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Home Antibody ELISA Kits

Mouse MDC ELISA Kit

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SKU Description Manual Price Availability*  
EA100308 Mouse MDC ELISA Kit Manual $389 2-3 Weeks Add to Shopping Cart

Specification


Product NameMouse MDC ELISA Kit
Size1x96 well plate
Gene ID20299
Gene SymbolCcl22
Assay TypeSandwich ELISA kit of Quantitative Detection for Mouse MDC
SignalColorimetric
Format8×12 divisible strips
Assay Length3.5 hours incubations; 1 hour washing and analyzing samples
SpecificityThis kit is used for quantitative detection of mouse MDC
ReactivityMouse
Cross ReactivityThere is no detectable cross-reactivity with other relevant proteins.
InterferenceNo significant interference observed with available related molecules.
Sample Volume100 ul
Sample TypeCell culture supernates, serum and plasma(heparin, EDTA).
Sensitivity<1pg/ml
Curve Range15.6pg/ml-1000pg/ml
BackgroundMacrophage-derived chemokine(MDC), also called Chemokine, cc motif, ligand 22(CCL22) or Small inducible cytokine subfamily A, member 22(SCY22). MDC is a recently identified member of the CC chemokine family. It is not closely related to other chemokines, sharing most similarity with thymus- and activation-regulated chemokine(TARC), which contains 37% identical amino acids. In addition, MDC gene is mapped to chromosome 16q13, the same position reported for the TARC gene. MDC has the four-cysteine motif and other highly conserved residues characteristic of CC chemokines, but it shares<35% identity with any of the known chemokines. Recombinant MDC was expressed in Chinese hamster ovary cells and purified by heparin-Sepharose chromatography. MDC is highly expressed in macrophages and in monocyte-derived dendritic cells, but not in monocytes, natural killer cells, or several cell lines of epithelial, endothelial, or fibroblast origin. High expression was also detected in normal thymus and less expression in lung and spleen. MDC is thus a unique member of the CC chemokine family that may play a fundamental role in the function of dendritic cells, natural killer cells, and monocytes.
Components
  • 96-well plate precoated with anti- mouse MDC antibody 1
  • Lyophilized recombinant mouse MDC standard 10ng/tubex2
  • Biotinylated anti- mouse MDC antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30 ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
Standard Curve

Representative standard curve for Mouse MDC PicoKine ELISA Kit

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The standard curve may vary based on the QC data of each batch. Please refer to the actual datasheet along with shipment.

 

 

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