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Home CRISPR-CAS9 KN320024

Zfp91 - mouse gene knockout kit via CRISPR

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Specifications  Citations  Validation Data  FAQ
SKU Description Price Availability Manual  
KN320024 Zfp91 - mouse gene knockout kit via CRISPR $1200 4 weeks Manual PDF Add to Shopping Cart
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Also for Zfp91 (Locus ID 109910)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Request Antibody
SKU Description Donor Vector Price Availability  
KN320024RB Zfp91 - mouse gene knockout kit via CRISPR RFP-BSD 1290 4 weeks
KN320024LP Zfp91 - mouse gene knockout kit via CRISPR Luciferase-Puro 1290 4 Weeks
KN320024BN Zfp91 - mouse gene knockout kit via CRISPR mBFP-Neo 1290 4 Weeks
Add to Shopping Cart

Comparing to KN320024, the above kits contain:

  • Identical gRNA vectors
  • Identical LHA & RHA
  • Different donor cassette
kn_donor_diagram
Kit Components
KN320024G1, Zfp91 gRNA vector 1 in pCas-Guide vector
KN320024G2, Zfp91 gRNA vector 2 in pCas-Guide vector
KN320024D, donor vector containing Left and right homologous arms and GFP-Puro functional cassette.
   
GE100003, scramble sequence in pCas-Guide vector

Disclaimer
The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data
RefSeq: NM_001039718NM_053009
Synonyms: 9130014I08Rik; A530054C17Rik; AL024263; AW545902; Pzf; Zfp-91
Summary: The protein encoded by this gene is a member of the zinc finger family of proteins. The gene product contains C2H2-type domains, which are the classical zinc finger domains found in numerous nucleic acid-binding proteins. The homologous human protein has been shown to function as a regulator of the non-canonical NF-kappaB pathway in lymphotoxin-beta receptor signaling. A read-through transcript variant composed of Zfp91 and the downstream Cntf gene sequence has been identified, but it is thought to be non-coding. Read-through transcription of Zfp91 and Cntf has been observed in both human and mouse. A Zfp91-related pseudogene has also been identified on chromosome 17. [provided by RefSeq, Oct 2010].

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Diagram-Gene Editing RecombII


 

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