OriGene - GAPDH (NM_002046) Human ORF cDNA Clone

Western Blot Validated

TrueORF Data for RG202309

Vector:	pCMV6-AC-GFP Change vector?	Tag: C-terminal TurboGFP	Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Sequence Data:	ORF Nucleotide Sequence CHROMATOGRAMS Protein Sequence	ORF Size: 1008 bp Predicted Protein MW: kDa
Restriction Sites:	SgfI-MluI Cloning Scheme for this gene Plasmid Map
OTI Annotation:	This clone was engineered to express the complete ORF with an expression tag.
OTI Disclaimer:	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components:	The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Protein Families:	ES Cell Differentiation/IPS
Protein Pathways:	Glycolysis / Gluconeogenesis Metabolic pathways Alzheimer's disease

* The lysates used for this WB picture contain the overexpressed empty vector or the Myc-DDK tagged ORF clone.

Reference Data

RefSeq: NM_002046.2, NP_002037	RefSeq Size: 1283	RefSeq ORF: 1008
Synonyms : G3PD; GAPD
LocusID: 2597	Cytogenetic: 12p13	Domains: gpdh_C
Summary: The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012].

ORF Clones Citations:

Intracellular Trafficking of the KV1.3 Potassium Channel Is Regulated by the Prodomain of a Matrix Metalloprotease, Hai M. Nguyen, Charles A. Galea, Galina Schmunk, Brian J. Smith, Robert A. Edwards, Raymond S. Norton, and K. George Chandy, J. Biol. Chem., Mar 2013; 288: 6451 - 6464. [KV1.3]

Renal Hypodysplasia Associates with a Wnt4 Variant that Causes Aberrant Canonical Wnt Signaling, Asaf Vivante, Michal Mark-Danieli, Miriam Davidovits, Orit Harari-Steinberg, Dorit Omer, Yehudit Gnatek, Roxana Cleper, Daniel Landau, Yael Kovalski, Irit Weissman, Israel Eisenstein, Michalle Soudack, Haike Reznik Wolf, Naomi Issler, Danny Lotan, Yair Anikster, and Benjamin Dekel, J. Am. Soc. Nephrol., Mar 2013; 24: 550 - 558. [WNT4]

Smad4-Irf6 genetic interaction and TGFß-mediated IRF6 signaling cascade are crucial for palatal fusion in mice, Jun-ichi Iwata, Akiko Suzuki, Richard C. Pelikan, Thach-Vu Ho, Pedro A. Sanchez-Lara, Mark Urata, Michael J. Dixon, and Yang Chai, Development, Mar 2013; 140: 1220 - 1230. [p21]

Mutation of the iron-sulfur cluster assembly gene IBA57 causes severe myopathy and encephalopathy, Nikhita Ajit Bolar, Arnaud Vincent Vanlander, Claudia Wilbrecht, Nathalie Van der Aa, Joél Smet, Boel De Paepe, Geert Vandeweyer, Frank Kooy, François Eyskens, Elien De Latter, Gwenda Delanghe, Paul Govaert, Jules Gerard Leroy, Bart Loeys, Roland Lill, Lut Van Laer, and Rudy Van Coster, Hum. Mol. Genet., Mar 2013; 10.1093/hmg/ddt107. [IBA57]

Mutations in Critical Domains Confer the Human mTOR Gene Strong Tumorigenicity, Avaniyapuram Kannan Murugan, Ali Alzahrani, and Mingzhao Xing, J. Biol. Chem., Mar 2013; 288: 6511 - 6521. [mTOR]

Specificity Profiling of Dual Specificity Phosphatase Vaccinia VH1-related (VHR) Reveals Two Distinct Substrate Binding Modes, Rinrada Luechapanichkul, Xianwen Chen, Hashem A. Taha, Shubham Vyas, Xiaoyan Guan, Michael A. Freitas, Christopher M. Hadad, and Dehua Pei, J. Biol. Chem., Mar 2013; 288: 6498 - 6510. [ROBO1]

Dissecting the Role of COPI Complexes in Influenza Virus Infection, Eileen Sun, Jiang He, and Xiaowei Zhuang, J. Virol., Mar 2013; 87: 2673 - 2685. [COPE]

ATF4 Protects Against Neuronal Death in Cellular Parkinson's Disease Models by Maintaining Levels of Parkin, Xiaotian Sun, Jin Liu, John F. Crary, Cristina Malagelada, David Sulzer, Lloyd A. Greene, and Oren A. Levy, J. Neurosci., Feb 2013; 33: 2398 - 2407. [Myc-tagged rat parkin]

Functional analysis of sucrase–isomaltase mutations from chronic lymphocytic leukemia patients, David Rodríguez, Andrew J. Ramsay, Víctor Quesada, Cecilia Garabaya, Elías Campo, José M. P. Freije, and Carlos López-Otín, Hum. Mol. Genet., Feb 2013; 10.1093/hmg/ddt078. [SI]

Mutation of the ATP-gated P2X2 receptor leads to progressive hearing loss and increased susceptibility to noise, Denise Yan, Yan Zhu, Tom Walsh, Dinghua Xie, Huijun Yuan, Asli Sirmaci, Taro Fujikawa, Ann Chi Yan Wong, Tze L. Loh, Lilin Du, M’hamed Grati, Srdjan M. Vlajkovic, Susan Blanton, Allen F. Ryan, Zheng-Yi Chen, Peter R. Thorne, Bechara Kachar, Mustafa Tekin, Hong-Bo Zhao, Gary D. Housley, Mary-Claire King, and Xue Z. Liu, PNAS, Feb 2013; 110: 2228 - 2233. [P2RX2 ]

More TrueClone Citations >>

GAPDH Mouse Clones

SKU	Description	Amount	Price	Shipping
MC218171	Mus musculus glyceraldehyde-3-phosphate dehydrogenase (cDNA clone MGC:103188 IMAGE:4159824) as transfection-ready DNA	10 ug	$650	3 weeks
MC207054	Mus musculus glyceraldehyde-3-phosphate dehydrogenase (cDNA clone MGC:107018 IMAGE:1067416) as transfection-ready DNA	10 ug	$300	3 weeks
MC207283	Mus musculus glyceraldehyde-3-phosphate dehydrogenase (Gapdh) as transfection-ready DNA	10 ug	$380	Next day
MR204927	Myc-DDK-tagged ORF clone of Mus musculus glyceraldehyde-3-phosphate dehydrogenase (cDNA clone MGC:103188 IMAGE:4159824) as transfection-ready DNA	10 ug	$650	3 weeks
MR204934	Myc-DDK-tagged ORF clone of Mus musculus glyceraldehyde-3-phosphate dehydrogenase (Gapdh) as transfection-ready DNA	10 ug	$380	Next day
MR200822	Myc-DDK-tagged ORF clone of Mus musculus glyceraldehyde-3-phosphate dehydrogenase (cDNA clone MGC:107018 IMAGE:1067416) as transfection-ready DNA	10 ug	$300	3 weeks
MG200822	GFP-tagged ORF clone of Mus musculus glyceraldehyde-3-phosphate dehydrogenase (cDNA clone MGC:107018 IMAGE:1067416) in a GFP expression vector	10 ug	$330	Next day
MG204927	GFP-tagged ORF clone of Mus musculus glyceraldehyde-3-phosphate dehydrogenase (cDNA clone MGC:103188 IMAGE:4159824) in a GFP expression vector	10 ug	$720	3 weeks
MG204934	GFP-tagged ORF clone of Mus musculus glyceraldehyde-3-phosphate dehydrogenase (Gapdh) in a GFP expression vector	10 ug	$420	Next day

GAPDH Rat Clones

SKU	Description	Amount	Price	Shipping
RN206447	NativeORF clone of Rattus norvegicus glyceraldehyde-3-phosphate dehydrogenase (Gapdh) as transfection-ready DNA	10 ug	$380	Next day
RR206447	Myc-DDK-tagged ORF clone of Rattus norvegicus glyceraldehyde-3-phosphate dehydrogenase (Gapdh) as transfection-ready DNA	10 ug	$380	3 weeks

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GAPDH (NM_002046) Human cDNA ORF Clone

Western Blot Validated

ORF Clones Citations:

GAPDH Mouse Clones

GAPDH Rat Clones

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