OriGene - MAPK10 (NM_002753) Human ORF cDNA Clone

Western Blot Validated

The same insert cloned in a GFP-tagging vector is also available

Cat. No.	Description	Vector	Price	Availability
RG207216	GFP-tagged ORF clone of Homo sapiens mitogen-activated protein kinase 10 (MAPK10), transcript variant 1 as transfection-ready DNA	pCMV6-AC-GFP	$590	In Stock
TA150041	2H8, Anti-tGFP monoclonal antibody, 100µl		$248	In Stock

TrueORF Data for RC207216

Vector:	pCMV6-Entry Change vector?	Tag: C-terminal Myc-DDK	Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Sequence Data:	ORF Nucleotide Sequence CHROMATOGRAMS Protein Sequence	ORF Size: 1269 bp Predicted Protein MW: 48.4 kDa
Restriction Sites:	SgfI-MluI Cloning Scheme for this gene Plasmid Map
OTI Annotation:	This clone was engineered to express the complete ORF with an expression tag.
OTI Disclaimer:	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components:	The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Protein Families:	Protein Kinase Druggable Genome
Protein Pathways:	MAPK signaling pathway ErbB signaling pathway Wnt signaling pathway Focal adhesion Toll-like receptor signaling pathway NOD-like receptor signaling pathway More Pathways >>

* The lysates used for this WB picture contain the overexpressed empty vector or the Myc-DDK tagged ORF clone.

Reference Data

RefSeq: NM_002753.3, NP_002744	RefSeq Size: 2372	RefSeq ORF: 1269
Synonyms : JNK3; JNK3A; p493F12; p54bSAPK; PRKM10; SAPK1b
LocusID: 5602	Cytogenetic: 4q22.1	Domains: pkinase, TyrKc, S_TKc
Summary: The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This protein is a neuronal-specific form of c-Jun N-terminal kinases (JNKs). Through its phosphorylation and nuclear localization, this kinase plays regulatory roles in the signaling pathways during neuronal apoptosis. Beta-arrestin 2, a receptor-regulated MAP kinase scaffold protein, is found to interact with, and stimulate the phosphorylation of this kinase by MAP kinase kinase 4 (MKK4). Cyclin-dependent kianse 5 can phosphorylate, and inhibit the activity of this kinase, which may be important in preventing neuronal apoptosis. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008].

ORF Clones Citations:

Intracellular Trafficking of the KV1.3 Potassium Channel Is Regulated by the Prodomain of a Matrix Metalloprotease, Hai M. Nguyen, Charles A. Galea, Galina Schmunk, Brian J. Smith, Robert A. Edwards, Raymond S. Norton, and K. George Chandy, J. Biol. Chem., Mar 2013; 288: 6451 - 6464. [KV1.3]

Renal Hypodysplasia Associates with a Wnt4 Variant that Causes Aberrant Canonical Wnt Signaling, Asaf Vivante, Michal Mark-Danieli, Miriam Davidovits, Orit Harari-Steinberg, Dorit Omer, Yehudit Gnatek, Roxana Cleper, Daniel Landau, Yael Kovalski, Irit Weissman, Israel Eisenstein, Michalle Soudack, Haike Reznik Wolf, Naomi Issler, Danny Lotan, Yair Anikster, and Benjamin Dekel, J. Am. Soc. Nephrol., Mar 2013; 24: 550 - 558. [WNT4]

Smad4-Irf6 genetic interaction and TGFß-mediated IRF6 signaling cascade are crucial for palatal fusion in mice, Jun-ichi Iwata, Akiko Suzuki, Richard C. Pelikan, Thach-Vu Ho, Pedro A. Sanchez-Lara, Mark Urata, Michael J. Dixon, and Yang Chai, Development, Mar 2013; 140: 1220 - 1230. [p21]

Mutation of the iron-sulfur cluster assembly gene IBA57 causes severe myopathy and encephalopathy, Nikhita Ajit Bolar, Arnaud Vincent Vanlander, Claudia Wilbrecht, Nathalie Van der Aa, Joél Smet, Boel De Paepe, Geert Vandeweyer, Frank Kooy, François Eyskens, Elien De Latter, Gwenda Delanghe, Paul Govaert, Jules Gerard Leroy, Bart Loeys, Roland Lill, Lut Van Laer, and Rudy Van Coster, Hum. Mol. Genet., Mar 2013; 10.1093/hmg/ddt107. [IBA57]

Mutations in Critical Domains Confer the Human mTOR Gene Strong Tumorigenicity, Avaniyapuram Kannan Murugan, Ali Alzahrani, and Mingzhao Xing, J. Biol. Chem., Mar 2013; 288: 6511 - 6521. [mTOR]

Specificity Profiling of Dual Specificity Phosphatase Vaccinia VH1-related (VHR) Reveals Two Distinct Substrate Binding Modes, Rinrada Luechapanichkul, Xianwen Chen, Hashem A. Taha, Shubham Vyas, Xiaoyan Guan, Michael A. Freitas, Christopher M. Hadad, and Dehua Pei, J. Biol. Chem., Mar 2013; 288: 6498 - 6510. [ROBO1]

Dissecting the Role of COPI Complexes in Influenza Virus Infection, Eileen Sun, Jiang He, and Xiaowei Zhuang, J. Virol., Mar 2013; 87: 2673 - 2685. [COPE]

ATF4 Protects Against Neuronal Death in Cellular Parkinson's Disease Models by Maintaining Levels of Parkin, Xiaotian Sun, Jin Liu, John F. Crary, Cristina Malagelada, David Sulzer, Lloyd A. Greene, and Oren A. Levy, J. Neurosci., Feb 2013; 33: 2398 - 2407. [Myc-tagged rat parkin]

Functional analysis of sucrase–isomaltase mutations from chronic lymphocytic leukemia patients, David Rodríguez, Andrew J. Ramsay, Víctor Quesada, Cecilia Garabaya, Elías Campo, José M. P. Freije, and Carlos López-Otín, Hum. Mol. Genet., Feb 2013; 10.1093/hmg/ddt078. [SI]

Mutation of the ATP-gated P2X2 receptor leads to progressive hearing loss and increased susceptibility to noise, Denise Yan, Yan Zhu, Tom Walsh, Dinghua Xie, Huijun Yuan, Asli Sirmaci, Taro Fujikawa, Ann Chi Yan Wong, Tze L. Loh, Lilin Du, M’hamed Grati, Srdjan M. Vlajkovic, Susan Blanton, Allen F. Ryan, Zheng-Yi Chen, Peter R. Thorne, Bechara Kachar, Mustafa Tekin, Hong-Bo Zhao, Gary D. Housley, Mary-Claire King, and Xue Z. Liu, PNAS, Feb 2013; 110: 2228 - 2233. [P2RX2 ]

More TrueClone Citations >>

MAPK10 Mouse Clones

SKU	Description	Amount	Price	Shipping
MC209721	Mus musculus mitogen-activated protein kinase 10 (Mapk10), transcript variant 1 as transfection-ready DNA	10 ug	$380	3 weeks
MC216295	Mus musculus mitogen-activated protein kinase 10 (Mapk10), transcript variant 2 as transfection-ready DNA	10 ug	$560	4 weeks
MR206714	Myc-DDK-tagged ORF clone of Mus musculus mitogen-activated protein kinase 10 (Mapk10), transcript variant 2 as transfection-ready DNA	10 ug	$560	4 weeks
MR226312	Myc-DDK-tagged ORF clone of Mus musculus mitogen-activated protein kinase 10 (Mapk10), transcript variant 1 as transfection-ready DNA	10 ug	$380	3 weeks
MG226312	GFP-tagged ORF clone of Mus musculus mitogen-activated protein kinase 10 (Mapk10) transcript variant 1 as transfection-ready DNA	10 ug	$420	3 weeks
MG206714	GFP-tagged ORF clone of Mus musculus mitogen activated protein kinase 10 (Mapk10), transcript variant 1 in a GFP expression vector	10 ug	$620	3 weeks

MAPK10 Rat Clones

SKU	Description	Amount	Price	Shipping
RN203224	NativeORF clone of Rattus norvegicus mitogen activated protein kinase 10 (Mapk10) as transfection-ready DNA	10 ug	$380	3 weeks
RR203224	Myc-DDK-tagged ORF clone of Rattus norvegicus mitogen activated protein kinase 10 (Mapk10) as transfection-ready DNA	10 ug	$380	Next day

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MAPK10 (NM_002753) Human cDNA ORF Clone

Western Blot Validated

ORF Clones Citations:

MAPK10 Mouse Clones

MAPK10 Rat Clones

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