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Anti-NFKB2 Antibody EPR4686
Also for NFKB2 (NM_001077493)
|A recombinant protein fragment corresponding to residues in human NF-kappa-B p100 was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:10000 - 1:50000; IP: 1:10 - 1:100
|Does not react with Rat. Is unsuitable for Flow Cyt,ICC or IHC-P.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt,ICC or IHC-P.
|Homo sapiens nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100) (NFKB2), transcript variant 3|
|LYT-10; LYT10; p52|
|NF-kappa-B p100 has been detected in numerous cell types that express cytokines, chemokines, growth factors, cell adhesion molecules, and some acute phase proteins in health and in various disease states. It is activated by a wide variety of stimuli, such as cytokines, oxidant-free radicals, inhaled particles, ultraviolet irradiation, and bacterial or viral products. Inappropriate activation of NF-kappa-B p100 has been linked to inflammatory events associated with autoimmune arthritis, asthma, septic shock, lung fibrosis, glomerulonephritis, atherosclerosis, and AIDS. In contrast, complete and persistent inhibition of NF-kappa-B p100 has been linked directly to apoptosis, inappropriate immune cell development, and delayed cell growth. NF-kappa-B p100 and p105 proteins are processed to produce the active p50 and p52 NF-kappa-B subunits, respectively. However, the NF-kappa-B p100 and p105 proteins serve regulatory functions and should not be considered exclusively as precursor forms. The most abundant activated form of NF-kappa-B is a heterodimer of the p50 or p52 subunit bound to the RELA subunit. Other NF-kappa-B complexes, consisting of hetero- and homodimers of p50, p52, RELA, REL, and RELB, have also been detected. NF-kappa-B complexes are inhibited by I-kappa-B proteins, NFKBIA or NFKBIB, which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by the kinases IKBKA or IKBKB marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. The activated NF-kappa-B complex translocates into the nucleus and binds DNA at kappa-B-binding motifs, such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (1).|
MAPK signaling pathway
Toll-like receptor signaling pathway
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Western blot - NFkB p100 / p52 antibody [EPR4686]; All lanes : Anti-NFkB p100 / p52 [EPR4686] antibody at 1/10000 dilution.Lane 1 : Jurkat cell lysate.Lane 2 : HeLa cell lysate.Lane 3 : ECV-304 cell lysate.Lane 4 : MCF7 cell lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 97 kDa.Observed band size : 110 kDa .