OriGene - NEFL (NM_006158) Human ORF cDNA Clone

Western Blot Validated

TrueORF Data for RG205920

Vector:	pCMV6-AC-GFP Change vector?	Tag: C-terminal TurboGFP	Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Sequence Data:	ORF Nucleotide Sequence CHROMATOGRAMS Protein Sequence	ORF Size: 1632 bp Predicted Protein MW: 61.3 kDa
Restriction Sites:	SgfI-MluI Cloning Scheme for this gene Plasmid Map
OTI Annotation:	This clone was engineered to express the complete ORF with an expression tag.
OTI Disclaimer:	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components:	The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Protein Families:	ES Cell Differentiation/IPS Druggable Genome
Protein Pathways:	Amyotrophic lateral sclerosis (ALS)

* The lysates used for this WB picture contain the overexpressed empty vector or the Myc-DDK tagged ORF clone.

Reference Data

RefSeq: NM_006158.2, NP_006149	RefSeq Size: 3584	RefSeq ORF: 1632
Synonyms : CMT1F; CMT2E; NF-L; NF68; NFL
LocusID: 4747	Cytogenetic: 8p21	Domains: filament, filament_head
Summary: Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq, Oct 2008].

ORF Clones Citations:

Solute Carrier 11A1 Is Expressed by Innate Lymphocytes and Augments Their Activation, Jodi F. Hedges, Emily Kimmel, Deann T. Snyder, Maria Jerome, and Mark A. Jutila, J. Immunol., Apr 2013; 190: 4263 - 4273. [SLC11A1]

A common polymorphism in the LDL receptor gene has multiple effects on LDL receptor function, Feng Gao, Hansel E. Ihn, Marisa W. Medina, and Ronald M. Krauss, Hum. Mol. Genet., Apr 2013; 22: 1424 - 1431. [LDLR]

RNA elements directing in vivo assembly of the 7SK/MePCE/Larp7 transcriptional regulatory snRNP, Lisa Muniz, Sylvain Egloff, and Tamás Kiss, Nucleic Acids Res., Apr 2013; 41: 4686 - 4698. [MEPCE ]

Structural basis for angiopoietin-1–mediated signaling initiation, Xuehong Yu, Tom C. M. Seegar, Annamarie C. Dalton, Dorothea Tzvetkova-Robev, Yehuda Goldgur, Kanagalaghatta R. Rajashankar, Dimitar B. Nikolov, and William A. Barton, PNAS, Apr 2013; 110: 7205 - 7210. [ANGPT1]

Inverted Formin 2 Regulates Actin Dynamics by Antagonizing Rho/Diaphanous-related Formin Signaling, Hua Sun, Johannes Schlondorff, Henry N. Higgs, and Martin R. Pollak, J. Am. Soc. Nephrol., Apr 2013; 10.1681/ASN.2012080834. [INF2]

MASL1 induces erythroid differentiation in human erythropoietin-dependent CD34+ cells through the Raf/MEK/ERK pathway, Chutima Kumkhaek, Wulin Aerbajinai, Wenli Liu, Jianqiong Zhu, Naoya Uchida, Roger Kurlander, Matthew M. Hsieh, John F. Tisdale, and Griffin P. Rodgers, Blood, Apr 2013; 121: 3216 - 3227. [MFHAS1 ]

Proximal tubule-dominant transfer of AT1a receptors induces blood pressure responses to intracellular angiotensin II in AT1a receptor-deficient mice, Xiao C. Li and Jia L. Zhuo, Am J Physiol Regulatory Integrative Comp Physiol, Apr 2013; 304: R588 - R598. [AGTR1A ]

DCNL1 Functions as a Substrate Sensor and Activator of Cullin 2-RING Ligase, Pardeep Heir, Roxana I. Sufan, Samantha N. Greer, Betty P. Poon, Jeffrey E. Lee, and Michael Ohh, Mol. Cell. Biol., Apr 2013; 33: 1621 - 1631. [DCNL1]

More TrueClone Citations >>

NEFL Mouse Clones

SKU	Description	Amount	Price	Shipping
MC218208	Mus musculus neurofilament, light polypeptide (Nefl) as transfection-ready DNA	10 ug	$650	5 weeks
MR224212	Myc-DDK-tagged ORF clone of Mus musculus neurofilament, light polypeptide (Nefl) as transfection-ready DNA	10 ug	$650	5 weeks
MG224212	GFP-tagged ORF clone of Mus musculus neurofilament light polypeptide (Nefl) as transfection-ready DNA	10 ug	$720	3 weeks

NEFL Rat Clones

SKU	Description	Amount	Price	Shipping
RN207028	NativeORF clone of Rattus norvegicus neurofilament, light polypeptide (Nefl) as transfection-ready DNA	10 ug	$652	5 weeks
RR207028	Myc-DDK-tagged ORF clone of Rattus norvegicus neurofilament, light polypeptide (Nefl) as transfection-ready DNA	10 ug	$652	5 weeks

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NEFL (NM_006158) Human cDNA ORF Clone

Western Blot Validated

ORF Clones Citations:

NEFL Mouse Clones

NEFL Rat Clones

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