hCG (CGA) (NM_000735) Human Tagged ORF Clone
CAT#: RC203146
CGA (Myc-DDK-tagged)-Human glycoprotein hormones, alpha polypeptide (CGA)
ORF Plasmid: tGFP
Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro
AAV Particle: DDK
"NM_000735" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | hCG |
Synonyms | CG-ALPHA; FSHA; GPA1; GPHa; GPHA1; HCG; LHA; TSHA |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC203146 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATTACTACAGAAAATATGCAGCTATCTTTCTGGTCACATTGTCGGTGTTTCTGCATGTTCTCCATT CCGCTCCTGATGTGCAGGATTGCCCAGAATGCACGCTACAGGAAAACCCACTCTTCTCCCAGCCGGGTGC CCCAATACTTCAGTGCATGGGCTGCTGCTTCTCTAGAGCATATCCCACTCCACTAAGGTCCAAGAAGACG ATGTTGGTCCAAAAGAACGTCACCTCAGAGTCCACTTGCTGTGTAGCTAAATCATATAACAGGGTCACAG TAATGGGGGGTTTCAAAGTGGAGAACCACACGGCGTGCCACTGCAGTACTTGTTATTATCACAAATCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203146 protein sequence
Red=Cloning site Green=Tags(s) MDYYRKYAAIFLVTLSVFLHVLHSAPDVQDCPECTLQENPLFSQPGAPILQCMGCCFSRAYPTPLRSKKT MLVQKNVTSESTCCVAKSYNRVTVMGGFKVENHTACHCSTCYYHKS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_000735 |
ORF Size | 348 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_000735.4 |
RefSeq Size | 768 bp |
RefSeq ORF | 351 bp |
Locus ID | 1081 |
UniProt ID | P01215 |
Cytogenetics | 6q14.3 |
Domains | GHA |
Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein |
Protein Pathways | Autoimmune thyroid disease, GnRH signaling pathway, Neuroactive ligand-receptor interaction |
MW | 13 kDa |
Gene Summary | The four human glycoprotein hormones chorionic gonadotropin (CG), luteinizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH) are dimers consisting of alpha and beta subunits that are associated noncovalently. The alpha subunits of these hormones are identical, however, their beta chains are unique and confer biological specificity. The protein encoded by this gene is the alpha subunit and belongs to the glycoprotein hormones alpha chain family. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC203146L1 | Lenti ORF clone of Human glycoprotein hormones, alpha polypeptide (CGA), Myc-DDK-tagged |
USD 450.00 |
|
RC203146L2 | Lenti ORF clone of Human glycoprotein hormones, alpha polypeptide (CGA), mGFP tagged |
USD 450.00 |
|
RC203146L3 | Lenti ORF clone of Human glycoprotein hormones, alpha polypeptide (CGA), Myc-DDK-tagged |
USD 450.00 |
|
RC203146L4 | Lenti ORF clone of Human glycoprotein hormones, alpha polypeptide (CGA), mGFP tagged |
USD 450.00 |
|
RG203146 | CGA (tGFP-tagged) - Human glycoprotein hormones, alpha polypeptide (CGA) |
USD 350.00 |
|
SC119734 | CGA (untagged)-Human glycoprotein hormones, alpha polypeptide (CGA) |
USD 150.00 |
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