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Home cDNA Clone TrueORF All VAC14 ORF Clones
TrueORF Gold

VAC14 (NM_018052) Human cDNA ORF Clone

Specifications Citations Clones of Other Species Product Documents
Cat. No. Description Price Availability  
RC214195 VAC14 (Myc-DDK-tagged)-Human Vac14 homolog (S. cerevisiae) (VAC14), 10µg
1-2 weeks
RC514195 VAC14 (Myc-DDK-tagged)-Human Vac14 homolog (S. cerevisiae) (VAC14), (200ug) $660 3 weeks
TA50011-100 4C5, Anti-DDK monoclonal antibody, 100µl $248 In Stock
Clone Modification

The same insert cloned in Lenti and other vectors is also available.

Cat. No. Description Vector Price Availability  
RC214195L1 Lenti ORF clone of Human Vac14 homolog (S. cerevisiae) (VAC14) , Myc-DDK-tagged

$910 In Stock
TR30022 Lenti-vpak packaging kit - packaging plasmids and transfection reagent (10 rxns) Lenti-vpak-app-guide $390 In Stock
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TrueORF Data for RC214195
Vector: pCMV6-Entry   Change vector? Tag: C-terminal Myc-DDK
Western validation with an anti-DDK antibody *
L: Control HEK293 lysate
R: Over-expression lysate
Sequence Data: ORF Nucleotide Sequence
Protein Sequence
ORF Size: 2349 bp
Predicted Protein MW: 87.8 kDa
Restriction Sites: SgfI-MluI     Cloning Scheme for this gene     Plasmid Map Plasmid Map
OTI Annotation: This clone was engineered to express the complete ORF with an expression tag.
OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
* The lysates used for this WB picture contain the overexpressed empty vector or the Myc-DDK tagged ORF clone.

Reference Data
RefSeq: NM_018052.3, NP_060522
RefSeq Size: 3107 RefSeq ORF: 2349
Synonyms : ArPIKfyve; TAX1BP2; TRX
LocusID: 55697 Cytogenetic: 16q22.1
Summary: The content of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) in endosomal membranes changes dynamically with fission and fusion events that generate or absorb intracellular transport vesicles. VAC14 is a component of a trimolecular complex that tightly regulates the level of PtdIns(3,5)P2. Other components of this complex are the PtdIns(3,5)P2-synthesizing enzyme PIKFYVE (MIM 609414) and the PtdIns(3,5)P2 phosphatase FIG4 (MIM 609390). VAC14 functions as an activator of PIKFYVE (Sbrissa et al., 2007 [PubMed 17556371]).[supplied by OMIM, Feb 2010]. ##Evidence-Data-START## Transcript exon combination :: AK056433.1, BC125108.1 [ECO:0000332] RNAseq introns :: single sample supports all introns ERS025087 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.


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