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Home Antibody ELISA Kits

Mouse CXCL16 ELISA Kit

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SKU Description Manual Price Availability*  
EA100472 Mouse CXCL16 ELISA Kit Manual $400 2-3 Weeks Add to Shopping Cart

Specification


Product NameMouse CXCL16 ELISA Kit
Size1x96 well plate
Gene ID66102
Gene SymbolCxcl16
Assay TypeSandwich ELISA kit of Quantitative Detection for Mouse CXCL16
SignalColorimetric
Format8×12 divisible strips
Assay Length3.5 hours incubations; 1 hour washing and analyzing samples
SpecificityThis kit is used for quantitative detection of mouse CXCL16
ReactivityMouse
Cross ReactivityThere is no detectable cross-reactivity with other relevant proteins.
InterferenceNo significant interference observed with available related molecules.
Sample Volume100 ul
Sample TypeCell culture supernates, serum and plasma(heparin, EDTA).
Sensitivity<1pg/ml
Curve Range15.6pg/ml-1000pg/ml
BackgroundChemokine(C-X-C motif) ligand 16(CXCL16) is a small cytokine belonging to the CXC chemokine family. Larger than other chemokines(with 254 amino acids), CXCL16 is composed of a CXC chemokine domain, a mucin-like stalk, a transmembrane domain and a cytoplasmic tail containing a potential tyrosine phosphorylation site that may bind SH2. These are unusual features for a chemokine, and allow CXCL16 to be expressed as a cell surface bound molecule, as well as a soluble chemokine. CXCL16 is produced by dendritic cells found in the T cell zones of lymphoid organs, and by cells found in the red pulp of the spleen. Cells that bind and migrate in response to CXCL16 include several subsets of T cells, and natural killer T(NKT) cells. CXCL16 interacts with the chemokine receptor CXCR6, also known as Bonzo. Expression of CXCL16 is induced by the inflammatory cytokines IFN-gamma and TNF-alpha.2 The gene for human CXCL16 is located on chromosome 17p13. The standard product used in this kit is recombinant mouse CXCL16, consisting of 88 amino acids with the molecular mass of 9.9KDa.
Components
  • 96-well plate precoated with anti- mouse CXCL16 antibody 1
  • Lyophilized recombinant mouse CXCL16 standard 10ng/tubex2
  • Biotinylated anti- mouse CXCL16 antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
Standard Curve spacer

The standard curve may vary based on the QC data of each batch. Please refer to the actual datasheet along with shipment.

 

 

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