Properdin (CFP) (NM_002621) Human Tagged ORF Clone
CAT#: RG204890
- TrueORF®
CFP (tGFP-tagged) - Human complement factor properdin (CFP), transcript variant 1
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_002621" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Properdin |
Synonyms | BFD; PFC; PFD; PROPERDIN |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG204890 representing NM_002621
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCACAGAGGGAGCGCAGGCCCCTCGATTGTTGCTGCCGCCGCTGCTCCTGCTGCTCACCCTGCCAG CCACAGGCTCAGACCCCGTGCTCTGCTTCACCCAGTATGAAGAATCCTCCGGCAAGTGCAAGGGCCTCCT GGGGGGTGGTGTCAGCGTGGAAGACTGCTGTCTCAACACTGCCTTTGCCTACCAGAAACGTAGTGGTGGG CTCTGTCAGCCTTGCAGGTCCCCACGATGGTCCCTTTGGTCCACATGGGCCCCCTGTTCGGTGACGTGCT CTGAGGGCTCCCAGCTGCGGTACCGGCGCTGTGTGGGCTGGAATGGGCAGTGCTCTGGAAAGGTGGCACC TGGGACCCTGGAGTGGCAGCTCCAGGCCTGTGAGGACCAGCAGTGCTGTCCTGAGATGGGCGGCTGGTCT GGCTGGGGGCCCTGGGAGCCTTGCTCTGTCACCTGCTCCAAAGGGACCCGGACCCGCAGGCGAGCCTGTA ATCACCCTGCTCCCAAGTGTGGGGGCCACTGCCCAGGACAGGCACAGGAATCAGAGGCCTGTGACACCCA GCAGGTCTGCCCCACACACGGGGCCTGGGCCACCTGGGGCCCCTGGACCCCCTGCTCAGCCTCCTGCCAC GGTGGACCCCACGAACCTAAGGAGACACGAAGCCGCAAGTGTTCTGCACCTGAGCCCTCCCAGAAACCTC CTGGGAAGCCCTGCCCGGGGCTAGCCTACGAGCAGCGGAGGTGCACCGGCCTGCCACCCTGCCCAGTGGC TGGGGGCTGGGGGCCTTGGGGCCCTGTGAGCCCCTGCCCTGTGACCTGTGGCCTGGGCCAGACCATGGAA CAACGGACGTGCAATCACCCTGTGCCCCAGCATGGGGGCCCCTTCTGTGCTGGCGATGCCACCCGGACCC ACATCTGCAACACAGCTGTGCCCTGCCCTGTGGATGGGGAGTGGGACTCGTGGGGGGAGTGGAGCCCCTG TATCCGACGGAACATGAAGTCCATCAGCTGTCAAGAAATCCCGGGCCAGCAGTCACGCGGGAGGACCTGC AGGGGCCGCAAGTTTGACGGACATCGATGTGCCGGGCAACAGCAGGATATCCGGCACTGCTACAGCATCC AGCACTGCCCCTTGAAAGGATCATGGTCAGAGTGGAGTACCTGGGGGCTGTGCATGCCCCCCTGTGGACC TAATCCTACCCGTGCCCGCCAGCGCCTCTGCACACCCTTGCTCCCCAAGTACCCGCCCACCGTTTCCATG GTCGAAGGTCAGGGCGAGAAGAACGTGACCTTCTGGGGGAGACCGCTGCCACGGTGTGAGGAGCTACAAG GGCAGAAGCTGGTGGTGGAGGAGAAACGACCATGTCTACACGTGCCTGCTTGCAAAGACCCTGAGGAAGA GGAACTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG204890 representing NM_002621
Red=Cloning site Green=Tags(s) MITEGAQAPRLLLPPLLLLLTLPATGSDPVLCFTQYEESSGKCKGLLGGGVSVEDCCLNTAFAYQKRSGG LCQPCRSPRWSLWSTWAPCSVTCSEGSQLRYRRCVGWNGQCSGKVAPGTLEWQLQACEDQQCCPEMGGWS GWGPWEPCSVTCSKGTRTRRRACNHPAPKCGGHCPGQAQESEACDTQQVCPTHGAWATWGPWTPCSASCH GGPHEPKETRSRKCSAPEPSQKPPGKPCPGLAYEQRRCTGLPPCPVAGGWGPWGPVSPCPVTCGLGQTME QRTCNHPVPQHGGPFCAGDATRTHICNTAVPCPVDGEWDSWGEWSPCIRRNMKSISCQEIPGQQSRGRTC RGRKFDGHRCAGQQQDIRHCYSIQHCPLKGSWSEWSTWGLCMPPCGPNPTRARQRLCTPLLPKYPPTVSM VEGQGEKNVTFWGRPLPRCEELQGQKLVVEEKRPCLHVPACKDPEEEEL TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_002621 |
ORF Size | 1407 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_002621.1, NP_002612.1 |
RefSeq Size | 1701 bp |
RefSeq ORF | 1410 bp |
Locus ID | 5199 |
UniProt ID | P27918 |
Cytogenetics | Xp11.23 |
Domains | tsp_1 |
Protein Families | Secreted Protein |
Gene Summary | This gene encodes a plasma glycoprotein that positively regulates the alternative complement pathway of the innate immune system. This protein binds to many microbial surfaces and apoptotic cells and stabilizes the C3- and C5-convertase enzyme complexes in a feedback loop that ultimately leads to formation of the membrane attack complex and lysis of the target cell. Mutations in this gene result in two forms of properdin deficiency, which results in high susceptibility to meningococcal infections. Multiple alternatively spliced variants, encoding the same protein, have been identified.[provided by RefSeq, Feb 2009] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC204890 | CFP (Myc-DDK-tagged)-Human complement factor properdin (CFP), transcript variant 1 |
USD 457.00 |
|
RC204890L3 | Lenti ORF clone of Human complement factor properdin (CFP), transcript variant 1, Myc-DDK-tagged |
USD 757.00 |
|
RC204890L4 | Lenti ORF clone of Human complement factor properdin (CFP), transcript variant 1, mGFP tagged |
USD 757.00 |
|
SC319542 | CFP (untagged)-Human complement factor properdin (CFP), transcript variant 1 |
USD 457.00 |
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