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Home Antibody ELISA Kits

Human CXCL16 ELISA Kit

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SKU Description Manual Price Availability*  
EA100471 Human CXCL16 ELISA Kit Manual $389 2-3 Weeks Add to Shopping Cart

Specification


Product NameHuman CXCL16 ELISA Kit
Size1x96 well plate
Gene ID58191
Gene SymbolCXCL16
Assay TypeSandwich ELISA kit of Quantitative Detection for Human CXCL16
SignalColorimetric
Format8×12 divisible strips
Assay Length3.5 hours incubations; 1 hour washing and analyzing samples
SpecificityThis kit is used for quantitative detection of human CXCL16
ReactivityHuman
Cross ReactivityThere is no detectable cross-reactivity with other relevant proteins.
InterferenceNo significant interference observed with available related molecules.
Sample Volume100 ul
Sample TypeCell culture supernates, serum and plasma(heparin, EDTA).
Sensitivity<10pg/ml
Curve Range93.7pg/ml-6000pg/ml
BackgroundChemokine(C-X-C motif) ligand 16(CXCL16) is a small cytokine belonging to the CXC chemokine family. Larger than other chemokines(with 254 amino acids), CXCL16 is composed of a CXC chemokine domain, a mucin-like stalk, a transmembrane domain and a cytoplasmic tail containing a potential tyrosine phosphorylation site that may bind SH2. These are unusual features for a chemokine, and allow CXCL16 to be expressed as a cell surface bound molecule, as well as a soluble chemokine. CXCL16 is produced by dendritic cells found in the T cell zones of lymphoid organs, and by cells found in the red pulp of the spleen. Cells that bind and migrate in response to CXCL16 include several subsets of T cells, and natural killer T(NKT) cells. CXCL16 interacts with the chemokine receptorCXCR6, also known as Bonzo. The novel CXC-chemokine ligand 16(CXCL16) functions as transmembrane adhesion molecule on the surface of APCs and as a soluble chemoattractant for activated T cells. Expression of CXCL16 is induced by the inflammatory cytokines IFN-gamma and TNF-alpha. The gene for human CXCL16 is located on chromosome 17.
Components
  • 96-well plate precoated with anti- human CXCL16 antibody 1
  • Lyophilized recombinant human CXCL16 standard 10ng/tubex2
  • Biotinylated anti- human CXCL16 antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
Standard Curve spacer

The standard curve may vary based on the QC data of each batch. Please refer to the actual datasheet along with shipment.

 

 

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