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Home cDNA Clone TrueORF All DBT ORF Clones
TrueORF Gold

DBT (NM_001918) Human cDNA ORF Clone

Specifications Citations Clones of Other Species Product Documents
Cat. No. Description Price Availability  
RC201998 DBT (Myc-DDK-tagged)-Human dihydrolipoamide branched chain transacylase E2 (DBT), nuclear gene encoding mitochondrial protein, 10µg   
$600
In Stock
RG201998 DBT (GFP-tagged) - Human dihydrolipoamide branched chain transacylase E2 (DBT), nuclear gene encoding mitochondrial protein $660 In Stock
TA50011-100 4C5, Anti-DDK monoclonal antibody, 100µl $248 In Stock
Cat. No. Description Price Availability
RC501998 DBT (Myc-DDK-tagged)-Human dihydrolipoamide branched chain transacylase E2 (DBT), nuclear gene encoding mitochondrial protein, (200ug) $720 2 weeks
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The same insert cloned in a GFP-tagging vector is also available

Cat. No. Description Vector Price Availability  
RG201998 DBT (GFP-tagged) - Human dihydrolipoamide branched chain transacylase E2 (DBT), nuclear gene encoding mitochondrial protein

$660 In Stock
TA150041 2H8, Anti-tGFP monoclonal antibody, 100µl Datasheet $248 In Stock
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TrueORF Data for RC201998
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Vector: pCMV6-Entry   Change vector? Tag: C-terminal Myc-DDK
Western validation with an anti-DDK antibody *
L: Control HEK293 lysate
R: Over-expression lysate
Lysate_Image
Sequence Data: ORF Nucleotide Sequence
Protein Sequence
ORF Size: 1446 bp
Predicted Protein MW: 53.49 kDa
Restriction Sites: SgfI-MluI     Cloning Scheme for this gene     Plasmid Map Plasmid Map
OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Protein Families: Druggable Genome
Protein Pathways: Valine, leucine and isoleucine degradationMetabolic pathways
* The lysates used for this WB picture contain the overexpressed empty vector or the Myc-DDK tagged ORF clone.

Reference Data
RefSeq Explanation: NM_001918.1 NM_001918.2 NM_001918.3 , NP_001909
Synonyms: BCATE2; BCKAD-E2; BCKADE2; BCOADC-E2; E2; E2B RefSeq Size: 10831 RefSeq ORF: 1449
LocusID: 1629 Cytogenetic: 1p21.2 Domains: biotin_lipoyl, 2-oxoacid_dh, e3_binding
Gene Summary: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3). Within this complex, the catalytic function of this enzyme is to accept, and to transfer to coenzyme A, acyl groups that are generated by the branched-chain alpha-keto acid decarboxylase component. [UniProtKB/Swiss-Prot Function]

 

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