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Home cDNA Clone TrueORF All LIF ORF Clones
TrueORF Gold

LIF (NM_002309) Human cDNA ORF Clone

Specifications Citations Clones of Other Species Product Documents
Cat. No. Description Price Availability  
RC210818 LIF (Myc-DDK-tagged)-Human leukemia inhibitory factor (cholinergic differentiation factor) (LIF), 10µg
$480
In Stock
RC510818 LIF (Myc-DDK-tagged)-Human leukemia inhibitory factor (cholinergic differentiation factor) (LIF), (200ug) $500 2~3 weeks
TA50011-100 4C5, Anti-DDK monoclonal antibody, 100µl $248 In Stock
Clone Modification

The same insert cloned in Lenti and other vectors is also available.

Cat. No. Description Vector Price Availability  
RC210818L2 Lenti ORF clone of Human leukemia inhibitory factor (cholinergic differentiation factor) (LIF), mGFP tagged

$830 2 Weeks
TR30022 Lenti-vpak packaging kit - packaging plasmids and transfection reagent (10 rxns) Lenti-vpak-app-guide $390 In Stock
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Also for LIF (NM_002309)
cDNA Clone shRNA/siRNA Lysate Protein Antibody
TrueORF Data for RC210818
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Vector: pCMV6-Entry   Change vector? Tag: C-terminal Myc-DDK
Western validation with an anti-DDK antibody *
L: Control HEK293 lysate
R: Over-expression lysate
Lysate_Image
Sequence Data: ORF Nucleotide Sequence
Protein Sequence
ORF Size: 609 bp
Predicted Protein MW: 19.7 kDa
Restriction Sites: SgfI-MluI     Cloning Scheme for this gene     Plasmid Map Plasmid Map
OTI Annotation: This clone was engineered to express the complete ORF with an expression tag.
OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Protein Families: Secreted ProteinES Cell Differentiation/IPSAdult stem cellsDruggable Genome
Protein Pathways: Cytokine-cytokine receptor interactionJak-STAT signaling pathway
* The lysates used for this WB picture contain the overexpressed empty vector or the Myc-DDK tagged ORF clone.

Reference Data
RefSeq: NM_002309.2, NP_002300
RefSeq Size: 3868 RefSeq ORF: 609
Synonyms : CDF; DIA; HILDA; MLPLI
LocusID: 3976 Cytogenetic: 22q12.2
Summary: The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012].

 

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