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Home cDNA Clone CRISPR/Cas9

CRISPR/Cas9 Genome Editing

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Overview

The latest tool in genome editing – CRISPR/Cas9 – allows for specific

genome disruption and replacement in a flexible and simple system resulting in high specificity and low cell toxicity. The CRISPR/Cas9 genome editing system requires the co- expression of a Cas9 protein with a guide RNA vector expressed from the human U6 polymerase III promoter. With the protospacer-adjacent motif (PAM - the sequence NGG) present at the 3′ end, Cas9 will unwind the DNA duplex and cleave both strands upon recognition of a target sequence by the guide RNA.

Then using a functional cassette synthesized by Blue Heron the rescue donor vector can then be inserted into the unwound DNA. The repaired genome will now express your desired sequence with or without tags.

Together, OriGene and Blue Heron are your complete CRISPR/Cas9 solution.

Validation data for pCas-Guide system PDF


CRISPR/Cas9-genome editing products

Cat # Product Name Description Price Availability Manual  
GE100001 pCas-Guide Cloning Kit Precut pCAS-Guide cloning kit (10 RXNs), GE100007 and GE100008 included. $395 In Stock Manual Add To Cart
GE100002 pCas-Guide pCAS-Guide vector (with Cas9 expression) for genomic target sequence cloning (10 µg), GE100008 included. $450 In Stock Manual Add To Cart
GE100011 pCas-Guide-GFP Cloning Kit Precut pCas-Guide-GFP cloning kit (10 RXNs), GE100007 and GE100008 included. $395 2 weeks Manual Add To Cart
GE100012 pCas-Guide-GFP pCas-Guide-GFP vector (with Cas9 and GFP expression) for genomic target sequence cloning (10 µg), GE100008 included. $450 2 weeks Manual Add To Cart
GE100013 pT7-Guide pT7-Guide vector for target sequence cloning; IVT to produce gRNA $320 2 weeks Manual Add To Cart
GE100014 pT7-Cas9 pT7-Cas9 vector in which Cas9 is under T7 for IVT to produce Cas9 mRNA $320 2 weeks Manual Add To Cart
GE100009 pLenti-Cas-Guide Cloning Kit Precut pLenti-Cas-Guide cloning kit (10 RXNs), GE100007 and GE100008 included. $395 2 Weeks Manual Add To Cart
GE100010 pLenti-Cas-Guide pLenti-Cas-Guide vector (with Cas9 expression) for genomic target sequence cloning (10 µg), GE100008 included. $450 2 Weeks Manual Add To Cart
GE100003 pCas-Scramble pCAS-Scramble, pCas-Guide vector with a scrambled sequence as a negative control (10 µg) $350 In Stock Manual Add To Cart
GE100007 Annealing Buffer Oligo annealing buffer (10X) $70 In Stock Manual Add To Cart
GE100008 CF3 Primer CF3, forward sequencing primer to sequence the targeting gRNA cloned into pCas9-Guide vector $50 In Stock Manual Add To Cart

Free gRNA designing tool  gRNA design

CRISPR/Cas9 Custom Services

Custom ServicesReference Price
Target sequence cloning into CRISPR/Cas9 vectors$345
$150
Donor vector construction with a predesigned cassette~$800
1kb of homologous arms (left + right)
Donor vector of your own designGene synthesis cost, $0.4-0.5/bp

Submit Request

The premade functional cassettes:

  • GFP-LoxP-PGK-Puro-LoxP
  • RFP-LoxP-PGK-BSD-LoxP
  • Luc-LoxP-PGK-Puro-LoxP

Commonly used genome editing experimental design

Diagram-Gene Editing RecombII

References

 

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