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Anti-NFATC3 PHOSPHO Antibody
Also for NFATC3 (NM_004555)
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.|
||WB: 1:500~1:3000, IF: 1:100~1:500, ELISA: 1:1000
|Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
|NFAT4 (Phospho-Ser165) antibody detects endogenous levels of NFAT4 only when phosphorylated at serine 165.
|Homo sapiens nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3 (NFATC3), transcript variant 2|
Wnt Signaling Pathway
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Western blot - NFAT4 (phospho S165) antibody. All lanes : Anti-NFAT4 (phospho S165) antibody at 1/500 dilution. Lane 1 : HeLa cells treated with calcium (40nM, 30 mins). Lane 2 : HeLa cells treated with calcium (40nM, 30 mins) with immunizing phosphopeptide. Predicted band size : 116 kDa. Observed band size : 116 kDa.
Immunofluorescence analysis of HeLa cells, using NFAT4 (Phospho-Ser165) antibody. Left: without immunizing phosphopeptide; Right: with immunizing phosphopeptide.