OriGene - MYLK3 (NM_182493) Human ORF cDNA Clone

Western Blot Validated

The same insert cloned in a GFP-tagging vector is also available

Cat. No.	Description	Vector	Price	Availability
RG222246	GFP-tagged ORF clone of Homo sapiens myosin light chain kinase 3 (MYLK3) as transfection-ready DNA	pCMV6-AC-GFP	$590	In Stock
TA150041	2H8, Anti-tGFP monoclonal antibody, 100µl		$248	In Stock

TrueORF Data for RC222246

Vector:	pCMV6-Entry Change vector?	Tag: C-terminal Myc-DDK	Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Sequence Data:	ORF Nucleotide Sequence CHROMATOGRAMS Protein Sequence	ORF Size: 2460 bp Predicted Protein MW: 88.2 kDa
Restriction Sites:	SgfI-MluI Cloning Scheme for this gene Plasmid Map
OTI Annotation:	This clone was engineered to express the complete ORF with an expression tag.
OTI Disclaimer:	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components:	The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Protein Families:	Protein Kinase Druggable Genome
Protein Pathways:	Calcium signaling pathway Vascular smooth muscle contraction Focal adhesion Regulation of actin cytoskeleton

* The lysates used for this WB picture contain the overexpressed empty vector or the Myc-DDK tagged ORF clone.

Reference Data

RefSeq: NM_182493.2, NP_872299	RefSeq Size: 7998	RefSeq ORF: 2460
Synonyms : caMLCK; MLCK; MLCK2
LocusID: 91807	Cytogenetic: 16q11.2
Summary: Phosphorylation of cardiac myosin heavy chains (see MYH7B, MIM 609928) and light chains (see MYL2, MIM 160781) by a kinase, such as MYLK3, potentiates the force and rate of cross-bridge recruitment in cardiac myocytes (Chan et al., 2008 [PubMed 18202317]).[supplied by OMIM, Jul 2008].

ORF Clones Citations:

Solute Carrier 11A1 Is Expressed by Innate Lymphocytes and Augments Their Activation, Jodi F. Hedges, Emily Kimmel, Deann T. Snyder, Maria Jerome, and Mark A. Jutila, J. Immunol., Apr 2013; 190: 4263 - 4273. [SLC11A1]

A common polymorphism in the LDL receptor gene has multiple effects on LDL receptor function, Feng Gao, Hansel E. Ihn, Marisa W. Medina, and Ronald M. Krauss, Hum. Mol. Genet., Apr 2013; 22: 1424 - 1431. [LDLR]

RNA elements directing in vivo assembly of the 7SK/MePCE/Larp7 transcriptional regulatory snRNP, Lisa Muniz, Sylvain Egloff, and Tamás Kiss, Nucleic Acids Res., Apr 2013; 41: 4686 - 4698. [MEPCE ]

Structural basis for angiopoietin-1–mediated signaling initiation, Xuehong Yu, Tom C. M. Seegar, Annamarie C. Dalton, Dorothea Tzvetkova-Robev, Yehuda Goldgur, Kanagalaghatta R. Rajashankar, Dimitar B. Nikolov, and William A. Barton, PNAS, Apr 2013; 110: 7205 - 7210. [ANGPT1]

Inverted Formin 2 Regulates Actin Dynamics by Antagonizing Rho/Diaphanous-related Formin Signaling, Hua Sun, Johannes Schlondorff, Henry N. Higgs, and Martin R. Pollak, J. Am. Soc. Nephrol., Apr 2013; 10.1681/ASN.2012080834. [INF2]

MASL1 induces erythroid differentiation in human erythropoietin-dependent CD34+ cells through the Raf/MEK/ERK pathway, Chutima Kumkhaek, Wulin Aerbajinai, Wenli Liu, Jianqiong Zhu, Naoya Uchida, Roger Kurlander, Matthew M. Hsieh, John F. Tisdale, and Griffin P. Rodgers, Blood, Apr 2013; 121: 3216 - 3227. [MFHAS1 ]

Proximal tubule-dominant transfer of AT1a receptors induces blood pressure responses to intracellular angiotensin II in AT1a receptor-deficient mice, Xiao C. Li and Jia L. Zhuo, Am J Physiol Regulatory Integrative Comp Physiol, Apr 2013; 304: R588 - R598. [AGTR1A ]

DCNL1 Functions as a Substrate Sensor and Activator of Cullin 2-RING Ligase, Pardeep Heir, Roxana I. Sufan, Samantha N. Greer, Betty P. Poon, Jeffrey E. Lee, and Michael Ohh, Mol. Cell. Biol., Apr 2013; 33: 1621 - 1631. [DCNL1]

More TrueClone Citations >>

MYLK3 Mouse Clones

SKU	Description	Amount	Price	Shipping
MC221728	Mus musculus myosin light chain kinase 3 (Mylk3) as transfection-ready DNA	10 ug	$960	6 weeks
MR225821	Myc-DDK-tagged ORF clone of Mus musculus myosin light chain kinase 3 (Mylk3) as transfection-ready DNA	10 ug	$960	6 weeks
MG225821	GFP-tagged ORF clone of Mus musculus myosin light chain kinase 3 (Mylk3) as transfection-ready DNA	10 ug	$1060	3 weeks

MYLK3 Rat Clones

SKU	Description	Amount	Price	Shipping
RN211117	NativeORF clone of Rattus norvegicus myosin light chain kinase 3 (Mylk3) as transfection-ready DNA	10 ug	$944	6 weeks
RR211117	Myc-DDK-tagged ORF clone of Rattus norvegicus myosin light chain kinase 3 (Mylk3) as transfection-ready DNA	10 ug	$944	6 weeks

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MYLK3 (NM_182493) Human cDNA ORF Clone

Western Blot Validated

ORF Clones Citations:

MYLK3 Mouse Clones

MYLK3 Rat Clones

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