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Home Antibody All anti-DAPK2 antibodies

Anti-DAPK2 TRUEMAB Antibody clone 1C5

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA501099
  • DAPK2 (DAP Kinase 2 ) mouse monoclonal antibody, clone 1C5
  • FREE positive control: HEK293T cell transient overexpression lysate (LC402315) , 20ug
100ul 379
$284
In Stock
CF501099
  • Carrier-free (BSA/glycerol-free) DAPK2 mouse monoclonal antibody, clone 1C5
100ug $450 3-4 weeks
Add to Shopping Cart
WB(2)
IP(1)
IHC(8)
IF(1)
FC(2)
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Also for DAPK2 (NM_014326)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human DAPK2 (NP_055141) produced in HEK293T cell.
Clone Nameclone 1C5 IsotypeIgG3
Species ReactivityHuman , Dog , Rat , Monkey Concentration0.76 mg/ml
Guaranteed Application *WB, IHC, IF, IP, FC Suggested DilutionsWB 1:1000~2000, IHC 1:50, IF 1:100, FLOW 1:100, IP 2ug/500ul
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens death-associated protein kinase 2 (DAPK2)
Alternative NameDRP-1; DRP1
Database LinkNP_055141
FunctionThis gene encodes a protein that belongs to the serine/threonine protein kinase family. This protein contains a N-terminal protein kinase domain followed by a conserved calmodulin-binding domain with significant similarity to that of death-associated protein kinase 1 (DAPK1), a positive regulator of programmed cell death. Overexpression of this gene was shown to induce cell apoptosis. It uses multiple polyadenylation sites. [provided by RefSeq].
Related Pathway

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DAPK2 (RC216274, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DAPK2.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-DAPK2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
IHC Image
Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-DAPK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA501099)
IHC Image
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-DAPK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA501099)
IHC Image
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-DAPK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA501099)
IHC Image
Immunohistochemical staining of paraffin-embedded Human lung tissue using anti-DAPK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA501099)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-DAPK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA501099)
IHC Image
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-DAPK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA501099)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-DAPK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA501099)
IHC Image
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-DAPK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA501099)
IF Image
Anti-DAPK2 mouse monoclonal antibody (TA501099) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DAPK2(RC216274).
IP Image
Immunoprecipitation(IP) of DAPK2 by using TrueMab monoclonal anti-DAPK2 antibodies (Negative control: IP without adding anti-DAPK2 antibody.). For each experiment, 500ul of DDK tagged DAPK2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-DAPK2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
FC Image
HEK293T cells transfected with either RC216274 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-DAPK2 antibody(TA501099), and then analyzed by flow cytometry.
FC Image
Flow cytometric Analysis of Jurkat cells, using anti-DAPK2 antibody(TA501099),(Red), compared to a nonspecific negative control antibody,(Blue).

 

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