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Anti-DNAJB1 TRUEMAB Antibody Clone OTI1E9
TrueMAB Antibodies - Made against Authentic Protein Antigens
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Also for DNAJB1 (NM_006145)
|Full length human recombinant protein of human DNAJB1 (NP_006136) produced in HEK293T cell.|
|WB, IHC, IF, FC
||WB 1:1000~2000, IHC 1:150, IF 1:100, FLOW 1:100
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DNAJB1 (RC201762, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DNAJB1.
Western blot analysis of extracts (10ug) from 1 cell line by using anti-DNAJB1 monoclonal antibody. (1:200)
Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-DNAJB1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-DNAJB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502194)
Anti-DNAJB1 mouse monoclonal antibody (TA502194) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DNAJB1(RC201762).
HEK293T cells transfected with either RC201762 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-DNAJB1 antibody(TA502194), and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using anti-DNAJB1 antibody(TA502194),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).