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Home Primary Antibodies TrueMAB

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TrueMAB™ Monoclonal Antibodies

Made against Authentic Protein Antigens

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TrueMAB™ Antibody Features

Full-length Human Proteins as Antigens

TrueMAB™ antibodies are superior monoclonal antibodies made against authentic protein antigens (mostly full-length proteins expressed in human cell lines) and thus are able to recognize the native epitope(s) of the protein target. This feature makes TrueMAB a great tool for immunoassays that are sensitive to proteins’ conformations, such as IF/ICC, IP, flow, ELISA, IHC, high content screening (HCS), etc.

antibody
Extensive Validation
  • Western blot on cell lysates (9 cell lines with a positive control)
  • Immunohistochemistry on 24 human normal and tumor FFPE tissues
  • Immunofluorescent staining
  • Flow cytometry
  • Immuno-precipitation
  • ELISA
Free Positive Controls Included

Every TrueMAB™ antibody comes with a free positive control if applicable, which is the HEK293 over-expression lysate of the target protein. This free lysate can serve as the positive control in WB.

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OriGene has the capability to generate ~150 new TrueMAB™ antibodies each month. You can view our current offerings or nominate targets for new TrueMAB generation:

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TrueMAB™ monoclonal antibodies were generated using recombinant human proteins as antigens (mostly full-length proteins expressed in human cell lines) that were affinity purified under native condition to preserve the protein conformations. The commonly available commercial antibodies were generated using short peptides. The peptide antigens are inadequate to mimic the protein conformations due to the lack of three-dimensional structures. In particular, peptide-derived antibodies cannot recognize conformational epitopes which are primarily presented on the surfaces of native proteins.

In comparison to peptide-derived antibodies, TrueMAB™ monoclonal antibodies provide high sensitivity and specificity for the recognition of native epitopes appeared on the protein conformational structures. They are great tools for immunoassays that are sensitive to proteins’ conformations, such as immunofluorescence, immunoprecipitation, flow cytometry, ELISA, immunohistochemistry, high content screening (HCS), antibody arrays and more. For further questions, please send emails to antibody@origene.com or contact technical supports.

Native epitopes: The epitopes presented on the surfaces of protein’s native conformations. A. Conformational epitope (discontinuous epitope) ; B. Linear epitope. Conformational epitopes are abundant on the surfaces of native proteins. The peptide antigens are inadequate to mimic the protein conformations due to the lack of three-dimensional structures.

Epitope recognitions & applications

  • Linear epitopes of denatured proteins: Western blot
  • Native epitopes of proteins’ conformations (linear and conformational epitopes): Immunofluorescence, flow cytometry, immuno-precipitation, ChIP, ELISA, high content screening (HCS), antibody arrays & Luminex multiplexing
  • Conformational and linear epitopes of native/partially-unfolded proteins (fixed & cross-linked): Immunohistochemistry:
Applications Proteins Conformations in the Samples Epitope Recognition
Western blot Denatured Proteins Unfolded Linear Epitopes
Immunofluorescent Staining Native Folded Proteins Native Epitopes
Flow Cytometry Native Folded Proteins Native Epitopes
Immuno-precipitation Native Folded Proteins Native Epitopes
ELISA Native Folded Proteins Native Epitopes
High Content Screening Native Folded Proteins Native Epitopes
Antibody Array Native Folded Proteins Native Epitopes
Luminex Multiplexing Native Folded Proteins Native Epitopes
Immunohistochmeistry Native/Partially Unfolded Proteins (fixed & cross linked) Conformational & linear Epitopes

Cell lines and FFPE tissues for TrueMAB™ validation:

Cell lines: HeLa (human), HepG2 (human), COS7 (monkey), PC12 (rat), A549 (human), HT29 (Human), MDCK (canine), Jurkat (human), HEK293T (human), MCF-7 (human), SVT2 (mouse)

FFPE human normal tissues: paraffin-embedded human breast tissue, human colon tissue, human kidney tissue, human liver tissue, human lung tissue, human ovary tissue, human pancreas tissue, human thyroid tissue, human endometrium tissue, human prostate tissue, human bladder tissue, human lymph node tissue

FFPE human tumor tissues: paraffin-embedded adenocarcinoma of human breast tissue, adenocarcinoma of human colon tissue, carcinoma of human kidney tissue, carcinoma of human liver tissue, carcinoma of human lung tissue, adenocarcinoma of human ovary tissue, carcinoma of human pancreas tissue, carcinoma of human thyroid tissue, adenocarcinoma of human endometrium tissue, carcinoma of human prostate tissue, carcinoma of human bladder tissue, human lymphoma tissue

Immunofluorescent staining of transiently transfected COS7 cells

 

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