OriGene - PGK1 (NM_000291) Human ORF cDNA Clone

Western Blot Validated

TrueORF Data for RG211172

Vector:	pCMV6-AC-GFP Change vector?	Tag: C-terminal TurboGFP	Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Sequence Data:	ORF Nucleotide Sequence CHROMATOGRAMS Protein Sequence	ORF Size: 1254 bp Predicted Protein MW: 44.4 kDa
Restriction Sites:	SgfI-MluI Cloning Scheme for this gene Plasmid Map
OTI Annotation:	This clone was engineered to express the complete ORF with an expression tag.
OTI Disclaimer:	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components:	The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Protein Families:	Druggable Genome
Protein Pathways:	Glycolysis / Gluconeogenesis Metabolic pathways

* The lysates used for this WB picture contain the overexpressed empty vector or the Myc-DDK tagged ORF clone.

Reference Data

RefSeq: NM_000291.2, NP_000282	RefSeq Size: 2338	RefSeq ORF: 1254
Synonyms : MIG10; PGKA
LocusID: 5230	Cytogenetic: Xq13.3	Domains: PGK
Summary: The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. This gene lies on the X-chromosome, while a related pseudogene also has been found on the X-chromosome and another on chromosome 19. [provided by RefSeq, Oct 2008].

ORF Clones Citations:

Selenoprotein K is required for palmitoylation of CD36 in macrophages: implications in foam cell formation and atherogenesis, Svenja Meiler, Yvonne Baumer, Zhi Huang, Fukun W. Hoffmann, Gregory J. Fredericks, Aaron H. Rose, Robert L. Norton, Peter R. Hoffmann, and William A. Boisvert, J. Leukoc. Biol., May 2013; 93: 771 - 780. [CD36]

Trafficking of the Transcription Factor Nrf2 to Promyelocytic Leukemia-Nuclear Bodies: IMPLICATIONS FOR DEGRADATION OF NRF2 IN THE NUCLEUS, Melanie Theodore Malloy, Deneshia J. McIntosh, Treniqka S. Walters, Andrea Flores, J. Shawn Goodwin, and Ifeanyi J. Arinze, J. Biol. Chem., May 2013; 288: 14569 - 14583. [RNF4]

Cofactor Balance by Nicotinamide Nucleotide Transhydrogenase (NNT) Coordinates Reductive Carboxylation and Glucose Catabolism in the Tricarboxylic Acid (TCA) Cycle, Paulo A. Gameiro, Laura A. Laviolette, Joanne K. Kelleher, Othon Iliopoulos, and Gregory Stephanopoulos, J. Biol. Chem., May 2013; 288: 12967 - 12977. [NNT]

Inverted Formin 2 Regulates Actin Dynamics by Antagonizing Rho/Diaphanous-related Formin Signaling, Hua Sun, Johannes Schlondorff, Henry N. Higgs, and Martin R. Pollak, J. Am. Soc. Nephrol., May 2013; 24: 917 - 929. [INF2]

DEK Depletion Negatively Regulates Rho/ROCK/MLC Pathway in Non–Small Cell Lung Cancer, Junying Wang, Limei Sun, Mingyue Yang, Wenting Luo, Ying Gao, Zihui Liu, Xueshan Qiu, and Enhua Wang, Journal of Histochemistry & Cytochemistry, May 2013; 10.1369/0022155413488120. [DEK]

A model of lysosomal pH regulation, Yoichi Ishida, Smita Nayak, Joseph A. Mindell, and Michael Grabe, J. Gen. Physiol., May 2013; 141: 705 - 720. [CLCN7]

A model of lysosomal pH regulation, Yoichi Ishida, Smita Nayak, Joseph A. Mindell, and Michael Grabe, J. Gen. Physiol., May 2013; 141: 705 - 720. [OSTM1]

The Serum- and Glucocorticoid-inducible Kinases SGK1 and SGK3 Regulate hERG Channel Expression via Ubiquitin Ligase Nedd4-2 and GTPase Rab11, Shawn M. Lamothe and Shetuan Zhang, J. Biol. Chem., May 2013; 288: 15075 - 15084. [SGK1]

Regulation of the Bone-restricted IFITM-like (Bril) Gene Transcription by Sp and Gli Family Members and CpG Methylation, Bahar Kasaai, Marie-Hélène Gaumond, and Pierre Moffatt, J. Biol. Chem., May 2013; 288: 13278 - 13294. [SP3]

More TrueClone Citations >>

PGK1 Mouse Clones

SKU	Description	Amount	Price	Shipping
MC207348	Mus musculus phosphoglycerate kinase 1 (Pgk1) as transfection-ready DNA	10 ug	$380	4 weeks
MC218218	Mus musculus phosphoglycerate kinase 1 (cDNA clone MGC:102158 IMAGE:6828087) as transfection-ready DNA	10 ug	$650	3 weeks
MR206593	Myc-DDK-tagged ORF clone of Mus musculus phosphoglycerate kinase 1 (Pgk1) as transfection-ready DNA	10 ug	$380	Next day
MR206611	Myc-DDK-tagged ORF clone of Mus musculus phosphoglycerate kinase 1 (cDNA clone MGC:102158 IMAGE:6828087) as transfection-ready DNA	10 ug	$650	3 weeks
MG206593	GFP-tagged ORF clone of Mus musculus phosphoglycerate kinase 1 (cDNA clone MGC:118097 IMAGE:5060103) in a GFP expression vector	10 ug	$420	3 weeks
MG206611	GFP-tagged ORF clone of Mus musculus phosphoglycerate kinase 1 (cDNA clone MGC:102158 IMAGE:6828087) in a GFP expression vector	10 ug	$720	3 weeks

PGK1 Rat Clones

SKU	Description	Amount	Price	Shipping
RN210973	NativeORF clone of Rattus norvegicus phosphoglycerate kinase 1 (Pgk1) as transfection-ready DNA	10 ug	$380	Next day
RR210973	Myc-DDK-tagged ORF clone of Rattus norvegicus phosphoglycerate kinase 1 (Pgk1) as transfection-ready DNA	10 ug	$380	3 weeks

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PGK1 (NM_000291) Human cDNA ORF Clone

Western Blot Validated

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