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Home Antibody All anti-HSPB8 antibodies

Anti-HSPB8 TRUEMAB Antibody clone 1E3

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA501210
  • HSPB8 (Hsp22) mouse monoclonal antibody, clone 1E3
  • FREE positive control: HEK293T cell transient overexpression lysate (LC415329) , 20ug
100ul $379 In Stock
CF501210
  • Carrier-free (BSA/glycerol-free) HSPB8 mouse monoclonal antibody, clone 1E3
100ug $450 3-4 weeks
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WB(2)
IF(1)
FC(2)
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Also for HSPB8 (NM_014365)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human HSPB8(NP_055180) produced in HEK293T cell.
Clone Nameclone 1E3 IsotypeIgG2a
Species ReactivityHuman, Rat Concentration0.88 mg/ml
Guaranteed Application *WB, IF, FC Suggested DilutionsWB 1:500~2000, IF 1:100, FLOW 1:100
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens heat shock 22kDa protein 8 (HSPB8)
Alternative NameCMT2L; DHMN2; E2IG1; H11; HMN2; HMN2A; HSP22
Database LinkNP_055180
FunctionThe protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The expression of this gene in induced by estrogen in estrogen receptor-positive breast cancer cells, and this protein also functions as a chaperone in association with Bag3, a stimulator of macroautophagy. Thus, this gene appears to be involved in regulation of cell proliferation, apoptosis, and carcinogenesis, and mutations in this gene have been associated with different neuromuscular diseases, including Charcot-Marie-Tooth disease. [provided by RefSeq].
Related Pathway

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPB8 (RC201040, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPB8.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HSPB8 monoclonal antibody.
IF Image
Anti-HSPB8 mouse monoclonal antibody (TA501210) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HSPB8(RC201040 ).
FC Image
HEK293T cells transfected with either RC201040 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-HSPB8 antibody(TA501210), and then analyzed by flow cytometry.
FC Image
Flow cytometric Analysis of Jurkat cells, using anti-HSPB8 antibody(TA501210),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

 

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