What is CRISPR?
CRISPR/Cas9, is an RNA-guided targeted genome editing tool – allows researchers to do gene knockout, knockin SNPs, insertions and deletions in cell lines and animals. The CRISPR/Cas9 genome editing system requires two components, Cas9, the endonuclease, and a guide RNA (sgRNA); sgRNA guides Cas9 to the location in the genome sequence specifically. With the protospacer-adjacent motif (PAM - the sequence NGG) present at the 3′ end, Cas9 will unwind the DNA duplex and cleave both strands upon recognition of a target sequence by the guide RNA.
Learn more from CRISPR videos.
CRISPR Products and Services
OriGene provides reagents to support a broad range of CRISPR/Cas9 genome editing applications. Click on any genome editing related product below and learn more about how we can help you.
Gene Knockout Kits
gRNA and donor cloning
AAVS Transgene Insertion
Knockout Cell Lysate
- Multiplex Genome Engineering Using CRISPR/Cas Systems. Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F.
Science 2013 Feb 15;339(6121):819-23
- RNA-Guided Human Genome Engineering via Cas9. Mali P, Yang L, Esvelt KM, Aach J, Guell M, DiCarlo JE, Norville JE, Church GM
Science 2013 Feb 15;339(6121):823-6