FAK (PTK2) Human Gene Knockout Kit (CRISPR)

CAT#: KN219839

PTK2 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: FAK
• Locus ID: 5747
• Components:

  KN219839G1, FAK gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: CAGGGCAATTGCTTGAACCT

  KN219839G2, FAK gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: GGAGTGGAGTGGCATGATCT

  KN219839D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  AGGGCCACTT TTCATCGCCA CCTGGGGTAG CTTGCCAGCA CATGTTTACC TTTGGTTTGG GATCACAGCA AGCAGAAGAG ATGAAAGCTC CCTTTAACCG TTCTAGGATA TGTATTTGAA TTGGAGAAAC TACCTCAAAA TAGAGTGTGT GCAGGCACTA TATGAAGGCT TCTCAGCGTT TAAGTGCATA CATGCCACCC AGGGATCTCG TTATATTTTA AGTTCCAATT CAGTCTGTCT GGGAAGGGCT CAGACTTGCA TTTTTCCTAA GTTCCCAGGT GATGCCAGGG CTGGTGGTCT GTGGACCATA CTTGGAGTAG CTAGGCATTG GTTTTCAAAG TGTGGGTTGC AAGGTGGCAC TGAGTCTTAG TTTGAAAGTT GAATAACCAC TGTTGATTGC GGTAGTTAAG GGCATGGAGT TGGGGTCAGA ATCCTGGCTC TATGATCTTG GGCTTGTTTG TTAAGTTTTC TAATCTCTAT TTCCTTGTGT GGCATGTGAT TGTACTACAT GATTATGTAA TGCTTAGTCT TTTTTTTTTG TTTTTTTTTG AGACAGAGTC TTGCTGTGTC ACCCAGGCTG CCAGGCTGGA GTGGAGTGGC CTCTCTCTAG GACAGTGATG AACCAGCCTT CTTGTACTGG AGGAATACAC CATCCGTCTT CATAAAGACT GGCAGGCACA TGGACAAGTT TACCAGTTAT AATGCTTTTC TTACACATTA AAGAAGTAGG TGTTTGTATT GGGGCTTCAA CAAGGACTTG GAAAACTTTA AGATAATACA ACTACATAGT ATTCCTTTAG GTGAATATCG TTTCAGTTTC TCAGATCATT GATGAAAGCC TTAACAAAGA TTTTTTTTTT TTTTTTTTTT TTGAGATGGA GTCTTACTCT GTTGCCCAGG CTGAAGTGCA TGATCTTGGC TCACTGCAAC CTTCACCTCC TGGGTTCAAG CAATTCTCCT GCCTCAGCCT TCCGAGTAGC TGGGATTACA GGCACCTGTC ACCATGTGTG GCTAATTTTT GCATTTTTAG TAGAGACAGG GTTTTGCTCT GTTGGCCAGG CTGGTCTCAA ACTCCTGACC TCAGAAGATC CGCCTGCCTT GGCCTCCCAA AGTGCTGGGA TTGCAGGTGT GAGCCACCAC ACCCGGCCTA GATTTTAAAA TAAGCATCTG TTTGTTTCTT TTTTTAGTTG CCACTAGTAA

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001199649, NM_001316342, NM_005607, NM_153831, NM_001352694, NM_001352695, NM_001352696, NM_001352697, NM_001352698, NM_001352699, NM_001352700, NM_001352701, NM_001352702, NM_001352703, NM_001352704, NM_001352705, NM_001352706, NM_001352707, NM_001352708, NM_001352709, NM_001352710, NM_001352711, NM_001352712, NM_001352713, NM_001352714, NM_001352715, NM_001352716, NM_001352717, NM_001352718, NM_001352719, NM_001352720, NM_001352721, NM_001352722, NM_001352723, NM_001352724, NM_001352725, NM_001352726, NM_001352727, NM_001352728, NM_001352729, NM_001352730, NM_001352731, NM_001352732, NM_001352733, NM_001352734, NM_001352735, NM_001352736, NM_001352737, NM_001352738, NM_001352739, NM_001352740, NM_001352741, NM_001352742, NM_001352743, NM_001352744, NM_001352745, NM_001352746, NM_001352747, NM_001352748, NM_001352749, NM_001352750, NM_001352751, NM_001352752, NR_148036, NR_148037, NR_148038, NR_148039
• UniProt ID: Q05397
• Synonyms: FADK; FAK; FAK1; FRNK; p125FAK; pp125FAK; PPP1R71
• Summary: This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2017]