CCBL1 (KYAT1) (NM_004059) Human Tagged ORF Clone

CAT#: RC216317

CCBL1 (Myc-DDK-tagged)-Human cysteine conjugate-beta lyase, cytoplasmic (CCBL1), transcript variant 1

Product Images

Circular map for RC216317

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  Western blot validation of overexpression lysate (Cat# LY426545) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC225677 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified KYAT1 protein (Cat# TP316317). The protein was produced from HEK293T cells transfected with KYAT1 cDNA clone (Cat# RC216317) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CCBL1
• Synonyms: CCBL1; GTK; KAT1; KATI
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216317 representing NM_004059
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAAACAGCTGCAGGCCCGAAGGCTAGACGGGATCGACTACAACCCCTGGGTGGAGTTTGTGAAAC
  TGGCCAGTGAGCATGACGTCGTGAACTTGGGCCAGGGCTTCCCGGATTTCCCACCACCAGACTTTGCCGT
  GGAAGCCTTTCAGCACGCTGTCAGTGGAGACTTCATGCTTAACCAGTACACCAAGACATTTGGTTACCCA
  CCACTGACGAAGATCCTGGCAAGTTTCTTTGGGGAGCTGCTGGGTCAGGAGATAGACCCGCTCAGGAATG
  TGCTGGTGACTGTTGGTGGCTATGGGGCCCTGTTCACAGCCTTCCAGGCCCTGGTGGACGAAGGAGACGA
  GGTCATCATCATCGAACCCTTTTTTGACTGCTACGAGCCCATGACAATGATGGCAGGGGGTCGTCCTGTG
  TTTGTGTCCCTGAAGCCGGGTCCCATCCAGAATGGAGAACTGGGTTCCAGCAGCAACTGGCAGCTGGACC
  CCATGGAGCTGGCCGGCAAATTCACATCACGCACCAAAGCCCTGGTCCTCAACACCCCCAACAACCCCCT
  GGGCAAGGTGTTCTCCAGGGAAGAGCTGGAGCTGGTGGCCAGCCTTTGCCAGCAGCATGACGTGGTGTGT
  ATCACTGATGAAGTCTACCAGTGGATGGTCTACGACGGGCACCAGCACATCAGCATTGCCAGCCTCCCTG
  GCATGTGGGAACGGACCCTGACCATCGGCAGCGCCGGCAAGACCTTCAGCGCCACTGGCTGGAAGGTGGG
  CTGGGTCCTGGGTCCAGATCACATCATGAAGCACCTGCGGACCGTGCACCAGAACTCCGTCTTCCACTGC
  CCCACGCAGAGCCAGGCTGCAGTAGCCGAGAGCTTTGAACGGGAGCAGCTGCTCTTCCGCCAACCCAGCA
  GCTACTTTGTGCAGTTCCCGCAGGCCATGCAGCGCTGCCGTGACCACATGATACGTAGCCTACAGTCAGT
  GGGCCTGAAGCCCATCATCCCTCAGGGCAGCTACTTCCTCATCACAGACATCTCAGACTTCAAGAGGAAG
  ATGCCTGACTTGCCTGGAGCTGTGGATGAGCCCTATGACAGACGCTTCGTCAAGTGGATGATCAAGAACA
  AGGGCTTGGTGGCCATCCCTGTCTCCATCTTCTATAGTGTGCCACATCAGAAGCACTTTGACCACTATAT
  CCGCTTCTGTTTTGTGAAGGATGAAGCCACGCTCCAGGCCATGGACGAGAAGCTGCGGAAGTGGAAGGTG
  GAACTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216317 representing NM_004059
  Red=Cloning site Green=Tags(s)
  
  MAKQLQARRLDGIDYNPWVEFVKLASEHDVVNLGQGFPDFPPPDFAVEAFQHAVSGDFMLNQYTKTFGYP
  PLTKILASFFGELLGQEIDPLRNVLVTVGGYGALFTAFQALVDEGDEVIIIEPFFDCYEPMTMMAGGRPV
  FVSLKPGPIQNGELGSSSNWQLDPMELAGKFTSRTKALVLNTPNNPLGKVFSREELELVASLCQQHDVVC
  ITDEVYQWMVYDGHQHISIASLPGMWERTLTIGSAGKTFSATGWKVGWVLGPDHIMKHLRTVHQNSVFHC
  PTQSQAAVAESFEREQLLFRQPSSYFVQFPQAMQRCRDHMIRSLQSVGLKPIIPQGSYFLITDISDFKRK
  MPDLPGAVDEPYDRRFVKWMIKNKGLVAIPVSIFYSVPHQKHFDHYIRFCFVKDEATLQAMDEKLRKWKV
  EL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004059
• ORF Size: 1266 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004059.5
• RefSeq Size: 1925 bp
• RefSeq ORF: 1269 bp
• Locus ID: 883
• UniProt ID: Q16773
• Cytogenetics: 9q34.11
• Domains: aminotran_1_2
• MW: 47.7 kDa
• Gene Summary: This gene encodes a cytosolic enzyme that is responsible for the metabolism of cysteine conjugates of certain halogenated alkenes and alkanes. This metabolism can form reactive metabolites leading to nephrotoxicity and neurotoxicity. Increased levels of this enzyme have been linked to schizophrenia. Multiple transcript variants that encode different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]