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Home Antibody All anti-PIM2 antibodies

Anti-PIM2 TRUEMAB Antibody clone 9A5

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA501166
  • anti-PIM2 mouse monoclonal antibody, clone 9A5
  • FREE positive control: HEK293T cell transient overexpression lysate (LC416355) , 20ug
100ul 325 In Stock
CF501166
  • Carrier-free (BSA/glycerol-free) PIM2 mouse monoclonal antibody, clone 9A5
100ug $450 3-4 weeks
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WB(1)
IP(1)
IHC(3)
IF(1)
FC(3)
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Also for PIM2 (NM_006875)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human PIM2 (NP_006866) produced in HEK293T cell.
Clone Nameclone 9A5 IsotypeIgG2a
Species ReactivityHuman Concentration0.87mg/ml
Guaranteed Application *WB, IHC, IF, IP, FC Suggested DilutionsWB 1:2000, IHC 1:50, IF 1:100, FLOW 1:100, IP 2ug/500ul
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens pim-2 oncogene (PIM2)
Alternative Name
Database LinkNP_006866
FunctionThis gene encodes a protooncogene that acts as a serine/threonine protein kinase. Studies determined the encoded protein functions to prevent apoptosis and to promote cell survival.
Related Pathway

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PIM2 (RC201933, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PIM2.
IHC Image
Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-PIM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA501166)
IHC Image
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-PIM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA501166)
IHC Image
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-PIM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA501166)
IF Image
Anti-PIM2 mouse monoclonal antibody (TA501166) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PIM2(RC201933).
IP Image
Immunoprecipitation(IP) of PIM2 by using TrueMab monoclonal anti-PIM2 antibodies (Negative control: IP without adding anti-PIM2 antibody.). For each experiment, 500ul of DDK tagged PIM2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-PIM2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
FC Image
HEK293T cells transfected with either RC201933 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PIM2 antibody(TA501166), and then analyzed by flow cytometry.
FC Image
Flow cytometric Analysis of Hela cells, using anti-PIM2 antibody(TA501166),(Red), compared to a nonspecific negative control antibody,(Blue).
FC Image
Flow cytometric Analysis of Jurkat cells, using anti-PIM2 antibody(TA501166),(Red), compared to a nonspecific negative control antibody,(Blue).

 

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