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Home cDNA Clone TrueORF All MAP3K5 ORF Clones

MAP3K5 (NM_005923) Human cDNA ORF Clone

Western Blot Validated

Specifications Citations Clones of Other Species Product Documents
Cat. No. Description Price Availability  
RC209913 MAP3K5 (Myc-DDK-tagged)-Human mitogen-activated protein kinase kinase kinase 5 (MAP3K5), 10µg
$780
1-2 weeks
TA50011-100 4C5, Anti-DDK monoclonal antibody, 100µl $248 In Stock
Clone Modification

The same insert cloned in Lenti and other vectors is also available.

Cat. No. Description Vector Price Availability  
RC209913L2 Lenti-ORF clone of MAP3K5 (mGFP-tagged)-Human mitogen-activated protein kinase kinase kinase 5 (MAP3K5), (10ug)

1270 4 Weeks
TR30022 Lenti-vpak packaging kit - packaging plasmids and transfection reagent (10 rxns) Lenti-vpak-app-guide $390 In Stock
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Also for MAP3K5 (NM_005923)
cDNA Clone shRNA/siRNA Lysate Protein Antibody
TrueORF Data for RC209913
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Vector: pCMV6-Entry   Change vector? Tag: C-terminal Myc-DDK
Western validation with an anti-DDK antibody *
L: Control HEK293 lysate
R: Over-expression lysate
Lysate_Image
Sequence Data: ORF Nucleotide Sequence
Protein Sequence
ORF Size: 4125 bp
Predicted Protein MW: 154.4 kDa
Restriction Sites: SgfI-MluI     Cloning Scheme for this gene     Plasmid Map Plasmid Map
OTI Annotation: This clone was engineered to express the complete ORF with an expression tag.
OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Protein Families: Protein KinaseDruggable Genome
Protein Pathways: MAPK signaling pathwayNeurotrophin signaling pathwayAmyotrophic lateral sclerosis (ALS)

Reference Data
RefSeq: NM_005923.3, NP_005914
RefSeq Size: 5215 RefSeq ORF: 4125
Synonyms : ASK1; MAPKKK5; MEKK5
LocusID: 4217 Cytogenetic: 6q22.33 Domains: pkinase, TyrKc, S_TKc
Summary: Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK. [provided by RefSeq, Jul 2008].

 

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