UMPS (NM_000373) Human Tagged ORF Clone

CAT#: RC201324

UMPS (Myc-DDK-tagged)-Human uridine monophosphate synthetase (UMPS), transcript variant 1

Product Images

Circular map for RC201324

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  Western blot validation of overexpression lysate (Cat# LY424757) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201324 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified UMPS protein (Cat# TP301324). The protein was produced from HEK293T cells transfected with UMPS cDNA clone (Cat# RC201324) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: UMPS
• Synonyms: OPRT
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201324 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGTCGCTCGTGCAGCTTTGGGGCCATTGGTGACGGGTCTGTACGACGTGCAGGCTTTCAAGTTTG
  GGGACTTCGTGCTGAAGAGCGGGCTTTCCTCCCCCATCTACATCGATCTGCGGGGCATCGTGTCTCGACC
  GCGTCTTCTGAGTCAGGTTGCAGATATTTTATTCCAAACTGCCCAAAATGCAGGCATCAGTTTTGACACC
  GTGTGTGGAGTGCCTTATACAGCTTTGCCATTGGCTACAGTTATCTGTTCAACCAATCAAATTCCAATGC
  TTATTAGAAGGAAAGAAACAAAGGATTATGGAACTAAGCGTCTTGTAGAAGGAACTATTAATCCAGGAGA
  AACCTGTTTAATCATTGAAGATGTTGTCACCAGTGGATCTAGTGTTTTGGAAACTGTTGAGGTTCTTCAG
  AAGGAGGGCTTGAAGGTCACTGATGCCATAGTGCTGTTGGACAGAGAGCAGGGAGGCAAGGACAAGTTGC
  AGGCGCACGGGATCCGCCTCCACTCAGTGTGTACATTGTCCAAAATGCTGGAGATTCTCGAGCAGCAGAA
  AAAAGTTGATGCTGAGACAGTTGGGAGAGTGAAGAGGTTTATTCAGGAGAATGTCTTTGTGGCAGCGAAT
  CATAATGGTTCTCCCCTTTCTATAAAGGAAGCACCCAAAGAACTCAGCTTCGGTGCACGTGCAGAGCTGC
  CCAGGATCCACCCAGTTGCATCGAAGCTTCTCAGGCTTATGCAAAAGAAGGAGACCAATCTGTGTCTATC
  TGCTGATGTTTCACTGGCCAGAGAGCTGTTGCAGCTAGCAGATGCTTTAGGACCTAGTATCTGCATGCTG
  AAGACTCATGTAGATATTTTGAATGATTTTACTCTGGATGTGATGAAGGAGTTGATAACTCTGGCAAAAT
  GCCATGAGTTCTTGATATTTGAAGACCGGAAGTTTGCAGATATAGGAAACACAGTGAAAAAGCAGTATGA
  AGGAGGTATCTTTAAAATAGCTTCCTGGGCAGATCTAGTAAATGCTCACGTGGTGCCAGGCTCAGGAGTT
  GTGAAAGGCCTGCAAGAAGTGGGCCTGCCTTTGCATCGGGGGTGCCTCCTTATTGCGGAAATGAGCTCCA
  CCGGCTCCCTGGCCACTGGGGACTACACTAGAGCAGCGGTTAGAATGGCTGAGGAGCACTCTGAATTTGT
  TGTTGGTTTTATTTCTGGCTCCCGAGTAAGCATGAAACCAGAATTTCTTCACTTGACTCCAGGAGTTCAG
  TTGGAAGCAGGAGGAGATAATCTTGGCCAACAGTACAATAGCCCACAAGAAGTTATTGGCAAACGAGGTT
  CCGATATCATCATTGTAGGTCGTGGCATAATCTCAGCAGCTGATCGTCTGGAAGCAGCAGAGATGTACAG
  AAAAGCTGCTTGGGAAGCGTATTTGAGTAGACTTGGTGTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201324 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAVARAALGPLVTGLYDVQAFKFGDFVLKSGLSSPIYIDLRGIVSRPRLLSQVADILFQTAQNAGISFDT
  VCGVPYTALPLATVICSTNQIPMLIRRKETKDYGTKRLVEGTINPGETCLIIEDVVTSGSSVLETVEVLQ
  KEGLKVTDAIVLLDREQGGKDKLQAHGIRLHSVCTLSKMLEILEQQKKVDAETVGRVKRFIQENVFVAAN
  HNGSPLSIKEAPKELSFGARAELPRIHPVASKLLRLMQKKETNLCLSADVSLARELLQLADALGPSICML
  KTHVDILNDFTLDVMKELITLAKCHEFLIFEDRKFADIGNTVKKQYEGGIFKIASWADLVNAHVVPGSGV
  VKGLQEVGLPLHRGCLLIAEMSSTGSLATGDYTRAAVRMAEEHSEFVVGFISGSRVSMKPEFLHLTPGVQ
  LEAGGDNLGQQYNSPQEVIGKRGSDIIIVGRGIISAADRLEAAEMYRKAAWEAYLSRLGV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000373
• ORF Size: 1440 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000373.4
• RefSeq Size: 6738 bp
• RefSeq ORF: 1443 bp
• Locus ID: 7372
• UniProt ID: P11172
• Cytogenetics: 3q21.2
• Domains: Pribosyltran, OMPdecase
• Protein Families: Druggable Genome
• Protein Pathways: Drug metabolism - other enzymes, Metabolic pathways, Pyrimidine metabolism
• MW: 52.2 kDa
• Gene Summary: This gene encodes a uridine 5'-monophosphate synthase. The encoded protein is a bifunctional enzyme that catalyzes the final two steps of the de novo pyrimidine biosynthetic pathway. The first reaction is carried out by the N-terminal enzyme orotate phosphoribosyltransferase which converts orotic acid to orotidine-5'-monophosphate. The terminal reaction is carried out by the C-terminal enzyme OMP decarboxylase which converts orotidine-5'-monophosphate to uridine monophosphate. Defects in this gene are the cause of hereditary orotic aciduria. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]