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Home CRISPR/Cas9

CRISPR/Cas9 Genome Editing

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Genome-editing
Knockout Kit
CRISPR/Cas9
Vectors
gRNA Cloning and
Donor Construction
AAVS1
Transgene Insertion
CRISPR/Cas
Starter Kit
Click to view the details of CRISPR/CAS9

CAS9 Antibody
Features

  • Complete kit for gene knockout via CRISPR (targeted sites around the 5’ end of the ORF)
  • 2 guide RNA vectors in pCas-Guide to ensure an efficient cleavage
  • Donor vector with predesigned homologous arms
  • Knockin GFP-Puro for selection
  • pCas-Guide-scramble is also provided as a negative control

Applications

  • Knock-in GFP reporter for promoter study
  • Knock-out genes at chromosomal level
Knockout Kit Finder
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CRISPR Manual CRISPR Manual PDF       Knockout Kit Validation Knockout Kit Validation


Scheme of Genome-editing knockout kit using CRISPR

Diagram-Gene Editing RecombII

Overview

The latest tool in genome editing – CRISPR/Cas9 – allows for specific genome disruption and replacement in a flexible and simple system resulting in high specificity and low cell toxicity. The CRISPR/Cas9 genome editing system requires the co- expression of a Cas9 protein with a guide RNA vector expressed from the human U6 polymerase III promoter. With the protospacer-adjacent motif (PAM - the sequence NGG) present at the 3′ end, Cas9 will unwind the DNA duplex and cleave both strands upon recognition of a target sequence by the guide RNA.

Learn CRISPR from our Youtube Channel OriGene YouTube Channel

Frequently Asked Questions

HA tagging Validation Data PDF

References

 

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