HDAC1 Human Gene Knockout Kit (CRISPR)

CAT#: KN201745

HDAC1 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: HDAC1
• Locus ID: 3065
• Components:

  KN201745G1, HDAC1 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GGTGAGCACCGTCCTCGCGG

  KN201745G2, HDAC1 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: TGAGCACCGTCCTCGCGGCG

  KN201745D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGTTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTTC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCTATG ACCATGATTA CGCCAAGCTC CTTCCTCTTC CAGCCCTTCC TCTTTCACTG ACTTACTGAC TGGAAGACAC ACTCACAGCA GCCCCTCGAC AAACATTAGC TTTCTTTCCC TTTCTTGTCG GTTTCTTCCT CTCCAAACCC GCGTGTTGCT TTTCTTTTTA ATTATTTTTC TGTAGCCCTC CTTTGCGGCC ACAAACTCGC TTTCTAACCC AGGTTCAGCC CTTTTATTGG CTGAGTGACC TTGTGCAAGT CACTTTTCCC CTGTAGGCCT CGGTTTATTC TCCGTAAAAT CAGAAAGTTG GCCTCCGATC TCCAAGCACG CTTTTCACGA CGAAGTGGGA CTGTTAAGTT TACAGAGCTG CTTTCCTCCC CCGGGACTGA TGGTACGGTC CCCGGGCGGC TCCCCACCCA TCTGTCGCAG ACCTTGGTAC AGGCCCAGGG GGCCCTCGGC GGCCTCTCCG GGCTGCCCTT GCCCCCTGCC GAGTTCCGGG CCCGTGTCTG CGCAAGCTGA TTGGCTGGAG CGGTGCCCGG GCTGCGCGGC TATAGGTGAG CCCAGGAGGG GACGGGCGGG GCGGGCCGGA GGCCCGCCCC CTCCCCCCTG GGTCGGACGC TGAGCGGAGC CGCGGGCGGG AGGGCGGACG GACCGACTGA CGGTAGGGAC GGGAGGCGAG CAAGACTAGC ATGGAGAGCG ACGAGAGCGG CCTGCCCGCC ATGGAGATCG AGTGCCGCAT CACCGGCACC CTGAACGGCG TGGAGTTCGA GCTGGTGGGC GGCGGAGAGG GCACCCCCGA GCAGGGCCGC ATGACCAACA AGATGAAGAG CACCAAAGGC GCCCTGACCT TCAGCCCCTA CCTGCTGAGC CACGTGATGG GCTACGGCTT CTACCACTTC GGCACCTACC CCAGCGGCTA CGAGAACCCC TTCCTGCACG CCATCAACAA CGGCGGCTAC ACCAACACCC GCATCGAGAA GTACGAGGAC GGCGGCGTGC TGCACGTGAG CTTCAGCTAC CGCTACGAGG CCGGCCGCGT GATCGGCGAC TTCAAGGTGA TGGGCACCGG CTTCCCCGAG GACAGCGTGA TCTTCACCGA CAAGATCATC CGCAGCAACG CCACCGTGGA GCACCTGCAC CCCATGGGCG ATAACGATCT GGATGGCAGC TTCACCCGCA CCTTCAGCCT GCGCGACGGC GGCTACTACA GCTCCGTGGT GGACAGCCAC ATGCACTTCA AGAGCGCCAT CCACCCCAGC ATCCTGCAGA ACGGGGGCCC CATGTTCGCC TTCCGCCGCG TGGAGGAGGA TCACAGCAAC ACCGAGCTGG GCATCGTGGA GTACCAGCAC GCCTTCAAGA CCCCGGATGC AGATGCCGGT GAAGAAAGAG TTTAAGAATT CCGATCATAT TCAATAACCC TTAATATAAC TTCGTATAAT GTATGCTATA CGAAGTTATT AGGTCTGAAG AGGAGTTTAC GTCCAGCCAA GCTTAGGATC TCGACCTCGA AATTCTACCG GGTAGGGGAG GCGCTTTTCC CAAGGCAGTC TGGAGCATGC GCTTTAGCAG CCCCGCTGGG CACTTGGCGC TACACAAGTG GCCTCTGGCC TCGCACACAT TCCACATCCA CCGGTAGGCG CCAACCGACT CCGTTCTTTG GTGGCCCCTT CGCGCCACCT TCTACTCCTC CCCTAGTCAG GAAGTTCCCC CCCGCCCCGC AGCTCGCGTC GTGCAGGACG TGACAAATGG AAGTAGCACG TCTCACTAGT CTCGTGCAGA TGGACAGCAC CGCTGAGCAA TGGAAGCGGG TAGGCCTTTG GGGCAGCGGC CAATAGCAGC TTTGCTCCTT CGCTTTCTGG GCTCAGAGGC TGGGAAGGGG TGGGTCCGGG GGCGGGCTCA GGGGCGGGCT CAGGGGCGGG GCGGGCGCCC GAAGGTCCTC CGGAGGCCCG GCATTCTGCA CGCTTCAAAA GCGCACGTCT GCCGCGCTGT TCTCCTCTTC CTCATCTCCG GGCCTTTCGA CCTGCATCCA TCTAGATCTC GAGCAGCTGA AGCTTACCAT GACCGAGTAC AAGCCCACGG TGCGCCTCGC CACCCGCGAC GACGTCCCCA GGGCCGTACG CACCCTCGCC GCCGCGTTCG CCGACTACCC CGCCACGCGC CACACCGTCG ATCCGGACCG CCACATCGAG CGGGTCACCG AGCTGCAAGA ACTCTTCCTC ACGCGCGTCG GGCTCGACAT CGGCAAGGTG TGGGTCGCGG ACGACGGCGC CGCGGTGGCG GTCTGGACCA CGCCGGAGAG CGTCGAAGCG GGGGCGGTGT TCGCCGAGAT CGGCCCGCGC ATGGCCGAGT TGAGCGGTTC CCGGCTGGCC GCGCAGCAAC AGATGGAAGG CCTCCTGGCG CCGCACCGGC CCAAGGAGCC CGCGTGGTTC CTGGCCACCG TCGGCGTCTC GCCCGACCAC CAGGGCAAGG GTCTGGGCAG CGCCGTCGTG CTCCCCGGAG TGGAGGCGGC CGAGCGCGCC GGGGTGCCCG CCTTCCTGGA GACCTCCGCG CCCCACAACC TCCCCTTCTA CGAGCGGCTC GGCTTCACCG TCACCGCCGA CGTCGAGGTG CCCGAAGGAC CGCGCACCTG GTGCATGACC CGCAAGCCCG GTGCCTGACG CCCGCCCCAC GACCCGCAGC GCCCGACCGA AAGGAGCGCA CGACCCCATG CATCGATGAT ATCAGATCCC CGGGATGCAG AAATTGATGA TCTATTAAAC AATAAAGATG TCCACTAAAA TGGAAGTTTT TCCTGTCATA CTTTGTTAAG AAGGGTGAGA ACAGAGTACC TACATTTTGA ATGGAAGGAT TGGAGCTACG GGGGTGGGGG TGGGGTGGGA TTAGATAAAT GCCTGCTCTT TACTGAAGGC TCTTTACTAT TGCTTTATGA TAATGTTTCA TAGTTGGATA TCATAATTTA AACAAGCAAA ACCAAATTAA GGGCCAGCTC ATTCCTCCCA CTCATGATCT ATAGATCTAT AGATCTCTCG TGGGATCATT GTTTTTCTCT TGATTCCCAC TTTGTGGTTC TAAGTACTGT GGTTTCCAAA TGTGTCAGTT TCATAGCCTG AAGAACGAGA TCAGCAGCCT CTGTTCCACA TACACTTCAT TCTCAGTATT GTTTTGCCAA GTTCTAATTC CATCAGAAGC TGGTCGAGAT CCGGAACCCT TAATATAACT TCGTATAATG TATGCTATAC GAAGTTATTA GGTCCCTCGA AGAGGTTCAC TAGGCGCGCC GCTGAGGCGC TGGGGAGAGG CTCGGAGAGG AGGCTGCGAG GGGGAAGTCG AGGCTGAGGG AGGAGGCTGC GAGGAAGGGA GATCGAGGCT GAGACCAAGA GGGGATCGCG TGGGGGAAGC GTGAGGTAAT CTTGATGGGA AGATTCTGAG GAAGTCTGAT GTGGAGAGTT TGAGTGGGAC TCCTAGAGGG GAGGGTGCGT CGGAGCGGGG AGGCTGAATC TGATGGAGAC GGCTGCAAGG ATACATTTTC TTGCCGAGAA CCCAAAGCTC TTCCCTGCCC CGTCCCTTCC TCATCTTTTC TGGGGGAAGA GTCCTGCACG ACTGGTTGCC CGCCTCCCCT CCCCCAGCCT CTGAGCGGGG CCTGCGGTTG CTACTGCGAC GTGGGGCGCC AGGGGTTGCA AGGAGAAGGA TGCAACCTGT TAAAACAGAC TTCTCACTTA ATCTCTCAAG CAGGTTACTG TGTTCGAGGA AGTGTTGAGG GGCTGGGGCC GTGGGGTTGA TCGGGTCACC CTTCCCAGTG ACCGAAGTTC TCTCCGTGTC AGAGCTCAGA GTGTTAGGGG AGCACAGAGA AGAGGCACCG AACTCCACCT GAGGAGTCAG AGTGCAGTCT TCACTGACTG ACTGACTGGA AAGTCCTCTC CACTGACTGT AGCCTCCAAT TCACTGGCCG TCGTTTTACA ACGTCGTGAC TGGGAAAACC CTGGCGTTAC CCAACTTAAT CGCCTTGCAG CACATCCCCC TTTCGCCAGC TGGCGTAATA GCGAAGAGGC CCGCACCGAT CGCCCTTCCC AACAGTTGCG CAGCCTGAAT GGCGAATGGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATACGTCAAA GCAACCATAG TACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTTGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGGCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTTAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CAACGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGACCCGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTT

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_004964
• UniProt ID: Q13547
• Synonyms: GON-10; HD1; RPD3; RPD3L1
• Summary: Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. [provided by RefSeq, Jul 2008]