OriGene Technologies, Inc.
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home CRISPR/Cas9

CRISPR/Cas9 Genome Editing

divider

Click to view the details of CRISPR/CAS9
Genome-editing
Knockout Kit
CRISPR/Cas
Vectors
gRNA Cloning and
Donor Construction
CRISPR/Cas
Starter Kit

Features

Knockout Kit Finder
Search by gene symbol or keyword.
  • Complete kit for gene knockout via CRISPR (targeted sites around the 5’ end of the ORF)
  • 2 guide RNA vectors in pCas-Guide to ensure an efficient cleavage
  • Donor vector with predesigned homologous arms
  • Knockin GFP-Puro for selection
  • pCas-Guide-scramble is also provided as a negative control

Applications

  • Knock-in GFP reporter for promoter study
  • Knock-out genes at chromosomal level

Scheme of Genome-editing knockout kit using CRISPR

Diagram-Gene Editing RecombII

Overview

The latest tool in genome editing – CRISPR/Cas9 – allows for specific genome disruption and replacement in a flexible and simple system resulting in high specificity and low cell toxicity. The CRISPR/Cas9 genome editing system requires the co- expression of a Cas9 protein with a guide RNA vector expressed from the human U6 polymerase III promoter. With the protospacer-adjacent motif (PAM - the sequence NGG) present at the 3′ end, Cas9 will unwind the DNA duplex and cleave both strands upon recognition of a target sequence by the guide RNA.

Learn from a 4-min Youtube Video

Frequently Asked Questions

Validation data for pCas-Guide system PDF

References

 

bar
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title