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Home Antibody ELISA Kits

Human sL-Selectin ELISA Kit

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SKU Description Manual Price Availability*  
EA100348 Human sL-Selectin ELISA Kit Manual $389 2-3 Weeks Add to Shopping Cart

Specification


Product NameHuman sL-Selectin ELISA Kit
Size1x96 well plate
Gene ID6402
Gene SymbolSELL
Assay TypeSandwich ELISA kit of Quantitative Detection for Human sL-Selectin
SignalColorimetric
Format8×12 divisible strips
Assay Length3.5 hours incubations; 1 hour washing and analyzing samples
SpecificityThis kit is used for quantitative detection of human L-Selectin
ReactivityHuman
Cross ReactivityThere is no detectable cross-reactivity with other relevant proteins.
InterferenceNo significant interference observed with available related molecules.
Sample Volume100 ul
Sample TypeCell culture supernates, serum and plasma(heparin, EDTA, citrate).
Sensitivity<5pg/ml
Curve Range62.5pg/ml-4000pg/ml
BackgroundL-selectin, also known as CD62L, is a cell adhesion molecule found on leukocytes. It belongs to the selectin family of proteins, which recognize sialylated carbohydrate groups. It is cleaved by ADAM17.SELL(L-selectin)is a cell surface component that is a member of a family of adhesion/homing receptors which play important roles in leukocyte-endothelial cell interactions. The molecule is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4 binding proteins.1L-selectin acts as a "homing receptor" for leukocytes to enter secondary lymphoid tissues via high endothelial venules. Ligands present on endothelial cells will bind to leukocyte expressing L-selectin, slowing leukocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point2.
Components
  • 96-well plate precoated with anti- human sL-Selectin antibody 1
  • Lyophilized recombinant human sL-Selectin standard 10ng/tubex2
  • Biotinylated anti- human sL-Selectin antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
Standard Curve

Representative standard curve for Human sL-Selectin PicoKine ELISA Kit

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The standard curve may vary based on the QC data of each batch. Please refer to the actual datasheet along with shipment.

 

 

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