PARL (NM_018622) Human Tagged ORF Clone
CAT#: RG204096
- TrueORF®
PARL (tGFP-tagged) - Human presenilin associated, rhomboid-like (PARL), nuclear gene encoding mitochondrial protein, transcript variant 1
ORF Plasmid: DDK
Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro
AAV Particle: DDK
"NM_018622" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | PARL |
Synonyms | PRO2207; PSARL; PSARL1; PSENIP2; RHBDS1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG204096 representing NM_018622
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTGGCGAGGCTGGGCGCAGAGAGGCTGGGGCTGCGGCCAGGCGTGGGGTGCGTCGGTGGGCGGCC GCAGCTGCGAGGAGCTCACTGCGGTCCTAACCCCGCCGCAGCTCCTCGGACGCAGGTTTAACTTCTTTAT TCAACAAAAATGCGGATTCAGAAAAGCACCCAGGAAGGTTGAACCTCGAAGATCAGACCCAGGGACAAGT GGTGAAGCATACAAGAGAAGTGCTTTGATTCCTCCTGTGGAAGAAACAGTCTTTTATCCTTCTCCCTATC CTATAAGGAGTCTCATAAAACCTTTATTTTTTACTGTTGGGTTTACAGGCTGTGCATTTGGATCAGCTGC TATTTGGCAATATGAATCACTGAAATCCAGGGTCCAGAGTTATTTTGATGGTATAAAAGCTGATTGGTTG GATAGCATAAGACCACAAAAAGAAGGAGACTTCAGAAAGGAGATTAACAAGTGGTGGAATAACCTAAGTG ATGGCCAGCGGACTGTGACAGGTATTATAGCTGCAAATGTCCTTGTATTCTGTTTATGGAGAGTACCTTC TCTGCAGCGGACAATGATCAGATATTTCACATCGAATCCAGCCTCAAAGGTCCTTTGTTCTCCAATGTTG CTGTCAACATTCAGTCATTTCTCCTTATTTCACATGGCAGCAAATATGTATGTTTTGTGGAGCTTCTCTT CCAGCATAGTGAACATTCTGGGTCAAGAGCAGTTCATGGCAGTGTACCTATCTGCAGGTGTTATTTCCAA TTTTGTCAGTTACGTGGGTAAAGTTGCCACAGGAAGATATGGACCATCACTTGGTGCATCTGGTGCCATC ATGACAGTCCTCGCAGCTGTCTGCACTAAGATCCCAGAAGGGAGGCTTGCCATTATTTTCCTTCCGATGT TCACGTTCACAGCAGGGAATGCCCTGAAAGCCATTATCGCCATGGATACAGCAGGAATGATCCTGGGATG GAAATTTTTTGATCATGCGGCACATCTTGGGGGAGCTCTTTTTGGAATATGGTATGTTACTTACGGTCAT GAACTGATTTGGAAGAACAGGGAGCCGCTAGTGAAAATCTGGCATGAAATAAGGACTAATGGCCCCAAAA AAGGAGGTGGCTCTAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG204096 representing NM_018622
Red=Cloning site Green=Tags(s) MAWRGWAQRGWGCGQAWGASVGGRSCEELTAVLTPPQLLGRRFNFFIQQKCGFRKAPRKVEPRRSDPGTS GEAYKRSALIPPVEETVFYPSPYPIRSLIKPLFFTVGFTGCAFGSAAIWQYESLKSRVQSYFDGIKADWL DSIRPQKEGDFRKEINKWWNNLSDGQRTVTGIIAANVLVFCLWRVPSLQRTMIRYFTSNPASKVLCSPML LSTFSHFSLFHMAANMYVLWSFSSSIVNILGQEQFMAVYLSAGVISNFVSYVGKVATGRYGPSLGASGAI MTVLAAVCTKIPEGRLAIIFLPMFTFTAGNALKAIIAMDTAGMILGWKFFDHAAHLGGALFGIWYVTYGH ELIWKNREPLVKIWHEIRTNGPKKGGGSK TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_018622 |
ORF Size | 1137 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_018622.7 |
RefSeq Size | 1423 bp |
RefSeq ORF | 1140 bp |
Locus ID | 55486 |
UniProt ID | Q9H300 |
Cytogenetics | 3q27.1 |
Domains | Rhomboid |
Protein Families | Protease, Transmembrane |
Gene Summary | This gene encodes a member of the rhomboid family of intramembrane serine proteases that is localized to the inner mitochondrial membrane. The encoded protein regulates mitochondrial remodeling and apoptosis through regulated substrate proteolysis. Proteolytic processing of the encoded protein results in the release of a small peptide, P-beta, which may transit to the nucleus. Mutations in this gene may be associated with Parkinson's disease. [provided by RefSeq, May 2016] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC204096 | PARL (Myc-DDK-tagged)-Human presenilin associated, rhomboid-like (PARL), nuclear gene encoding mitochondrial protein, transcript variant 1 |
USD 457.00 |
|
RC204096L1 | Lenti ORF clone of Human presenilin associated, rhomboid-like (PARL), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged |
USD 757.00 |
|
RC204096L2 | Lenti ORF clone of Human presenilin associated, rhomboid-like (PARL), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged |
USD 757.00 |
|
RC204096L3 | Lenti ORF clone of Human presenilin associated, rhomboid-like (PARL), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged |
USD 757.00 |
|
RC204096L4 | Lenti ORF clone of Human presenilin associated, rhomboid-like (PARL), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged |
USD 757.00 |
|
SC108782 | PARL (untagged)-Human presenilin associated, rhomboid-like (PARL), nuclear gene encoding mitochondrial protein, transcript variant 1 |
USD 457.00 |
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