PI 3 Kinase catalytic subunit alpha (PIK3CA) Human Gene Knockout Kit (CRISPR)

CAT#: KN213112

PIK3CA - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: PI 3 Kinase catalytic subunit alpha
• Locus ID: 5290
• Components:

  KN213112G1, PI 3 Kinase catalytic subunit alpha gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: AGTTCACCTGATGATGGTCG

  KN213112G2, PI 3 Kinase catalytic subunit alpha gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: GGATTCTTGGGGGCATCAAG

  KN213112D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  CCATAAAATT TCTCATTATT ACAGGTTGAG GGCAGGGAGT GAAAAAGGAA AAGGATTTGA CTTCTCTAGG CATGGTTTTA CTTATTTTAA AATAAGAGCA GTGGACCCAT GATCTGAAAG ATTGTTTCTG GCCTAAATTC TGTTTTTCCG TAAGATAGAA GAGAATGATA AAGGTGATAT GCAATGGGAA TTTTGGGTTG AATTGCAACT TACTTTTCAT TCTTCCCATA GCCTCATCTC TGTCTTGATG AAACCTTAGA GAATCCACTC AAATATAGTG TTTTCTTAAA TGACTTTATT AAATTTAATT AAATACAAAT GGTTTTTCTA ATCTCTGCTG AAATAAATTC TTTGTAGCCT AATTTAGAGC AACAGTCTAG ATTAGAATAC TTAAGATGAA TTATTACTAC TTAGCCTAAT CAAGTCAAAC TATGGAAAAT GAGTTTTTAC ATTTTAGCAG TGTTATAAAT GTATTCTTCT GTAGTTGTGT CTCTTTTAAC ATATGTTTTC CTTCTTTGAT TTAGGTTTCT GCTTTGGGAC AACCATACAT CTAATTCCTT AAAGTAGTTT TATATGTAAA ACTTGCAAAG AATCAGAACA TAGTAGAATG TTTACTACCA AATGGAATGA TAGTGACTTT AGAATGCCTC CGTGAGGCTA CATTAATAAC CATAAAGCAT GAACTATTTA AAGAAGCAAG AAAATACCCC CTCCATCAAC TTCTTCAAGA TGAATCTTCT TACATTTTCG TAAGTGTTAC TCAAGAAGCA GAAAGGGAAG AATTTTTTGA TGAAACAAGA CGACTTTGTG ACCTTCGGCT TTTTCAACCC TTTTTAAAAG TAATTGAACC AGTAGGCAAC CGTGAAGAAA AGATCCTCAA TCGAGAAATT GGTATGATAC AATATCCTAT TCTAAAATGC AAATAACCAT AAAGCTTAAC TGTTGTCCCT TTCTAAAATA TTTCTGTCTA AACCAATACC TTCGTAATCT TAAATAGCTT TCTAAATAAA AATCATAAAT CTAAAGTATG TTTTACTATC GAACTATGGA ACTATTTTTA ACACCTTGAT ATTATTCCAT AAGGTTTTAT TTAAGAAATG TCATTTGTGG GATGACTTAG ATTTGTTATA TCTCAGTGTT GTTATTCTTT TAAAAATGAT TGATAGGAAT GTTTGCTGCC TTTGCTCTAA ATTGCTGAAT

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_006218
• UniProt ID: P42336
• Synonyms: CLOVE; CWS5; MCAP; MCM; MCMTC; p110-alpha; PI3K; PI3K-alpha
• Summary: Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers. A pseudogene of this gene has been defined on chromosome 22. [provided by RefSeq, Apr 2016]