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Home Antibody ELISA Kits

Zika Virus (ZIKV) Real Time RT-PCR Kit

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SKU Description Manual Price Availability*  
EA102173 Zika Virus (ZIKV) Real Time RT-PCR Kit Manual $399 In Stock Add to Shopping Cart

Specification


Product NameZika Virus (ZIKV) Real Time RT-PCR Kit
SizeRT-PCR kit
Gene ID
Gene Symbolzika
Assay TypeRT-PCR
Signalfluorescent
Format25 test
Assay Length6 hours
SpecificityThis kit is used for quantitative detection Zika virus in human serum
ReactivityHuman
Cross ReactivityThere is no detectable cross-reactivity with other relevant viruses.
InterferenceNo significant interference observed with available related molecules.
Sample Volume5 ul RNA extract/rxn
Sample TypeRNA extract
Sensitivity1×10^3copies/ml
Curve RangeLOQ: 2 x 10^3 to 1 x 10^8 copies/ml
BackgroundThe principle of the real-time detection is based on the fluorogenic 5’nuclease assay. During the PCR reaction, the DNA polymerase cleaves the probe at the 5’ end and separates the reporter dye from the quencher dye only when the probe hybridizes to the target DNA. This cleavage results in the fluorescent signal generated by the cleaved reporter dye, which is monitored real-time by the PCR detection system. The PCR cycle at which an increase in the fluorescence signal is detected initially is proportional to the amount of the specific PCR product. Monitoring the fluorescence intensities in real-time allows the detection of the accumulating product without having to re-open the reaction tube after the amplification.
Components
  • 1. ZIKV Super Mix, 1 vial, 480 µl
  • 2. RT-PCR Enzyme Mix, 1 vial, 28 µl
  • 3. Molecular Grade Water, 1 vial, 400 µl
  • 4. Internal Control (IC), 1 vial, 30 µl
  • 5. ZIKV Positive Control (1×10^7copies/ml), 1 vial, 30 µl
Standard Curve spacer

The standard curve may vary based on the QC data of each batch. Please refer to the actual datasheet along with shipment.

 

 

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