ELL3 (NM_025165) Human Tagged ORF Clone

CAT#: RG200765

ELL3 (tGFP-tagged) - Human elongation factor RNA polymerase II-like 3 (ELL3)

Product Images

Circular map for RG200765

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ELL3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG200765 representing NM_025165
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGAGCTCCAGGAGCCTCTGAGAGGACAGCTCCGGCTCTGCTTCACGCAAGCTGCCCGGACTAGCC
  TCTTACTGCTCAGGCTCAACGACGCTGCCCTGCGGGCGCTGCAAGAGTGTCAGCGGCAACAGGTACGGCC
  GGTGATTGCTTTCCAAGGCCACCGAGGGTATCTGAGACTCCCAGGCCCTGGTTGGTCCTGCCTCTTCTCC
  TTCATAGTGTCCCAGTGTTGTCAGGAGGGCGCTGGTGGTAGCTTGGACCTTGTGTGCCAACGCTTCCTCA
  GGTCTGGGCCTAACAGCCTCCACTGCCTGGGCTCACTCAGGGAGCGCCTCATTATTTGGGCAGCCATGGA
  TTCTATCCCAGCCCCATCATCAGTTCAGGGACACAACCTGACTGAAGATGCCAGACATCCTGAGAGTTGG
  CAGAACACAGGAGGCTATTCTGAAGGAGATGCAGTATCACAGCCACAGATGGCACTAGAGGAGGTGTCAG
  TGTCAGATCCACTGGCAAGCAACCAAGGACAGTCACTCCCAGGATCCTCAAGGGAGCACATGGCACAGTG
  GGAAGTGAGAAGCCAGACCCATGTTCCAAACAGAGAACCTGTTCAGGCACTGCCTTCCTCTGCCAGCCGG
  AAACGTCTGGACAAGAAACGTTCAGTGCCTGTAGCCACTGTAGAACTGGAAGAAAAGAGGTTCAGAACTC
  TGCCTTTAGTGCCAAGCCCCCTACAAGGCCTGACCAATCAGGATTTACAAGAGGGAGAAGATTGGGAGCA
  AGAAGATGAGGACATGGACCCCAGATTAGAACACAGTTCCTCAGTTCAAGAAGATTCTGAATCCCCAAGT
  CCTGAAGATATACCAGACTACCTCCTGCAATACAGGGCCATCCACAGTGCAGAACAGCAACATGCCTATG
  AGCAGGACTTTGAGACAGATTATGCTGAATACCGCATCCTGCATGCCCGTGTTGGGACTGCAAGCCAAAG
  GTTCATAGAGCTGGGAGCAGAGATTAAAAGAGTTCGGCGAGGAACTCCAGAATACAAGGTCCTGGAAGAC
  AAGATAATCCAGGAATATAAAAAGTTCAGGAAGCAGTACCCAAGTTACAGAGAAGAAAAGCGTCGCTGTG
  AGTACCTTCACCAGAAATTGTCCCACATTAAAGGTCTCATCCTGGAGTTTGAGGAAAAGAACAGGGGCAG
  C
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG200765 representing NM_025165
  Red=Cloning site Green=Tags(s)
  
  MEELQEPLRGQLRLCFTQAARTSLLLLRLNDAALRALQECQRQQVRPVIAFQGHRGYLRLPGPGWSCLFS
  FIVSQCCQEGAGGSLDLVCQRFLRSGPNSLHCLGSLRERLIIWAAMDSIPAPSSVQGHNLTEDARHPESW
  QNTGGYSEGDAVSQPQMALEEVSVSDPLASNQGQSLPGSSREHMAQWEVRSQTHVPNREPVQALPSSASR
  KRLDKKRSVPVATVELEEKRFRTLPLVPSPLQGLTNQDLQEGEDWEQEDEDMDPRLEHSSSVQEDSESPS
  PEDIPDYLLQYRAIHSAEQQHAYEQDFETDYAEYRILHARVGTASQRFIELGAEIKRVRRGTPEYKVLED
  KIIQEYKKFRKQYPSYREEKRRCEYLHQKLSHIKGLILEFEEKNRGS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025165
• ORF Size: 1191 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025165.3
• RefSeq Size: 2127 bp
• RefSeq ORF: 1194 bp
• Locus ID: 80237
• UniProt ID: Q9HB65
• Cytogenetics: 15q15.3
• Protein Families: Transcription Factors
• Gene Summary: Enhancer-binding elongation factor that specifically binds enhancers in embryonic stem cells (ES cells), marks them, and is required for their future activation during stem cell specification. Does not only bind to enhancer regions of active genes, but also marks the enhancers that are in a poised or inactive state in ES cells and is required for establishing proper RNA polymerase II occupancy at developmentally regulated genes in a cohesin-dependent manner. Probably required for priming developmentally regulated genes for later recruitment of the super elongation complex (SEC), for transcriptional activation during differentiation. Required for recruitment of P-TEFb within SEC during differentiation. Probably preloaded on germ cell chromatin, suggesting that it may prime gene activation by marking enhancers as early as in the germ cells. Promoting epithelial-mesenchymal transition (EMT) (By similarity). Elongation factor component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. Component of the little elongation complex (LEC), a complex required to regulate small nuclear RNA (snRNA) gene transcription by RNA polymerase II and III (PubMed:22195968).[UniProtKB/Swiss-Prot Function]