OriGene - NDUFA5 (NM_005000) Human ORF cDNA Clone

Western Blot Validated

The same insert cloned in Lenti and other vectors is also available.

Cat. No.	Description	Vector	Price	Availability
RC201539L1	Lenti ORF clone of Human NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13kDa (NDUFA5), nuclear gene encoding mitochondrial protein , Myc-DDK-tagged	pLenti-C-Myc-DDK pLenti-C-mGFP pCMV6-AC-GFP	$750	2 Weeks
TR30022	Lenti-vpak packaging kit - packaging plasmids and transfection reagent (10 rxns)		$360	In Stock

TrueORF Data for RC201539

Vector:	pCMV6-Entry Change vector?	Tag: C-terminal Myc-DDK	Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Sequence Data:	ORF Nucleotide Sequence CHROMATOGRAMS Protein Sequence	ORF Size: 351 bp Predicted Protein MW: 13.3 kDa
Restriction Sites:	SgfI-MluI Cloning Scheme for this gene Plasmid Map
OTI Annotation:	This clone was engineered to express the complete ORF with an expression tag.
OTI Disclaimer:	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components:	The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Protein Families:
Protein Pathways:	Oxidative phosphorylation Metabolic pathways Alzheimer's disease Parkinson's disease Huntington's disease

* The lysates used for this WB picture contain the overexpressed empty vector or the Myc-DDK tagged ORF clone.

Reference Data

RefSeq: NM_005000.2, NP_004991	RefSeq Size: 1550	RefSeq ORF: 351
Synonyms : B13; CI-13kB; CI-13KD-B; NUFM; UQOR13
LocusID: 4698	Cytogenetic: 7q31.33	Domains: ETC_CI_29_9
Summary: The human NDUFA5 gene codes for the B13 subunit of complex I of the respiratory chain, which transfers electrons from NADH to ubiquinone. The high degree of conservation of NDUFA5 extending to plants and fungi indicates its functional significance in the enzyme complex. The protein localizes to the inner mitochondrial membrane as part of the 7 component-containing, water soluble 'iron-sulfur protein' (IP) fraction of complex I, although its specific role is unknown. It is assumed to undergo post-translational removal of the initiator methionine and N-acetylation of the next amino acid. The predicted secondary structure is primarily alpha helix, but the carboxy-terminal half of the protein has high potential to adopt a coiled-coil form. The amino-terminal part contains a putative beta sheet rich in hydrophobic amino acids that may serve as mitochondrial import signal. Related pseudogenes have also been identified on four other chromosomes. [provided by RefSeq, Jul 2008].

ORF Clones Citations:

Intracellular Trafficking of the KV1.3 Potassium Channel Is Regulated by the Prodomain of a Matrix Metalloprotease, Hai M. Nguyen, Charles A. Galea, Galina Schmunk, Brian J. Smith, Robert A. Edwards, Raymond S. Norton, and K. George Chandy, J. Biol. Chem., Mar 2013; 288: 6451 - 6464. [KV1.3]

Renal Hypodysplasia Associates with a Wnt4 Variant that Causes Aberrant Canonical Wnt Signaling, Asaf Vivante, Michal Mark-Danieli, Miriam Davidovits, Orit Harari-Steinberg, Dorit Omer, Yehudit Gnatek, Roxana Cleper, Daniel Landau, Yael Kovalski, Irit Weissman, Israel Eisenstein, Michalle Soudack, Haike Reznik Wolf, Naomi Issler, Danny Lotan, Yair Anikster, and Benjamin Dekel, J. Am. Soc. Nephrol., Mar 2013; 24: 550 - 558. [WNT4]

Smad4-Irf6 genetic interaction and TGFß-mediated IRF6 signaling cascade are crucial for palatal fusion in mice, Jun-ichi Iwata, Akiko Suzuki, Richard C. Pelikan, Thach-Vu Ho, Pedro A. Sanchez-Lara, Mark Urata, Michael J. Dixon, and Yang Chai, Development, Mar 2013; 140: 1220 - 1230. [p21]

Mutation of the iron-sulfur cluster assembly gene IBA57 causes severe myopathy and encephalopathy, Nikhita Ajit Bolar, Arnaud Vincent Vanlander, Claudia Wilbrecht, Nathalie Van der Aa, Joél Smet, Boel De Paepe, Geert Vandeweyer, Frank Kooy, François Eyskens, Elien De Latter, Gwenda Delanghe, Paul Govaert, Jules Gerard Leroy, Bart Loeys, Roland Lill, Lut Van Laer, and Rudy Van Coster, Hum. Mol. Genet., Mar 2013; 10.1093/hmg/ddt107. [IBA57]

Mutations in Critical Domains Confer the Human mTOR Gene Strong Tumorigenicity, Avaniyapuram Kannan Murugan, Ali Alzahrani, and Mingzhao Xing, J. Biol. Chem., Mar 2013; 288: 6511 - 6521. [mTOR]

Specificity Profiling of Dual Specificity Phosphatase Vaccinia VH1-related (VHR) Reveals Two Distinct Substrate Binding Modes, Rinrada Luechapanichkul, Xianwen Chen, Hashem A. Taha, Shubham Vyas, Xiaoyan Guan, Michael A. Freitas, Christopher M. Hadad, and Dehua Pei, J. Biol. Chem., Mar 2013; 288: 6498 - 6510. [ROBO1]

Dissecting the Role of COPI Complexes in Influenza Virus Infection, Eileen Sun, Jiang He, and Xiaowei Zhuang, J. Virol., Mar 2013; 87: 2673 - 2685. [COPE]

ATF4 Protects Against Neuronal Death in Cellular Parkinson's Disease Models by Maintaining Levels of Parkin, Xiaotian Sun, Jin Liu, John F. Crary, Cristina Malagelada, David Sulzer, Lloyd A. Greene, and Oren A. Levy, J. Neurosci., Feb 2013; 33: 2398 - 2407. [Myc-tagged rat parkin]

Functional analysis of sucrase–isomaltase mutations from chronic lymphocytic leukemia patients, David Rodríguez, Andrew J. Ramsay, Víctor Quesada, Cecilia Garabaya, Elías Campo, José M. P. Freije, and Carlos López-Otín, Hum. Mol. Genet., Feb 2013; 10.1093/hmg/ddt078. [SI]

Mutation of the ATP-gated P2X2 receptor leads to progressive hearing loss and increased susceptibility to noise, Denise Yan, Yan Zhu, Tom Walsh, Dinghua Xie, Huijun Yuan, Asli Sirmaci, Taro Fujikawa, Ann Chi Yan Wong, Tze L. Loh, Lilin Du, M’hamed Grati, Srdjan M. Vlajkovic, Susan Blanton, Allen F. Ryan, Zheng-Yi Chen, Peter R. Thorne, Bechara Kachar, Mustafa Tekin, Hong-Bo Zhao, Gary D. Housley, Mary-Claire King, and Xue Z. Liu, PNAS, Feb 2013; 110: 2228 - 2233. [P2RX2 ]

More TrueClone Citations >>

NDUFA5 Mouse Clones

SKU	Description	Amount	Price	Shipping
MC207660	Mus musculus NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5 (Ndufa5), nuclear gene encoding mitochondrial protein as transfection-ready DNA	10 ug	$380	2 weeks
MR200501	Myc-DDK-tagged ORF clone of Mus musculus NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5 (Ndufa5), nuclear gene encoding mitochondrial protein as transfection-ready DNA	10 ug	$380	Next day
MG200501	GFP-tagged ORF clone of Mus musculus NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5 (Ndufa5) in a GFP expression vector	10 ug	$420	3 weeks

NDUFA5 Rat Clones

SKU	Description	Amount	Price	Shipping
RN211640	NativeORF clone of Rattus norvegicus NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 5 (Ndufa5), nuclear gene encoding mitochondrial protein as transfection-ready DNA	10 ug	$380	2 weeks
RR211640	Myc-DDK-tagged ORF clone of Rattus norvegicus NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 5 (Ndufa5), nuclear gene encoding mitochondrial protein as transfection-ready DNA	10 ug	$380	2 weeks

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NDUFA5 (NM_005000) Human cDNA ORF Clone

Western Blot Validated

ORF Clones Citations:

NDUFA5 Mouse Clones

NDUFA5 Rat Clones

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