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Home CRISPR-CAS9 KN303124

Cenpa - mouse gene knockout kit via CRISPR

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Specifications  Citations  Validation Data  FAQ
SKU Description Price Availability Manual  
KN303124 Cenpa - mouse gene knockout kit via CRISPR $1200 4 weeks Manual PDF Add to Shopping Cart
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Also for Cenpa (Locus ID 12615)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Request Antibody
SKU Description Donor Vector Price Availability  
KN303124RB Cenpa - mouse gene knockout kit via CRISPR RFP-BSD 1290 4 weeks
KN303124LP Cenpa - mouse gene knockout kit via CRISPR Luciferase-Puro 1290 4 Weeks
KN303124BN Cenpa - mouse gene knockout kit via CRISPR mBFP-Neo 1290 4 Weeks
Add to Shopping Cart

Comparing to KN303124, the above kits contain:

  • Identical gRNA vectors
  • Identical LHA & RHA
  • Different donor cassette
kn_donor_diagram
Kit Components
KN303124G1, Cenpa gRNA vector 1 in pCas-Guide vector
KN303124G2, Cenpa gRNA vector 2 in pCas-Guide vector
KN303124D, donor vector containing Left and right homologous arms and GFP-Puro functional cassette.
   
GE100003, scramble sequence in pCas-Guide vector

Disclaimer
The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data
RefSeq: BC011038NM_001302129NM_001302130NM_001302131NM_001302132NM_007681NR_126074
Synonyms: Cenp-A
Summary: Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. This gene encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. Centromere protein A is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle. The protein is a replication-independent histone that is a member of the histone H3 family. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Nov 2015].

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Diagram-Gene Editing RecombII


 

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