KIAA0664 (CLUH) (NM_015229) Human Tagged ORF Clone

CAT#: RG220737

CLUH (tGFP-tagged) - Human KIAA0664 (KIAA0664)

Product Images

Circular map for RG220737

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: KIAA0664
• Synonyms: CLU1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG220737 representing NM_015229
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCTTAAACGGGGACTGCCCAGAGAGCCTGAAGAAGGAGGCGGCGGCGGCCGAGCCACCCAGGGAAA
  ATGGGCTTGACGAGGCCGGCCCGGGAGATGAGACCACCGGCCAGGAAGTCATTGTCATTCAGGACACGGG
  CTTTTCTGTGAAGATCCTCGCCCCTGGGATCGAGCCCTTCTCCCTGCAGGTGTCCCCCCAGGAGATGGTG
  CAGGAGATTCACCAGGTGCTCATGGACCGGGAGGACACGTGTCACCGCACCTGCTTCTCACTGCACCTGG
  ATGGCAACGTGCTGGACCACTTCTCGGAGCTGCGCAGCGTCGAGGGGCTGCAGGAGGGCTCTGTGCTGCG
  TGTGGTGGAAGAGCCGTACACGGTGCGTGAGGCCCGCATCCACGTGCGCCATGTCCGAGACCTGCTCAAG
  AGCCTGGACCCATCCGATGCCTTCAACGGGGTTGACTGCAACTCCTTGTCCTTCCTGAGTGTCTTCACCG
  ACGGCGACCTGGGAGACAGCGGGAAGCGGAAGAAGGGCTTGGAGATGGACCCCATCGACTGCACACCACC
  CGAGTACATCCTGCCAGGGAGCCGGGAGCGGCCACTGTGTCCCCTGCAGCCCCAAAACCGTGACTGGAAG
  CCCTTGCAGTGCCTGAAAGTACTCACCATGAGCGGATGGAACCCGCCCCCGGGGAACCGGAAGATGCACG
  GGGACCTCATGTACCTGTTTGTGATCACAGCCGAGGACCGGCAAGTCAGCATCACCGCGTCCACACGGGG
  CTTTTACCTGAATCAGTCCACAGCTTATCACTTCAACCCCAAGCCCGCCAGCCCCCGCTTCCTAAGCCAT
  TCCCTAGTGGAGCTGCTCAACCAGATCAGCCCGACCTTCAAGAAGAACTTCGCTGTGCTGCAGAAGAAAA
  GGGTCCAGCGCCACCCGTTCGAGAGGATCGCCACCCCATTCCAGGTGTACAGCTGGACAGCCCCCCAGGC
  GGAGCATGCCATGGATTGCGTGCGTGCAGAGGACGCCTACACCTCGAGGCTGGGCTATGAGGAGCACATT
  CCTGGACAGACCCGAGACTGGAATGAGGAGCTGCAGACGACGAGGGAGCTGCCTCGCAAGAACCTGCCTG
  AGCGGCTGCTCCGAGAAAGGGCCATATTCAAGGTGCACAGCGACTTCACCGCGGCAGCCACCAGGGGCGC
  CATGGCCGTCATTGACGGCAACGTGATGGCCATCAACCCCAGCGAGGAGACCAAGATGCAGATGTTCATC
  TGGAACAACATCTTCTTCAGCCTGGGCTTCGACGTCCGAGACCACTACAAGGACTTCGGGGGGGACGTGG
  CGGCCTACGTGGCGCCCACCAACGACCTGAATGGCGTCCGCACGTACAACGCGGTGGACGTGGAGGGGCT
  GTACACGCTGGGCACGGTGGTGGTGGATTACCGCGGCTACCGGGTCACGGCCCAGTCCATCATCCCCGGC
  ATCCTGGAGCGGGACCAGGAGCAGAGCGTCATCTACGGCTCCATCGACTTCGGCAAGACCGTGGTGTCAC
  ACCCGCGGTACCTGGAGCTGCTGGAGCGCACGAGTCGGCCCCTCAAGATCCTGCGGCACCAGGTGCTCAA
  CGACCGTGACGAGGAGGTGGAGCTCTGCTCCTCGGTCGAGTGCAAGGGCATCATTGGCAACGACGGGCGC
  CACTACATCCTCGACCTGCTGCGCACCTTCCCCCCGGACCTCAACTTCCTGCCCGTGCCTGGCGAGGAGC
  TGCCTGAGGAATGCGCCCGCGCCGGCTTCCCCCGCGCCCACCGGCACAAGCTCTGCTGCCTGCGCCAGGA
  GCTGGTGGACGCCTTCGTGGAGCACAGGTACCTCCTCTTTATGAAGCTGGCCGCCTTGCAGCTGATGCAG
  CAGAACGCCAGCCAGCTGGAGACCCCCTCCTCCCTGGAAAATGGTGGTCCTTCCTCCTTGGAGTCCAAGT
  CTGAGGATCCTCCAGGACAGGAGGCGGGAAGTGAGGAGGAGGGTAGCAGCGCCAGCGGCCTGGCCAAGGT
  GAAGGAGCTGGCAGAGACCATCGCCGCAGACGACGGCACAGACCCTCGGAGCCGGGAGGTGATCCGCAAC
  GCGTGCAAGGCGGTCGGCTCCATCAGCAGCACCGCCTTCGACATTCGCTTCAATCCTGACATCTTCTCAC
  CAGGGGTTCGTTTCCCTGAGTCCTGCCAGGATGAAGTTCGGGACCAGAAGCAGCTGCTGAAGGACGCGGC
  TGCCTTCCTGCTCTCCTGCCAGATCCCTGGCTTGGTGAAGGACTGCATGGAGCACGCGGTCCTGCCCGTG
  GACGGGGCAACGCTGGCAGAGGTGATGCGCCAGCGGGGCATCAACATGCGCTACCTGGGCAAGGTGCTGG
  AGCTGGTGCTGCGGAGCCCGGCCCGCCACCAGCTGGACCACGTCTTTAAAATCGGCATTGGAGAACTCAT
  CACCCGCTCGGCCAAGCACATCTTCAAGACGTACTTACAGGGAGTCGAGCTCTCCGGCCTCTCAGCCGCC
  ATCAGCCACTTCCTGAACTGCTTCCTGAGCTCCTACCCAAACCCCGTGGCCCACCTGCCCGCCGACGAGC
  TGGTCTCCAAGAAGCGGAATAAGAGGAGGAAAAACCGGCCCCCGGGGGCTGCAGATAACACAGCCTGGGC
  TGTCATGACCCCCCAGGAGCTCTGGAAGAACATCTGCCAGGAGGCCAAGAACTACTTTGACTTCGACCTC
  GAGTGTGAGACCGTGGACCAGGCTGTGGAGACCTACGGCCTGCAGAAGATAACGCTCCTGCGGGAGATCT
  CGCTGAAAACAGGGATCCAGGTCCTGCTGAAGGAGTACAGCTTCGACAGTCGCCACAAGCCCGCGTTCAC
  CGAGGAGGACGTGCTCAACATCTTCCCCGTGGTCAAGCACGTCAACCCCAAGGCCTCGGATGCCTTCCAT
  TTCTTCCAGAGCGGGCAGGCCAAAGTGCAGCAGGGCTTCCTGAAGGAGGGCTGTGAGCTCATCAATGAGG
  CCCTGAACCTGTTTAACAACGTCTACGGAGCCATGCACGTGGAGACCTGCGCCTGCCTGCGCCTCCTCGC
  CCGCCTCCACTACATCATGGGCGACTACGCAGAGGCCCTGAGTAACCAGCAGAAGGCGGTGCTGATGAGC
  GAGCGGGTGATGGGCACCGAGCACCCCAACACCATCCAGGAATACATGCACCTGGCCCTGTACTGCTTCG
  CCAGCAGCCAGCTGTCCACCGCCCTGAGCCTGCTGTACCGCGCCCGCTACCTCATGCTGCTGGTGTTCGG
  GGAAGACCACCCCGAGATGGCGCTGCTGGACAACAACATCGGGCTGGTGCTGCACGGGGTGATGGAGTAC
  GACCTGTCGCTGCGCTTCCTGGAGAACGCGCTGGCCGTCAGCACCAAGTACCACGGGCCCAAGGCCCTCA
  AGGTGGCCCTCAGCCACCACCTTGTCGCCCGAGTCTACGAGAGCAAAGCTGAGTTCCGGTCGGCCCTGCA
  GCACGAGAAGGAGGGTTACACCATCTACAAGACGCAGCTGGGCGAGGACCATGAGAAGACCAAGGAAAGC
  TCCGAGTACCTCAAGTGCCTGACCCAGCAGGCCGTGGCCCTGCAGCGCACCATGAACGAGATCTACCGCA
  ACGGCTCCAGCGCCAACATCCCGCCCCTCAAGTTCACGGCCCCCAGCATGGCCAGCGTCTTGGAGCAGCT
  GAACGTCATTAACGGCATCCTCTTCATTCCTCTCAGCCAAAAAGACCTGGAGAATCTGAAAGCCGAGGTG
  GCGCGGCGGCACCAGCTCCAGGAGGCCAGCAGAAACAGGGATAGAGCCGAGGAGCCCATGGCTACCGAGC
  CCGCGCCAGCGGGGGCCCCAGGAGACCTGGGCTCCCAGCCCCCGGCTGCCAAGGACCCTTCTCCGAGCGT
  GCAGGGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG220737 representing NM_015229
  Red=Cloning site Green=Tags(s)
  
  MLLNGDCPESLKKEAAAAEPPRENGLDEAGPGDETTGQEVIVIQDTGFSVKILAPGIEPFSLQVSPQEMV
  QEIHQVLMDREDTCHRTCFSLHLDGNVLDHFSELRSVEGLQEGSVLRVVEEPYTVREARIHVRHVRDLLK
  SLDPSDAFNGVDCNSLSFLSVFTDGDLGDSGKRKKGLEMDPIDCTPPEYILPGSRERPLCPLQPQNRDWK
  PLQCLKVLTMSGWNPPPGNRKMHGDLMYLFVITAEDRQVSITASTRGFYLNQSTAYHFNPKPASPRFLSH
  SLVELLNQISPTFKKNFAVLQKKRVQRHPFERIATPFQVYSWTAPQAEHAMDCVRAEDAYTSRLGYEEHI
  PGQTRDWNEELQTTRELPRKNLPERLLRERAIFKVHSDFTAAATRGAMAVIDGNVMAINPSEETKMQMFI
  WNNIFFSLGFDVRDHYKDFGGDVAAYVAPTNDLNGVRTYNAVDVEGLYTLGTVVVDYRGYRVTAQSIIPG
  ILERDQEQSVIYGSIDFGKTVVSHPRYLELLERTSRPLKILRHQVLNDRDEEVELCSSVECKGIIGNDGR
  HYILDLLRTFPPDLNFLPVPGEELPEECARAGFPRAHRHKLCCLRQELVDAFVEHRYLLFMKLAALQLMQ
  QNASQLETPSSLENGGPSSLESKSEDPPGQEAGSEEEGSSASGLAKVKELAETIAADDGTDPRSREVIRN
  ACKAVGSISSTAFDIRFNPDIFSPGVRFPESCQDEVRDQKQLLKDAAAFLLSCQIPGLVKDCMEHAVLPV
  DGATLAEVMRQRGINMRYLGKVLELVLRSPARHQLDHVFKIGIGELITRSAKHIFKTYLQGVELSGLSAA
  ISHFLNCFLSSYPNPVAHLPADELVSKKRNKRRKNRPPGAADNTAWAVMTPQELWKNICQEAKNYFDFDL
  ECETVDQAVETYGLQKITLLREISLKTGIQVLLKEYSFDSRHKPAFTEEDVLNIFPVVKHVNPKASDAFH
  FFQSGQAKVQQGFLKEGCELINEALNLFNNVYGAMHVETCACLRLLARLHYIMGDYAEALSNQQKAVLMS
  ERVMGTEHPNTIQEYMHLALYCFASSQLSTALSLLYRARYLMLLVFGEDHPEMALLDNNIGLVLHGVMEY
  DLSLRFLENALAVSTKYHGPKALKVALSHHLVARVYESKAEFRSALQHEKEGYTIYKTQLGEDHEKTKES
  SEYLKCLTQQAVALQRTMNEIYRNGSSANIPPLKFTAPSMASVLEQLNVINGILFIPLSQKDLENLKAEV
  ARRHQLQEASRNRDRAEEPMATEPAPAGAPGDLGSQPPAAKDPSPSVQG
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015229
• ORF Size: 3927 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015229.3, NP_056044.3
• RefSeq Size: 5252 bp
• RefSeq ORF: 4044 bp
• Locus ID: 23277
• UniProt ID: O75153
• Cytogenetics: 17p13.3
• Protein Families: Druggable Genome
• Gene Summary: mRNA-binding protein involved in proper cytoplasmic distribution of mitochondria. Specifically binds mRNAs of nuclear-encoded mitochondrial proteins in the cytoplasm and regulates transport or translation of these transcripts close to mitochondria, playing a role in mitochondrial biogenesis.[UniProtKB/Swiss-Prot Function]