCTCACTTGGGGTTTTGGTCTCACCACTGTGTTC
  CCAGGGACCAACCTTGTGTTCCATATCATATTTGCCATCCTCAATGTCTTCCAGGGATTA
  TTCATTTTACTCTTTGGATGCCTCTGGGATCTGAAGGTACAGGAAGCTTTGCTGAATAAG
  TTTTCATTGTCGAGATGGTCTTCACAGCACTCAAAGTCAACATCCCTGGGTTCATCCACA
  CCTGTGTTTTCTATGAGTTCTCCAATATCAAGGAGATTTAACAATTTGTTTGGTAAAACA
  GGAACGTATAATGTTTCCACCCCAGAAGCAACCAGCTCATCCCTGGAAAACTCATCCAGT
  GCTTCTTCGTTGCTCAACTAA
  
  Clone variation with respect to NM_015234.4
  300 c=>t;1811 c=>t;1956 c=>t;2412 c=>s;2446 c=>s;2452 t=>y;2460 t=>k;2514 g=>a;2567 t=>y;2576 a=>w;2590 g=>r;2730 c=>y;2735 a=>r;2751 c=>y;2796 a=>g;2925 a=>t;3033 c=>m;3075 g=>s;3084 a=>r;3103 g=>r;3132 c=>t;3169 a=>r;3171 c=>y;3233 g=>r;3298 g=>r;3331 c=>y;3360 t=>y;3408 t=>y;3437 c=>y;3457 c=>y;3470 c=>y;3525 c=>y;3675 a=>r
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_015234 unedited
  NGGTGACAAATTTTGTATACGACTCATATAGGCGGCCGCGAAATTCGCACGAGGCACCTC
  TGCTCCCGACAGCCTGGGAACCCGCAAGATCCCCAGCATTTGAAGTCTGGTCTTGTGAAA
  CCCCACCCTCCTCTGGCTGTGTGATTGAATGGGATGCCCTCGAGGTTCACCTCACCTGAG
  AGGGTTTTGGGCAGATCAGCAGTAAGGTGTTAAATTTTAGAAGCCTGAAAACTCCAGAAG
  AGAAAGGCCAACCAACTCAAACTTGAAGACATGAAATCCCCAAGGAGAACCACTTTGTGC
  CTCATGTTTATTGTGATTTATTCTTCCAAAGCTGCACTGAACTGGAATTACGAGTCTACT
  ATTCATCCTTTGAGTCTTCATGAACATGAACCAGCTGGTGAAGAGGCACTGAGGCAAAAA
  CGAGCCGTTGCCACAAAAAGTCCTACGGCTGAAGAATACACTGTTAATATTGAGATCAGT
  TTTGAAAATGCATCCTTCCTGGATCCTATCAAAGCCTACTTGAACAGCCTCAGTTTTCCA
  ATTCATGGGAATAACACTGACCAAATTACTGACATTTTGAGCATAAATGTGACAACAGTC
  TGCAGACCTGCTGGAAATGAAATCTGGTGCTCCTGCGAGACAGGTTATGGGTGGCCTCGG
  GAAAGGTGTCTTCACAATCTCATTTGTCAAGAGCGTGACGTCTTCCTCCCAGGCACCATT
  GCAGTTGCCTTAAAGACTGCCTCCAATGGACCTTTNTGCCTGCTTCANGAAGATGTTACC
  CTGAACATGAGAGTCAGACTAAATGTANGCTTTCAAGAAGACCTCATGAACACTTCCTNN
  CGCCTCTATTAGTCCCTACAGACGACTTGNAACAGCGTNNCGAAGGGTACGGATTTTACC
  AGCTCAAGGCGTGACTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_015234
• Insert Size: 5850 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015234.3, NP_056049.3
• RefSeq Size: 5514 bp
• RefSeq ORF: 4041 bp
• Locus ID: 221395
• UniProt ID: Q8IZF2
• Cytogenetics: 6p12.3
• Domains: GPS, 7tm_2, ig, IGc2, IG
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Gene Summary: Receptor that plays a critical role in lung surfactant homeostasis. May play a role in controlling adipocyte function.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript. Variants 1 and 2 encode the same isoform.