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Home Antibody All anti-BCAT1 antibodies

Anti-BCAT1 TRUEMAB Antibody Clone 3F5

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA504360
  • BCAT1 mouse monoclonal antibody,clone 3F5
  • FREE positive control: HEK293T cell transient overexpression lysate (LC401683) , 20ug
100ul 379 In Stock
CF504360
  • Carrier-free (BSA/glycerol-free) BCAT1 mouse monoclonal antibody,clone 3F5
100ug $450 3-4 weeks
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WB(2)
IHC(6)
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Also for BCAT1 (NM_005504)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human BCAT1(NP_005495) produced in HEK293T cell.
Clone NameClone 3F5 IsotypeIgG2a
Species ReactivityHuman , Dog , Mouse Concentration0.78 mg/ml
Guaranteed Application *WB, IHC Suggested DilutionsWB 1:500~2000, IHC 1:150,
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens branched chain amino-acid transaminase 1, cytosolic (BCAT1), transcript variant 1
Alternative NameBCATC; BCT1; ECA39; MECA39; PNAS121; PP18
Database LinkNP_005495
FunctionThis gene encodes the cytosolic form of the enzyme branched-chain amino acid transaminase. This enzyme catalyzes the reversible transamination of branched-chain alpha-keto acids to branched-chain L-amino acids essential for cell growth. Two different clinical disorders have been attributed to a defect of branched-chain amino acid transamination: hypervalinemia and hyperleucine-isoleucinemia. As there is also a gene encoding a mitochondrial form of this enzyme, mutations in either gene may contribute to these disorders. Alternatively spliced transcript variants have been described. [provided by RefSeq].
Related Pathway

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCAT1 (RC219229, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCAT1.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BCAT1 monoclonal antibody.
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-BCAT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA504360)
IHC Image
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-BCAT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA504360)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-BCAT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA504360)
IHC Image
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-BCAT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA504360)
IHC Image
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-BCAT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA504360)
IHC Image
Immunohistochemical staining of paraffin-embedded Human tonsil using anti-BCAT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA504360)

 

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