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Home Antibody All anti-MAP2K4 antibodies

Anti-MAP2K4 TRUEMAB Antibody Clone OTI8A8

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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SKU Description Amount Price Availability*  
TA500403 anti-MAP2K4 (MEK4) mouse monoclonal antibody, clone OTI8A8 (formerly 8A8) 100ul $379 In Stock
LC401058 MAP2K4 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF500403 Carrier-free (BSA/glycerol-free) MAP2K4 mouse monoclonal antibody, clone OTI8A8 (formerly 8A8) 100ug $450 3-4 weeks
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WB(3)
IP(1)
IHC(5)
IF(2)
FC(1)
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Also for MAP2K4 (NM_003010)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull-length protein expressed in 293T cell transfected with human MAP2K4 expression vector
Clone NameClone OTI8A8 IsotypeIgG2b
Species ReactivityHuman , Dog Concentration2.36 mg/ml
Guaranteed Application *WB, IHC, IF, IP, FC Suggested DilutionsWB 1:500~1000, IHC 1:150, IF 1:50~100, FLOW 1:100, IP 2ug/500ul
Predicted MW Explanation 44.3 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens mitogen-activated protein kinase kinase 4 (MAP2K4), transcript variant 1
Alternative NameJNKK; JNKK1; MAPKK4; MEK4; MKK4; PRKMK4; SAPKK-1; SAPKK1; SEK1; SERK1; SKK1
Database LinkNP_003001
Entrez Gene 6416 Human
Entrez Gene 489508 Dog
FunctionThis gene encodes a dual specificity protein kinase that belongs to the Ser/Thr protein kinase family. This kinase is a direct activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. It has been shown to activate MAPK8/JNK1, MAPK9/JNK2, and MAPK14/p38, but not MAPK1/ERK2 or MAPK3/ERK3. This kinase is phosphorylated, and thus activated by MAP3K1/MEKK. The knockout studies in mice suggested the roles of this kinase in mediating survival signal in T cell development, as well as in the organogenesis of liver
Related PathwayProtein KinaseDruggable Genome MAPK signaling pathwayErbB signaling pathwayToll-like receptor signaling pathwayFc epsilon RI signaling pathwayGnRH signaling pathwayEpithelial cell signaling in Helicobacter pylori infectionMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAP2K4 (RC206051, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP2K4.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-MAP2K4 monoclonal antibody.
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Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-MAP2K4 monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-MAP2K4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500403)
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Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-MAP2K4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500403)
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MAP2K4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500403)
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Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-MAP2K4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500403)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MAP2K4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500403)
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Anti-MAP2K4 mouse monoclonal antibody (TA500403) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAP2K4(RC206051).
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Immunofluorescent staining of HepG2 cells using anti-MAP2K4 mouse monoclonal antibody (TA500403).
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Immunoprecipitation(IP) of MAP2K4 by using TrueMab monoclonal anti-MAP2K4 antibodies (Negative control: IP without adding anti-MAP2K4 antibody.). For each experiment, 500ul of DDK tagged MAP2K4 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-MAP2K4 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
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HEK293T cells transfected with either RC206051 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-MAP2K4 antibody(TA500403), and then analyzed by flow cytometry.
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