R Cadherin (CDH4) (NM_001794) Human Tagged ORF Clone

CAT#: RC210970

CDH4 (Myc-DDK-tagged)-Human cadherin 4, type 1, R-cadherin (retinal) (CDH4)

Product Images

Circular map for RC210970

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CDH4 (Cat# RC210970, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDH4 (Cat# TA804816). Positive lysates LY400684 (100ug) and LC400684 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY400684) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210970 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: R Cadherin
• Synonyms: CAD4; R-CAD; RCAD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210970 representing NM_001794
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCGCGGGCGCCGGCGTGCTCCTTCTGCTGCTCTCGCTCTCCGGCGCGCTCCGGGCCCATAATGAGG
  ATCTTACAACTAGAGAGACCTGCAAGGCTGGGTTCTCTGAAGATGATTACACGGCATTAATCTCCCAAAA
  TATTCTAGAAGGGGAAAAGCTACTTCAAGTCAAGTTCAGCAGCTGTGTGGGGACCAAGGGGACACAATAT
  GAGACCAACAGCATGGACTTCAAAGTTGGGGCAGATGGGACAGTCTTCGCCACCCGGGAGCTGCAGGTCC
  CCTCCGAGCAGGTGGCGTTCACGGTGACTGCATGGGACAGCCAGACAGCAGAGAAATGGGACGCCGTGGT
  GCGGTTGCTGGTGGCCCAGACCTCGTCCCCGCACTCTGGACACAAGCCGCAGAAAGGAAAGAAGGTCGTG
  GCTCTGGACCCCTCTCCGCCTCCGAAGGACACCCTGCTGCCGTGGCCCCAGCACCAGAACGCCAACGGGC
  TGAGGCGGCGCAAACGGGACTGGGTCATCCCGCCCATCAACGTGCCCGAGAACTCGCGCGGGCCCTTCCC
  GCAGCAGCTCGTGAGGATCCGGTCCGACAAAGACAATGACATCCCCATCCGGTACAGCATCACGGGAGTG
  GGCGCCGACCAGCCCCCCATGGAGGTCTTCAGCATTGACTCCATGTCCGGCCGGATGTACGTCACAAGGC
  CCATGGACCGGGAGGAGCACGCCTCTTACCACCTCCGAGCCCACGCTGTGGACATGAATGGCAACAAGGT
  GGAGAACCCCATCGACCTGTACATCTACGTCATCGACATGAATGACAACCGCCCTGAGTTCATCAACCAG
  GTCTACAACGGCTCCGTGGACGAGGGCTCCAAGCCAGGCACCTACGTGATGACCGTCACGGCCAACGATG
  CTGACGACAGCACCACGGCCAACGGGATGGTGCGGTACCGGATCGTGACCCAGACCCCACAGAGCCCGTC
  CCAGAATATGTTCACCATCAACAGCGAGACTGGAGATATCGTCACAGTGGCGGCTGGCCTGGACCGAGAG
  AAAGTTCAGCAGTACACAGTCATCGTTCAGGCCACAGATATGGAAGGAAATCTCAACTATGGCCTCTCAA
  ACACAGCCACAGCCATCATCACGGTGACAGATGTGAATGACAACCCGCCAGAATTTACCGCCAGCACGTT
  TGCAGGGGAGGTCCCCGAAAACCGCGTGGAGACCGTGGTCGCAAACCTCACGGTGATGGACCGAGATCAG
  CCCCACTCTCCAAACTGGAATGCCGTTTACCGCATCATCAGTGGGGATCCATCCGGGCACTTCAGCGTCC
  GCACAGATCCCGTAACCAACGAGGGCATGGTCACCGTGGTGAAGGCAGTCGACTACGAGCTCAACAGAGC
  TTTCATGCTGACAGTGATGGTGTCCAACCAGGCGCCCCTGGCCAGCGGAATCCAGATGTCCTTCCAGTCC
  ACGGCAGGGGTGACCATCTCCATCATGGACATCAACGAGGCTCCCTACTTCCCCTCAAACCACAAGCTGA
  TCCGCCTGGAGGAGGGCGTGCCCCCCGGCACCGTGCTGACCACGTTTTCAGCTGTGGACCCTGACCGGTT
  CATGCAGCAGGCTGTGAGATACTCAAAGCTGTCAGACCCAGCGAGCTGGCTGCACATCAATGCCACCAAC
  GGCCAGATCACCACGGCGGCAGTGCTGGACCGTGAGTCCCTCTACACCAAAAACAACGTCTACGAGGCCA
  CCTTCCTGGCAGCTGACAATGGGATACCCCCGGCCAGCGGCACCGGGACCCTCCAGATCTATCTCATTGA
  CATCAACGACAACGCCCCTGAGCTGCTGCCCAAGGAGGCGCAGATCTGCGAGAAGCCCAACCTGAACGCC
  ATCAACATCACGGCGGCCGACGCTGACGTCGACCCCAACATCGGCCCCTACGTCTTCGAGCTGCCCTTTG
  TCCCGGCGGCCGTGCGGAAGAACTGGACCATCACCCGCCTGAACGGTGACTATGCCCAACTCAGCTTGCG
  CATCCTGTACCTGGAGGCCGGGATGTATGACGTCCCCATCATCGTCACAGACTCTGGAAACCCTCCCCTG
  TCCAACACGTCCATCATCAAAGTCAAGGTGTGCCCATGTGATGACAACGGGGACTGCACCACCATTGGCG
  CAGTGGCAGCGGCTGGTCTGGGCACCGGTGCCATCGTGGCCATCCTCATCTGCATCCTCATCCTGCTGAC
  CATGGTCCTGCTGTTTGTCATGTGGATGAAGCGGCGAGAGAAGGAGCGCCACACGAAGCAGCTGCTCATT
  GACCCCGAGGACGACGTCCGCGACAACATCCTCAAGTATGACGAGGAAGGCGGTGGCGAGGAGGACCAGG
  ACTACGACCTCAGCCAGCTGCAGCAGCCGGAAGCCATGGGGCACGTGCCAAGCAAAGCCCCTGGCGTGCG
  TCGCGTGGATGAGCGGCCGGTGGGCGCTGAGCCCCAGTACCCGATCAGGCCCATGGTGCCGCACCCAGGC
  GACATCGGTGACTTCATCAATGAGGGACTCCGCGCTGCTGACAACGACCCCACGGCACCCCCCTATGACT
  CCCTGCTGGTCTTCGACTACGAGGGGAGCGGCTCCACCGCAGGCTCCGTCAGCTCCCTGAACTCATCCAG
  TTCCGGGGACCAAGACTACGATTACCTCAACGACTGGGGGCCCAGATTCAAGAAGCTGGCGGACATGTAT
  GGAGGTGGTGAAGAGGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210970 representing NM_001794
  Red=Cloning site Green=Tags(s)
  
  MTAGAGVLLLLLSLSGALRAHNEDLTTRETCKAGFSEDDYTALISQNILEGEKLLQVKFSSCVGTKGTQY
  ETNSMDFKVGADGTVFATRELQVPSEQVAFTVTAWDSQTAEKWDAVVRLLVAQTSSPHSGHKPQKGKKVV
  ALDPSPPPKDTLLPWPQHQNANGLRRRKRDWVIPPINVPENSRGPFPQQLVRIRSDKDNDIPIRYSITGV
  GADQPPMEVFSIDSMSGRMYVTRPMDREEHASYHLRAHAVDMNGNKVENPIDLYIYVIDMNDNRPEFINQ
  VYNGSVDEGSKPGTYVMTVTANDADDSTTANGMVRYRIVTQTPQSPSQNMFTINSETGDIVTVAAGLDRE
  KVQQYTVIVQATDMEGNLNYGLSNTATAIITVTDVNDNPPEFTASTFAGEVPENRVETVVANLTVMDRDQ
  PHSPNWNAVYRIISGDPSGHFSVRTDPVTNEGMVTVVKAVDYELNRAFMLTVMVSNQAPLASGIQMSFQS
  TAGVTISIMDINEAPYFPSNHKLIRLEEGVPPGTVLTTFSAVDPDRFMQQAVRYSKLSDPASWLHINATN
  GQITTAAVLDRESLYTKNNVYEATFLAADNGIPPASGTGTLQIYLIDINDNAPELLPKEAQICEKPNLNA
  INITAADADVDPNIGPYVFELPFVPAAVRKNWTITRLNGDYAQLSLRILYLEAGMYDVPIIVTDSGNPPL
  SNTSIIKVKVCPCDDNGDCTTIGAVAAAGLGTGAIVAILICILILLTMVLLFVMWMKRREKERHTKQLLI
  DPEDDVRDNILKYDEEGGGEEDQDYDLSQLQQPEAMGHVPSKAPGVRRVDERPVGAEPQYPIRPMVPHPG
  DIGDFINEGLRAADNDPTAPPYDSLLVFDYEGSGSTAGSVSSLNSSSSGDQDYDYLNDWGPRFKKLADMY
  GGGEED
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001794
• ORF Size: 2748 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001794.5
• RefSeq Size: 3063 bp
• RefSeq ORF: 2751 bp
• Locus ID: 1002
• UniProt ID: P55283
• Cytogenetics: 20q13.33
• Domains: Cadherin_C_term, CA
• Protein Families: Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs)
• MW: 100.28 kDa
• Gene Summary: This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Based on studies in chicken and mouse, this cadherin is thought to play an important role during brain segmentation and neuronal outgrowth. In addition, a role in kidney and muscle development is indicated. Of particular interest are studies showing stable cis-heterodimers of cadherins 2 and 4 in cotransfected cell lines. Previously thought to interact in an exclusively homophilic manner, this is the first evidence of cadherin heterodimerization. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]