Home CRISPR-CAS9 GE100013
||pT7-Guide vector for target sequence cloning; IVT to produce gRNA
- T7 driven gRNA cloning vector
- gRNA can be produced via in vitro transcription after the target sequence being cloned; BamH I and BsmB I are cloning sites.
- For mRNA mediated genome editing via microinjection or mRNA transfection, both Cas9 mRNA and gRNA transcript are needed. T7 driven Cas9 mRNA can be produced from a separate vector, SKU GE100014.
- M13 forward sequencing primer can be used to sequence the target sequence after cloning.
- Download vector sequence