OriGene - CDKN1A (NM_000389) Human ORF cDNA Clone

CDKN1A (NM_000389) Human cDNA ORF Clone

TrueORF Data for RG201817

Vector:	pCMV6-AC-GFP Change vector?	Tag: C-terminal TurboGFP	Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Sequence Data:	ORF Nucleotide Sequence CHROMATOGRAMS Protein Sequence	ORF Size: 495 bp Predicted Protein MW: 17.9 kDa
Restriction Sites:	SgfI-MluI Cloning Scheme for this gene Plasmid Map
OTI Annotation:	This clone was engineered to express the complete ORF with an expression tag.
OTI Disclaimer:	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components:	The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Protein Families:	Druggable Genome
Protein Pathways:	ErbB signaling pathway Cell cycle p53 signaling pathway Pathways in cancer Glioma Prostate cancer More Pathways >>

* The lysates used for this WB picture contain the overexpressed empty vector or the Myc-DDK tagged ORF clone.

Reference Data

RefSeq: NM_000389.2, NP_000380	RefSeq Size: 2140	RefSeq ORF: 495
Synonyms : CAP20; CDKN1; CIP1; MDA-6; P21; p21CIP1; SDI1; WAF1
LocusID: 1026	Cytogenetic: 6p21.2	Domains: CDI
Summary: This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Multiple alternatively spliced variants have been found for this gene. [provided by RefSeq, Nov 2010].

SKU	Description	Amount	Price	Shipping
MC200171	Mus musculus cyclin-dependent kinase inhibitor 1A (P21) (Cdkn1a), transcript variant 1 as transfection-ready DNA	10 ug	$165	Next day
MR201211	Myc-DDK-tagged ORF clone of Mus musculus cyclin-dependent kinase inhibitor 1A (P21) (Cdkn1a), transcript variant 2 as transfection-ready DNA	10 ug	$380	Next day
MR227529	Myc-DDK-tagged ORF clone of Mus musculus cyclin-dependent kinase inhibitor 1A (P21) (Cdkn1a), transcript variant 1 as transfection-ready DNA	10 ug	$380	Next day
MG227529	GFP-tagged ORF clone of Mus musculus cyclin-dependent kinase inhibitor 1A (P21) (Cdkn1a) transcript variant 1 as transfection-ready DNA	10 ug	$420	3 weeks
MG201211	GFP-tagged ORF clone of Mus musculus cyclin-dependent kinase inhibitor 1A (P21) (Cdkn1a) in a GFP expression vector	10 ug	$420	Next day

SKU	Description	Amount	Price	Shipping
RN209648	NativeORF clone of Rattus norvegicus cyclin-dependent kinase inhibitor 1A (p21, Cip1) (Cdkn1a) as transfection-ready DNA	10 ug	$380	2 weeks
RR209648	Myc-DDK-tagged ORF clone of Rattus norvegicus cyclin-dependent kinase inhibitor 1A (p21, Cip1) (Cdkn1a) as transfection-ready DNA	10 ug	$380	2 weeks

Don't see the species of your interest?
Use Blue Heron gene synthesis and make clone in any of OriGene's expression vectors.

CDKN1A (NM_000389) Human cDNA ORF Clone